(Linear) (Six-base) MAPSORT of: KIEE1337.seq  Check: 5295  from: 1  to: 474



KIEE1337.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AflII C'TTAA_G

Cuts at:      0      70     474 
   Size:         70     404
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     298     330     474 
   Size:        298      32     144

  Fragments arranged by size: 

                 298     144      32
 
AvrII C'CTAG_G

Cuts at:      0     225     474 
   Size:        225     249
 
BbvCI CC'TCA_GC

Cuts at:      0     218     474 
   Size:        218     256
 
BmgBI CAC'GTC

Cuts at:      0     343     474 
   Size:        343     131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     319     474 
   Size:        185     134     155

  Fragments arranged by size: 

                 185     155     134
 
Bpu10I CC'TnA_GC

Cuts at:      0     218     474 
   Size:        218     256
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     379     474 
   Size:        379      95
 
BsmI GAATG_Cn'

Cuts at:      0     335     474 
   Size:        335     139
 
BstBI TT'CG_AA

Cuts at:      0     450     474 
   Size:        450      24
 
Bsu36I CC'TnA_GG

Cuts at:      0     362     474 
   Size:        362     112
 
BtsI GCAGTG_nn'

Cuts at:      0     177     212     474 
   Size:        177      35     262

  Fragments arranged by size: 

                 262     177      35
 
EaeI y'GGCC_r

Cuts at:      0     251     351     474 
   Size:        251     100     123

  Fragments arranged by size: 

                 251     123     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     319     474 
   Size:        185     134     155

  Fragments arranged by size: 

                 185     155     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     222     474 
   Size:         45     177     252

  Fragments arranged by size: 

                 252     177      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     434     474 
   Size:        434      40
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     474 
   Size:        407      67
 
MscI TGG'CCA

Cuts at:      0     253     353     474 
   Size:        253     100     121

  Fragments arranged by size: 

                 253     121     100
 
PpuMI rG'GwC_Cy

Cuts at:      0      45     222     474 
   Size:         45     177     252

  Fragments arranged by size: 

                 252     177      45
 
ScaI AGT'ACT

Cuts at:      0     296     474 
   Size:        296     178
 
SmlI C'TyrA_G

Cuts at:      0      70     474 
   Size:         70     404
 
SspI AAT'ATT

Cuts at:      0     113     474 
   Size:        113     361
 
StyI C'CwwG_G

Cuts at:      0     225     474 
   Size:        225     249
 
TatI w'GTAC_w

Cuts at:      0     294     474 
   Size:        294     180
 

 Enzymes that do cut:

    AflII     AloI    AvrII    BbvCI    BmgBI     BpmI   Bpu10I    BseRI
     BsmI    BstBI   Bsu36I     BtsI     EaeI  Eco57MI EcoO109I     FalI
     MmeI     MscI    PpuMI     ScaI     SmlI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI