(Linear) (Six-base) MAPSORT of: KIEE1361.seq  Check: 1461  from: 1  to: 297



KIEE1361.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

ApoI r'AATT_y

Cuts at:      0     139     291     297 
   Size:        139     152       6

  Fragments arranged by size: 

                 152     139       6
 
BanII G_rGCy'C

Cuts at:      0     227     297 
   Size:        227      70
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     258     297 
   Size:        258      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     274     297 
   Size:         86     188      23

  Fragments arranged by size: 

                 188      86      23
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     297 
   Size:        227      70
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     297 
   Size:        227      70
 
BtgI C'CryG_G

Cuts at:      0      17     297 
   Size:         17     280
 
BtsI GCAGTG_nn'

Cuts at:      0     177     297 
   Size:        177     120
 
EaeI y'GGCC_r

Cuts at:      0      14     297 
   Size:         14     283
 
EarI CTCTTCn'nnn_

Cuts at:      0     213     274     297 
   Size:        213      61      23

  Fragments arranged by size: 

                 213      61      23
 
EcoICRI GAG'CTC

Cuts at:      0     225     297 
   Size:        225      72
 
EcoRI G'AATT_C

Cuts at:      0     291     297 
   Size:        291       6
 
HindIII A'AGCT_T

Cuts at:      0     205     297 
   Size:        205      92
 
PstI C_TGCA'G

Cuts at:      0      51     297 
   Size:         51     246
 
SacI G_AGCT'C

Cuts at:      0     227     297 
   Size:        227      70
 
SfcI C'TryA_G

Cuts at:      0      47     297 
   Size:         47     250
 
StyI C'CwwG_G

Cuts at:      0      76     239     297 
   Size:         76     163      58

  Fragments arranged by size: 

                 163      76      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     240     297 
   Size:        240      57
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      15     297 
   Size:         15     282
 

 Enzymes that do cut:

     ApoI    BanII     BmrI     BsgI  BsiHKAI Bsp1286I     BtgI     BtsI
     EaeI     EarI  EcoICRI    EcoRI  HindIII     PstI     SacI     SfcI
     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI