Restriction map for
pFN21AE1346
(Linear) (Six-base) MAPSORT of: KIEE1346.seq Check: 6653 from: 1 to: 765
KIEE1346.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 765
Size: 97 668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 207 765
Size: 207 558
ApaLI G'TGCA_C
Cuts at: 0 423 765
Size: 423 342
AvaI C'yCGr_G
Cuts at: 0 412 765
Size: 412 353
BanI G'GyrC_C
Cuts at: 0 223 765
Size: 223 542
BanII G_rGCy'C
Cuts at: 0 396 765
Size: 396 369
BbeI G_GCGC'C
Cuts at: 0 227 765
Size: 227 538
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 672 765
Size: 173 499 93
Fragments arranged by size:
499 173 93
BbvCI CC'TCA_GC
Cuts at: 0 47 765
Size: 47 718
BlpI GC'TnA_GC
Cuts at: 0 340 765
Size: 340 425
Bme1580I G_kGCm'C
Cuts at: 0 427 765
Size: 427 338
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 370 765
Size: 50 320 395
Fragments arranged by size:
395 320 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 765
Size: 656 109
Bpu10I CC'TnA_GC
Cuts at: 0 47 765
Size: 47 718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 765
Size: 678 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 402 696 748 765
Size: 402 294 52 17
Fragments arranged by size:
402 294 52 17
BsaHI Gr'CG_yC
Cuts at: 0 224 765
Size: 224 541
BsaWI w'CCGG_w
Cuts at: 0 601 765
Size: 601 164
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 471 765
Size: 471 294
BseYI C'CCAG_C
Cuts at: 0 194 254 437 765
Size: 194 60 183 328
Fragments arranged by size:
328 194 183 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 517 765
Size: 505 12 248
Fragments arranged by size:
505 248 12
BsiHKAI G_wGCw'C
Cuts at: 0 427 765
Size: 427 338
BsmBI CGTCTCn'nnnn_
Cuts at: 0 234 648 765
Size: 234 414 117
Fragments arranged by size:
414 234 117
Bsp1286I G_dGCh'C
Cuts at: 0 396 427 765
Size: 396 31 338
Fragments arranged by size:
396 338 31
BspEI T'CCGG_A
Cuts at: 0 601 765
Size: 601 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 765
Size: 121 644
BsrDI GCAATG_nn'
Cuts at: 0 189 765
Size: 189 576
BsrFI r'CCGG_y
Cuts at: 0 433 566 765
Size: 433 133 199
Fragments arranged by size:
433 199 133
BssHII G'CGCG_C
Cuts at: 0 271 765
Size: 271 494
BstYI r'GATC_y
Cuts at: 0 116 765
Size: 116 649
BtgI C'CryG_G
Cuts at: 0 633 765
Size: 633 132
BtsI GCAGTG_nn'
Cuts at: 0 266 517 765
Size: 266 251 248
Fragments arranged by size:
266 251 248
EaeI y'GGCC_r
Cuts at: 0 431 765
Size: 431 334
EarI CTCTTCn'nnn_
Cuts at: 0 342 765
Size: 342 423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 765
Size: 274 491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 656 765
Size: 97 559 109
Fragments arranged by size:
559 109 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 711 765
Size: 711 54
EcoO109I rG'GnC_Cy
Cuts at: 0 83 607 765
Size: 83 524 158
Fragments arranged by size:
524 158 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 765
Size: 603 162
HaeII r_GCGC'y
Cuts at: 0 227 765
Size: 227 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 171 203 765
Size: 108 32 31 32 562
Fragments arranged by size:
562 108 32 32 31
HindIII A'AGCT_T
Cuts at: 0 729 765
Size: 729 36
KasI G'GCGC_C
Cuts at: 0 223 765
Size: 223 542
MspA1I CmG'CkG
Cuts at: 0 306 339 489 765
Size: 306 33 150 276
Fragments arranged by size:
306 276 150 33
NaeI GCC'GGC
Cuts at: 0 435 765
Size: 435 330
NarI GG'CG_CC
Cuts at: 0 224 765
Size: 224 541
NgoMIV G'CCGG_C
Cuts at: 0 433 765
Size: 433 332
PasI CC'CwG_GG
Cuts at: 0 219 765
Size: 219 546
PvuII CAG'CTG
Cuts at: 0 339 489 765
Size: 339 150 276
Fragments arranged by size:
339 276 150
SfoI GGC'GCC
Cuts at: 0 225 765
Size: 225 540
SmaI CCC'GGG
Cuts at: 0 414 765
Size: 414 351
SmlI C'TyrA_G
Cuts at: 0 693 765
Size: 693 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 765
Size: 268 497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 765
Size: 496 32 237
Fragments arranged by size:
496 237 32
XmaI C'CCGG_G
Cuts at: 0 412 765
Size: 412 353
Enzymes that do cut:
AcuI AlfI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrFI BssHII BstYI BtgI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII KasI MspA1I NaeI NarI NgoMIV PasI
PvuII SfoI SmaI SmlI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI BsaAI BsaBI
BsaXI BsiEI BsiWI BsmI BspHI BsrBI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MmeI MscI MslI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI