Restriction map for
pFN21AE1339
(Linear) (Six-base) MAPSORT of: KIEE1339.seq Check: 4636 from: 1 to: 1560
KIEE1339.orf
With 182 enzymes: SgfI *
December 17, 2009 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 528 1105 1560
Size: 528 577 455
Fragments arranged by size:
577 528 455
AccI GT'mk_AC
Cuts at: 0 401 1560
Size: 401 1159
Acc65I G'GTAC_C
Cuts at: 0 1321 1560
Size: 1321 239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 1560
Size: 1325 235
AhdI GACnn_n'nnGTC
Cuts at: 0 273 1560
Size: 273 1287
AleI CACnn'nnGTG
Cuts at: 0 953 1560
Size: 953 607
AlwNI CAG_nnn'CTG
Cuts at: 0 1111 1560
Size: 1111 449
ApaI G_GGCC'C
Cuts at: 0 931 1410 1560
Size: 931 479 150
Fragments arranged by size:
931 479 150
ApoI r'AATT_y
Cuts at: 0 14 1356 1560
Size: 14 1342 204
Fragments arranged by size:
1342 204 14
AvaI C'yCGr_G
Cuts at: 0 573 1015 1560
Size: 573 442 545
Fragments arranged by size:
573 545 442
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1305 1338 1560
Size: 1305 33 222
Fragments arranged by size:
1305 222 33
BanI G'GyrC_C
Cuts at: 0 451 496 658 955 1321 1560
Size: 451 45 162 297 366 239
Fragments arranged by size:
451 366 297 239 162 45
BanII G_rGCy'C
Cuts at: 0 35 825 931 1015 1141 1167 1410 1560
Size: 35 790 106 84 126 26 243 150
Fragments arranged by size:
790 243 150 126 106 84 35 26
BbeI G_GCGC'C
Cuts at: 0 455 1560
Size: 455 1105
BbsI GAAGACnn'nnnn_
Cuts at: 0 454 1365 1560
Size: 454 911 195
Fragments arranged by size:
911 454 195
BbvCI CC'TCA_GC
Cuts at: 0 263 1560
Size: 263 1297
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1560
Size: 541 1019
BglII A'GATC_T
Cuts at: 0 1439 1560
Size: 1439 121
Bme1580I G_kGCm'C
Cuts at: 0 501 931 1410 1471 1560
Size: 501 430 479 61 89
Fragments arranged by size:
501 479 430 89 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 1442 1560
Size: 1442 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 326 620 839 902 1560
Size: 250 76 294 219 63 658
Fragments arranged by size:
658 294 250 219 76 63
Bpu10I CC'TnA_GC
Cuts at: 0 263 320 1560
Size: 263 57 1240
Fragments arranged by size:
1240 263 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 57 1560
Size: 4 53 1503
Fragments arranged by size:
1503 53 4
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 709 743 763 1184 1560
Size: 37 672 34 20 421 376
Fragments arranged by size:
672 421 376 37 34 20
BsaHI Gr'CG_yC
Cuts at: 0 452 1560
Size: 452 1108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 822 852 1560
Size: 822 30 708
Fragments arranged by size:
822 708 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 1546 1560
Size: 126 1420 14
Fragments arranged by size:
1420 126 14
BseYI C'CCAG_C
Cuts at: 0 178 1386 1505 1560
Size: 178 1208 119 55
Fragments arranged by size:
1208 178 119 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1476 1560
Size: 959 517 84
Fragments arranged by size:
959 517 84
BsiHKAI G_wGCw'C
Cuts at: 0 35 386 636 1560
Size: 35 351 250 924
Fragments arranged by size:
924 351 250 35
Bsp1286I G_dGCh'C
Cuts at: 0 35 386 501 636 825 931 1015 1141
Size: 35 351 115 135 189 106 84 126
Cuts at: 1141 1167 1410 1471 1560
Size: 26 243 61 89
Fragments arranged by size:
351 243 189 135 126 115 106 89
84 61 35 26
BspHI T'CATG_A
Cuts at: 0 1515 1560
Size: 1515 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 528 1105 1141 1560
Size: 528 577 36 419
Fragments arranged by size:
577 528 419 36
BsrDI GCAATG_nn'
Cuts at: 0 80 1560
Size: 80 1480
BsrGI T'GTAC_A
Cuts at: 0 1412 1560
Size: 1412 148
BssSI C'ACGA_G
Cuts at: 0 1528 1560
Size: 1528 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 390 1560
Size: 345 45 1170
Fragments arranged by size:
1170 345 45
BstBI TT'CG_AA
Cuts at: 0 86 1560
Size: 86 1474
BstYI r'GATC_y
Cuts at: 0 1207 1439 1560
Size: 1207 232 121
Fragments arranged by size:
1207 232 121
BtgI C'CryG_G
Cuts at: 0 482 1560
Size: 482 1078
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 710 1355 1560
Size: 710 645 205
Fragments arranged by size:
710 645 205
BtsI GCAGTG_nn'
Cuts at: 0 947 981 1560
Size: 947 34 579
Fragments arranged by size:
947 579 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 848 883 1560
Size: 848 35 677
Fragments arranged by size:
848 677 35
EaeI y'GGCC_r
Cuts at: 0 1179 1560
Size: 1179 381
EcoICRI GAG'CTC
Cuts at: 0 33 1560
Size: 33 1527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 326 620 839 902 1325 1560
Size: 250 76 294 219 63 423 235
Fragments arranged by size:
423 294 250 235 219 76 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1560
Size: 319 1241
EcoO109I rG'GnC_Cy
Cuts at: 0 225 568 577 622 667 811 928 1108
Size: 225 343 9 45 45 144 117 180
Cuts at: 1108 1153 1406 1407 1560
Size: 45 253 1 153
Fragments arranged by size:
343 253 225 180 153 144 117 45
45 45 9 1
HaeII r_GCGC'y
Cuts at: 0 179 455 1560
Size: 179 276 1105
Fragments arranged by size:
1105 276 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 1560
Size: 259 32 1269
Fragments arranged by size:
1269 259 32
KasI G'GCGC_C
Cuts at: 0 451 1560
Size: 451 1109
KpnI G_GTAC'C
Cuts at: 0 1325 1560
Size: 1325 235
MscI TGG'CCA
Cuts at: 0 1181 1560
Size: 1181 379
MslI CAynn'nnrTG
Cuts at: 0 487 953 1560
Size: 487 466 607
Fragments arranged by size:
607 487 466
MspA1I CmG'CkG
Cuts at: 0 172 396 1560
Size: 172 224 1164
Fragments arranged by size:
1164 224 172
NarI GG'CG_CC
Cuts at: 0 452 1560
Size: 452 1108
NcoI C'CATG_G
Cuts at: 0 482 1560
Size: 482 1078
NspI r_CATG'y
Cuts at: 0 492 1386 1560
Size: 492 894 174
Fragments arranged by size:
894 492 174
PasI CC'CwG_GG
Cuts at: 0 421 627 825 988 1141 1560
Size: 421 206 198 163 153 419
Fragments arranged by size:
421 419 206 198 163 153
PfoI T'CCnGG_A
Cuts at: 0 227 816 879 1023 1560
Size: 227 589 63 144 537
Fragments arranged by size:
589 537 227 144 63
PpuMI rG'GwC_Cy
Cuts at: 0 225 577 622 667 1153 1560
Size: 225 352 45 45 486 407
Fragments arranged by size:
486 407 352 225 45 45
PshAI GACnn'nnGTC
Cuts at: 0 755 1560
Size: 755 805
PspOMI G'GGCC_C
Cuts at: 0 927 1406 1560
Size: 927 479 154
Fragments arranged by size:
927 479 154
PstI C_TGCA'G
Cuts at: 0 212 293 350 395 917 1116 1525 1560
Size: 212 81 57 45 522 199 409 35
Fragments arranged by size:
522 409 212 199 81 57 45 35
SacI G_AGCT'C
Cuts at: 0 35 1560
Size: 35 1525
SanDI GG'GwC_CC
Cuts at: 0 577 1560
Size: 577 983
SbfI CC_TGCA'GG
Cuts at: 0 350 1116 1560
Size: 350 766 444
Fragments arranged by size:
766 444 350
SexAI A'CCwGG_T
Cuts at: 0 861 1560
Size: 861 699
SfcI C'TryA_G
Cuts at: 0 208 289 346 391 913 1112 1314 1521
Size: 208 81 57 45 522 199 202 207
Cuts at: 1521 1560
Size: 39
Fragments arranged by size:
522 208 207 202 199 81 57 45
39
SfoI GGC'GCC
Cuts at: 0 453 1560
Size: 453 1107
SmaI CCC'GGG
Cuts at: 0 1017 1560
Size: 1017 543
SmlI C'TyrA_G
Cuts at: 0 19 36 1560
Size: 19 17 1524
Fragments arranged by size:
1524 19 17
SphI G_CATG'C
Cuts at: 0 492 1386 1560
Size: 492 894 174
Fragments arranged by size:
894 492 174
StuI AGG'CCT
Cuts at: 0 1037 1560
Size: 1037 523
StyI C'CwwG_G
Cuts at: 0 482 536 582 618 672 959 1157 1560
Size: 482 54 46 36 54 287 198 403
Fragments arranged by size:
482 403 287 198 54 54 46 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 965 1560
Size: 965 595
TatI w'GTAC_w
Cuts at: 0 1412 1560
Size: 1412 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 822 854 1560
Size: 822 32 706
Fragments arranged by size:
822 706 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 1560
Size: 543 1017
XmaI C'CCGG_G
Cuts at: 0 1015 1560
Size: 1015 545
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlwNI ApaI
ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI EaeI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI KpnI MscI MslI MspA1I NarI
NcoI NspI PasI PfoI PpuMI PshAI PspOMI PstI
SacI SanDI SbfI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BamHI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII
DrdI EagI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SapI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI