Restriction map for
pFN21AE1362
(Linear) (Six-base) MAPSORT of: KIEE1362.seq Check: 5810 from: 1 to: 936
KIEE1362.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 600 936
Size: 600 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 505 667 936
Size: 205 300 162 269
Fragments arranged by size:
300 269 205 162
AfeI AGC'GCT
Cuts at: 0 400 936
Size: 400 536
AlwNI CAG_nnn'CTG
Cuts at: 0 183 804 852 936
Size: 183 621 48 84
Fragments arranged by size:
621 183 84 48
ApaI G_GGCC'C
Cuts at: 0 504 936
Size: 504 432
AvaI C'yCGr_G
Cuts at: 0 495 503 865 936
Size: 495 8 362 71
Fragments arranged by size:
495 362 71 8
BanI G'GyrC_C
Cuts at: 0 333 361 549 936
Size: 333 28 188 387
Fragments arranged by size:
387 333 188 28
BanII G_rGCy'C
Cuts at: 0 132 166 504 881 936
Size: 132 34 338 377 55
Fragments arranged by size:
377 338 132 55 34
BbeI G_GCGC'C
Cuts at: 0 365 936
Size: 365 571
BglI GCCn_nnn'nGGC
Cuts at: 0 435 936
Size: 435 501
BlpI GC'TnA_GC
Cuts at: 0 458 640 936
Size: 458 182 296
Fragments arranged by size:
458 296 182
Bme1580I G_kGCm'C
Cuts at: 0 23 504 554 936
Size: 23 481 50 382
Fragments arranged by size:
481 382 50 23
BmgBI CAC'GTC
Cuts at: 0 171 936
Size: 171 765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 484 936
Size: 367 117 452
Fragments arranged by size:
452 367 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 685 936
Size: 685 251
BsaHI Gr'CG_yC
Cuts at: 0 362 936
Size: 362 574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 315 409 883 936
Size: 315 94 474 53
Fragments arranged by size:
474 315 94 53
BseYI C'CCAG_C
Cuts at: 0 7 104 323 422 903 936
Size: 7 97 219 99 481 33
Fragments arranged by size:
481 219 99 97 33 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 838 936
Size: 238 600 98
Fragments arranged by size:
600 238 98
BsiEI CG_ry'CG
Cuts at: 0 314 435 637 936
Size: 314 121 202 299
Fragments arranged by size:
314 299 202 121
BsiHKAI G_wGCw'C
Cuts at: 0 881 936
Size: 881 55
Bsp1286I G_dGCh'C
Cuts at: 0 23 132 166 504 554 881 936
Size: 23 109 34 338 50 327 55
Fragments arranged by size:
338 327 109 55 50 34 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 936
Size: 600 336
BsrBI CCG'CTC
Cuts at: 0 155 369 936
Size: 155 214 567
Fragments arranged by size:
567 214 155
BsrFI r'CCGG_y
Cuts at: 0 358 434 936
Size: 358 76 502
Fragments arranged by size:
502 358 76
BsrGI T'GTAC_A
Cuts at: 0 122 936
Size: 122 814
BssHII G'CGCG_C
Cuts at: 0 285 354 667 936
Size: 285 69 313 269
Fragments arranged by size:
313 285 269 69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 333 936
Size: 333 603
BstYI r'GATC_y
Cuts at: 0 188 936
Size: 188 748
BtsI GCAGTG_nn'
Cuts at: 0 125 706 936
Size: 125 581 230
Fragments arranged by size:
581 230 125
DraIII CAC_nnn'GTG
Cuts at: 0 333 936
Size: 333 603
DrdI GACnn_nn'nnGTC
Cuts at: 0 76 520 936
Size: 76 444 416
Fragments arranged by size:
444 416 76
EaeI y'GGCC_r
Cuts at: 0 249 311 634 936
Size: 249 62 323 302
Fragments arranged by size:
323 302 249 62
EagI C'GGCC_G
Cuts at: 0 311 634 936
Size: 311 323 302
Fragments arranged by size:
323 311 302
EarI CTCTTCn'nnn_
Cuts at: 0 20 936
Size: 20 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 936
Size: 403 533
EcoICRI GAG'CTC
Cuts at: 0 879 936
Size: 879 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 367 484 505 667 936
Size: 205 162 117 21 162 269
Fragments arranged by size:
269 205 162 162 117 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 856 892 936
Size: 856 36 44
Fragments arranged by size:
856 44 36
EcoO109I rG'GnC_Cy
Cuts at: 0 500 735 936
Size: 500 235 201
Fragments arranged by size:
500 235 201
HaeII r_GCGC'y
Cuts at: 0 283 365 402 705 730 745 813 852
Size: 283 82 37 303 25 15 68 39
Cuts at: 852 936
Size: 84
Fragments arranged by size:
303 283 84 82 68 39 37 25
15
KasI G'GCGC_C
Cuts at: 0 361 936
Size: 361 575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 542 936
Size: 65 477 394
Fragments arranged by size:
477 394 65
MspA1I CmG'CkG
Cuts at: 0 7 210 222 339 639 801 936
Size: 7 203 12 117 300 162 135
Fragments arranged by size:
300 203 162 135 117 12 7
NaeI GCC'GGC
Cuts at: 0 360 936
Size: 360 576
NarI GG'CG_CC
Cuts at: 0 362 936
Size: 362 574
NgoMIV G'CCGG_C
Cuts at: 0 358 936
Size: 358 578
NotI GC'GGCC_GC
Cuts at: 0 311 936
Size: 311 625
PfoI T'CCnGG_A
Cuts at: 0 440 936
Size: 440 496
PpuMI rG'GwC_Cy
Cuts at: 0 735 936
Size: 735 201
PspOMI G'GGCC_C
Cuts at: 0 500 936
Size: 500 436
PspXI vC'TCGA_Gb
Cuts at: 0 865 936
Size: 865 71
PstI C_TGCA'G
Cuts at: 0 137 179 299 713 848 936
Size: 137 42 120 414 135 88
Fragments arranged by size:
414 137 135 120 88 42
PvuII CAG'CTG
Cuts at: 0 7 210 222 801 936
Size: 7 203 12 579 135
Fragments arranged by size:
579 203 135 12 7
SacI G_AGCT'C
Cuts at: 0 881 936
Size: 881 55
SanDI GG'GwC_CC
Cuts at: 0 735 936
Size: 735 201
SfcI C'TryA_G
Cuts at: 0 133 175 295 623 709 844 936
Size: 133 42 120 328 86 135 92
Fragments arranged by size:
328 135 133 120 92 86 42
SfoI GGC'GCC
Cuts at: 0 363 936
Size: 363 573
SgrAI Cr'CCGG_yG
Cuts at: 0 358 936
Size: 358 578
SmaI CCC'GGG
Cuts at: 0 505 936
Size: 505 431
SmlI C'TyrA_G
Cuts at: 0 865 936
Size: 865 71
StuI AGG'CCT
Cuts at: 0 793 936
Size: 793 143
StyI C'CwwG_G
Cuts at: 0 143 936
Size: 143 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 928 936
Size: 21 907 8
Fragments arranged by size:
907 21 8
TatI w'GTAC_w
Cuts at: 0 122 936
Size: 122 814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 927 936
Size: 927 9
XhoI C'TCGA_G
Cuts at: 0 865 936
Size: 865 71
XmaI C'CCGG_G
Cuts at: 0 503 936
Size: 503 433
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BmgBI BpmI BsaI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstYI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII KasI MmeI MspA1I NaeI NarI NgoMIV NotI
PfoI PpuMI PspOMI PspXI PstI PvuII SacI SanDI
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI MslI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XmnI ZraI