Restriction map for
pFN21AE1354
(Linear) (Six-base) MAPSORT of: KIEE1354.seq Check: 7427 from: 1 to: 2733
KIEE1354.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 197 1955 2733
Size: 197 1758 778
Fragments arranged by size:
1758 778 197
AccI GT'mk_AC
Cuts at: 0 2453 2733
Size: 2453 280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 2089 2733
Size: 1327 762 644
Fragments arranged by size:
1327 762 644
AfeI AGC'GCT
Cuts at: 0 344 2733
Size: 344 2389
AflIII A'CryG_T
Cuts at: 0 1658 2733
Size: 1658 1075
AhdI GACnn_n'nnGTC
Cuts at: 0 1529 2502 2694 2733
Size: 1529 973 192 39
Fragments arranged by size:
1529 973 192 39
AlwNI CAG_nnn'CTG
Cuts at: 0 993 1884 2733
Size: 993 891 849
Fragments arranged by size:
993 891 849
ApaI G_GGCC'C
Cuts at: 0 1988 2360 2665 2733
Size: 1988 372 305 68
Fragments arranged by size:
1988 372 305 68
ApaLI G'TGCA_C
Cuts at: 0 316 1021 2733
Size: 316 705 1712
Fragments arranged by size:
1712 705 316
AvaI C'yCGr_G
Cuts at: 0 2359 2733
Size: 2359 374
BamHI G'GATC_C
Cuts at: 0 923 1991 2733
Size: 923 1068 742
Fragments arranged by size:
1068 923 742
BanI G'GyrC_C
Cuts at: 0 515 1481 1887 2649 2680 2733
Size: 515 966 406 762 31 53
Fragments arranged by size:
966 762 515 406 53 31
BanII G_rGCy'C
Cuts at: 0 245 506 1439 1988 2360 2665 2733
Size: 245 261 933 549 372 305 68
Fragments arranged by size:
933 549 372 305 261 245 68
BbeI G_GCGC'C
Cuts at: 0 2653 2733
Size: 2653 80
BbvCI CC'TCA_GC
Cuts at: 0 643 1096 2591 2733
Size: 643 453 1495 142
Fragments arranged by size:
1495 643 453 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 192 226 1372 1406 2733
Size: 192 34 1146 34 1327
Fragments arranged by size:
1327 1146 192 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1762 1969 2647 2733
Size: 1762 207 678 86
Fragments arranged by size:
1762 678 207 86
BglII A'GATC_T
Cuts at: 0 1490 2733
Size: 1490 1243
BlpI GC'TnA_GC
Cuts at: 0 2059 2733
Size: 2059 674
Bme1580I G_kGCm'C
Cuts at: 0 320 962 1025 1988 2360 2665 2678 2685
Size: 320 642 63 963 372 305 13 7
Cuts at: 2685 2733
Size: 48
Fragments arranged by size:
963 642 372 320 305 63 48 13
7
BmgBI CAC'GTC
Cuts at: 0 2400 2733
Size: 2400 333
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 2733
Size: 120 2613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 2733
Size: 259 2474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 937 967 1015 1078 1243 1567 1588
Size: 283 654 30 48 63 165 324 21
Cuts at: 1588 1636 1933 2068 2140 2733
Size: 48 297 135 72 593
Fragments arranged by size:
654 593 324 297 283 165 135 72
63 48 48 30 21
Bpu10I CC'TnA_GC
Cuts at: 0 290 643 1096 2591 2733
Size: 290 353 453 1495 142
Fragments arranged by size:
1495 453 353 290 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 2733
Size: 229 2504
BsaI GGTCTCn'nnnn_
Cuts at: 0 384 478 1143 2506 2733
Size: 384 94 665 1363 227
Fragments arranged by size:
1363 665 384 227 94
BsaHI Gr'CG_yC
Cuts at: 0 368 1508 1736 2273 2314 2650 2669 2733
Size: 368 1140 228 537 41 336 19 64
Fragments arranged by size:
1140 537 368 336 228 64 41 19
BsaWI w'CCGG_w
Cuts at: 0 836 2733
Size: 836 1897
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1444 1474 2733
Size: 1444 30 1259
Fragments arranged by size:
1444 1259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1207 1696 1930 2023 2377 2608 2733
Size: 1207 489 234 93 354 231 125
Fragments arranged by size:
1207 489 354 234 231 125 93
BseYI C'CCAG_C
Cuts at: 0 43 2204 2352 2733
Size: 43 2161 148 381
Fragments arranged by size:
2161 381 148 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1060 1570 1897 2733
Size: 574 486 510 327 836
Fragments arranged by size:
836 574 510 486 327
BsiEI CG_ry'CG
Cuts at: 0 2412 2733
Size: 2412 321
BsiHKAI G_wGCw'C
Cuts at: 0 320 878 1025 1439 1946 2192 2733
Size: 320 558 147 414 507 246 541
Fragments arranged by size:
558 541 507 414 320 246 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 230 1318 2733
Size: 230 1088 1415
Fragments arranged by size:
1415 1088 230
Bsp1286I G_dGCh'C
Cuts at: 0 245 320 506 878 962 1025 1439 1946
Size: 245 75 186 372 84 63 414 507
Cuts at: 1946 1988 2192 2360 2665 2678 2685 2733
Size: 42 204 168 305 13 7 48
Fragments arranged by size:
507 414 372 305 245 204 186 168
84 75 63 48 42 13 7
BspHI T'CATG_A
Cuts at: 0 2441 2733
Size: 2441 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 197 1955 2123 2733
Size: 131 66 1758 168 610
Fragments arranged by size:
1758 610 168 131 66
BsrBI CCG'CTC
Cuts at: 0 282 300 1227 2733
Size: 282 18 927 1506
Fragments arranged by size:
1506 927 282 18
BsrDI GCAATG_nn'
Cuts at: 0 63 1424 2225 2733
Size: 63 1361 801 508
Fragments arranged by size:
1361 801 508 63
BsrFI r'CCGG_y
Cuts at: 0 608 1013 1103 2411 2733
Size: 608 405 90 1308 322
Fragments arranged by size:
1308 608 405 322 90
BssHII G'CGCG_C
Cuts at: 0 1605 2733
Size: 1605 1128
BssSI C'ACGA_G
Cuts at: 0 1273 1366 2733
Size: 1273 93 1367
Fragments arranged by size:
1367 1273 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1887 2733
Size: 1887 846
BstBI TT'CG_AA
Cuts at: 0 683 2733
Size: 683 2050
BstEII G'GTnAC_C
Cuts at: 0 284 2733
Size: 284 2449
BstXI CCAn_nnnn'nTGG
Cuts at: 0 720 1980 2733
Size: 720 1260 753
Fragments arranged by size:
1260 753 720
BstYI r'GATC_y
Cuts at: 0 839 923 1211 1490 1991 2258 2733
Size: 839 84 288 279 501 267 475
Fragments arranged by size:
839 501 475 288 279 267 84
Bsu36I CC'TnA_GG
Cuts at: 0 451 2733
Size: 451 2282
BtgI C'CryG_G
Cuts at: 0 476 1640 2183 2705 2733
Size: 476 1164 543 522 28
Fragments arranged by size:
1164 543 522 476 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2529 2622 2733
Size: 2529 93 111
Fragments arranged by size:
2529 111 93
DraIII CAC_nnn'GTG
Cuts at: 0 1481 2733
Size: 1481 1252
DrdI GACnn_nn'nnGTC
Cuts at: 0 658 780 2733
Size: 658 122 1953
Fragments arranged by size:
1953 658 122
EaeI y'GGCC_r
Cuts at: 0 491 902 1328 1763 2733
Size: 491 411 426 435 970
Fragments arranged by size:
970 491 435 426 411
EarI CTCTTCn'nnn_
Cuts at: 0 389 476 2733
Size: 389 87 2257
Fragments arranged by size:
2257 389 87
EcoICRI GAG'CTC
Cuts at: 0 1437 2733
Size: 1437 1296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 937 967 1015 1078 1243 1327 1567
Size: 283 654 30 48 63 165 84 240
Cuts at: 1567 1588 1636 1933 2068 2089 2140 2733
Size: 21 48 297 135 21 51 593
Fragments arranged by size:
654 593 297 283 240 165 135 84
63 51 48 48 30 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 883 1912 2733
Size: 193 690 1029 821
Fragments arranged by size:
1029 821 690 193
EcoO109I rG'GnC_Cy
Cuts at: 0 120 1647 1692 1984 1985 2042 2148 2356
Size: 120 1527 45 292 1 57 106 208
Cuts at: 2356 2661 2662 2733
Size: 305 1 71
Fragments arranged by size:
1527 305 292 208 120 106 71 57
45 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 523 2733
Size: 523 2210
FspI TGC'GCA
Cuts at: 0 1394 2733
Size: 1394 1339
HaeII r_GCGC'y
Cuts at: 0 65 346 1640 2353 2653 2733
Size: 65 281 1294 713 300 80
Fragments arranged by size:
1294 713 300 281 80 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 1515 1547 2488 2520 2680 2712
Size: 256 32 1227 32 941 32 160 32
Cuts at: 2712 2733
Size: 21
Fragments arranged by size:
1227 941 256 160 32 32 32 32
21
HincII GTy'rAC
Cuts at: 0 861 1473 2454 2733
Size: 861 612 981 279
Fragments arranged by size:
981 861 612 279
KasI G'GCGC_C
Cuts at: 0 2649 2733
Size: 2649 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1961 2733
Size: 1961 772
MscI TGG'CCA
Cuts at: 0 904 1765 2733
Size: 904 861 968
Fragments arranged by size:
968 904 861
MslI CAynn'nnrTG
Cuts at: 0 475 2032 2197 2733
Size: 475 1557 165 536
Fragments arranged by size:
1557 536 475 165
MspA1I CmG'CkG
Cuts at: 0 572 1056 1566 1584 1884 2058 2214 2590
Size: 572 484 510 18 300 174 156 376
Cuts at: 2590 2733
Size: 143
Fragments arranged by size:
572 510 484 376 300 174 156 143
18
NarI GG'CG_CC
Cuts at: 0 2650 2733
Size: 2650 83
NcoI C'CATG_G
Cuts at: 0 1640 2183 2733
Size: 1640 543 550
Fragments arranged by size:
1640 550 543
NspI r_CATG'y
Cuts at: 0 1662 2733
Size: 1662 1071
PasI CC'CwG_GG
Cuts at: 0 426 2733
Size: 426 2307
PciI A'CATG_T
Cuts at: 0 1658 2733
Size: 1658 1075
PflMI CCAn_nnn'nTGG
Cuts at: 0 941 2733
Size: 941 1792
PfoI T'CCnGG_A
Cuts at: 0 260 464 914 974 1652 1910 2141 2733
Size: 260 204 450 60 678 258 231 592
Fragments arranged by size:
678 592 450 260 258 231 204 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2413 2445 2733
Size: 2413 32 288
Fragments arranged by size:
2413 288 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 1647 2042 2733
Size: 120 1527 395 691
Fragments arranged by size:
1527 691 395 120
PspOMI G'GGCC_C
Cuts at: 0 1984 2356 2661 2733
Size: 1984 372 305 72
Fragments arranged by size:
1984 372 305 72
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 13 45 2733
Size: 13 32 2688
Fragments arranged by size:
2688 32 13
PstI C_TGCA'G
Cuts at: 0 1082 2733
Size: 1082 1651
PvuII CAG'CTG
Cuts at: 0 572 1056 1566 1584 1884 2058 2214 2590
Size: 572 484 510 18 300 174 156 376
Cuts at: 2590 2733
Size: 143
Fragments arranged by size:
572 510 484 376 300 174 156 143
18
SacI G_AGCT'C
Cuts at: 0 1439 2733
Size: 1439 1294
SalI G'TCGA_C
Cuts at: 0 2452 2733
Size: 2452 281
SanDI GG'GwC_CC
Cuts at: 0 2042 2733
Size: 2042 691
ScaI AGT'ACT
Cuts at: 0 2632 2733
Size: 2632 101
SexAI A'CCwGG_T
Cuts at: 0 2537 2733
Size: 2537 196
SfcI C'TryA_G
Cuts at: 0 1078 2733
Size: 1078 1655
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1762 2733
Size: 1762 971
SfoI GGC'GCC
Cuts at: 0 2651 2733
Size: 2651 82
SmlI C'TyrA_G
Cuts at: 0 244 2733
Size: 244 2489
StuI AGG'CCT
Cuts at: 0 1264 1279 1372 2374 2733
Size: 1264 15 93 1002 359
Fragments arranged by size:
1264 1002 359 93 15
StyI C'CwwG_G
Cuts at: 0 494 1034 1640 2183 2276 2733
Size: 494 540 606 543 93 457
Fragments arranged by size:
606 543 540 494 457 93
TatI w'GTAC_w
Cuts at: 0 2630 2733
Size: 2630 103
Tth111I GACn'n_nGTC
Cuts at: 0 658 2539 2733
Size: 658 1881 194
Fragments arranged by size:
1881 658 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 912 942 2181 2244 2733
Size: 912 30 1239 63 489
Fragments arranged by size:
1239 912 489 63 30
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI
PsrI PstI PvuII SacI SalI SanDI ScaI SexAI
SfcI SfiI SfoI SmlI StuI StyI TatI Tth111I
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI
BclI BfrBI BmtI BsaAI BsaBI BsiWI BsmI BspEI
BsrGI BstZ17I BtsI ClaI CspCI DraI EagI EciI
EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PshAI PsiI PspXI PvuI RsrII
SacII SapI SbfI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI XbaI XhoI
XmaI XmnI ZraI