(Linear) (Six-base) MAPSORT of: KIEE1356.seq  Check: 9934  from: 1  to: 273



KIEE1356.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     273 
   Size:        205      68
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      84     273 
   Size:         84     189
 
AvaI C'yCGr_G

Cuts at:      0      72     273 
   Size:         72     201
 
BanII G_rGCy'C

Cuts at:      0     180     273 
   Size:        180      93
 
BclI T'GATC_A

Cuts at:      0     153     273 
   Size:        153     120
 
BglII A'GATC_T

Cuts at:      0      65     273 
   Size:         65     208
 
BlpI GC'TnA_GC

Cuts at:      0     179     273 
   Size:        179      94
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     273 
   Size:        102     171
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     100     130     273 
   Size:        100      30     143

  Fragments arranged by size: 

                 143     100      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     273 
   Size:         91     182
 
Bsp1286I G_dGCh'C

Cuts at:      0     180     273 
   Size:        180      93
 
BstYI r'GATC_y

Cuts at:      0      65     273 
   Size:         65     208
 
BtgI C'CryG_G

Cuts at:      0     134     273 
   Size:        134     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     205     273 
   Size:        102     103      68

  Fragments arranged by size: 

                 103     102      68
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132     212     244     273 
   Size:        100      32      80      32      29

  Fragments arranged by size: 

                 100      80      32      32      29
 
MspA1I CmG'CkG

Cuts at:      0     183     273 
   Size:        183      90
 
NcoI C'CATG_G

Cuts at:      0     134     273 
   Size:        134     139
 
PstI C_TGCA'G

Cuts at:      0      49      63     273 
   Size:         49      14     210

  Fragments arranged by size: 

                 210      49      14
 
PvuII CAG'CTG

Cuts at:      0     183     273 
   Size:        183      90
 
SfcI C'TryA_G

Cuts at:      0      45      59     273 
   Size:         45      14     214

  Fragments arranged by size: 

                 214      45      14
 
StyI C'CwwG_G

Cuts at:      0     134     273 
   Size:        134     139
 

 Enzymes that do cut:

     AcuI     AlfI     AvaI    BanII     BclI    BglII     BlpI     BpmI
    BsaXI    BseRI Bsp1286I    BstYI     BtgI  Eco57MI    Hin4I   MspA1I
     NcoI     PstI    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI