Restriction map for
pFN21AE1336
(Linear) (Six-base) MAPSORT of: KIEE1336.seq Check: 1190 from: 1 to: 750
KIEE1336.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 750
Size: 323 427
AatII G_ACGT'C
Cuts at: 0 56 750
Size: 56 694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 750
Size: 181 569
AleI CACnn'nnGTG
Cuts at: 0 292 750
Size: 292 458
ApaI G_GGCC'C
Cuts at: 0 158 750
Size: 158 592
ApoI r'AATT_y
Cuts at: 0 373 750
Size: 373 377
BanI G'GyrC_C
Cuts at: 0 294 750
Size: 294 456
BanII G_rGCy'C
Cuts at: 0 158 248 750
Size: 158 90 502
Fragments arranged by size:
502 158 90
Bme1580I G_kGCm'C
Cuts at: 0 158 299 617 750
Size: 158 141 318 133
Fragments arranged by size:
318 158 141 133
BmtI G_CTAG'C
Cuts at: 0 424 750
Size: 424 326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 750
Size: 577 173
BsaHI Gr'CG_yC
Cuts at: 0 53 76 750
Size: 53 23 674
Fragments arranged by size:
674 53 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 750
Size: 331 30 389
Fragments arranged by size:
389 331 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 750
Size: 379 371
BseYI C'CCAG_C
Cuts at: 0 575 656 750
Size: 575 81 94
Fragments arranged by size:
575 94 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 750
Size: 91 659
BsiHKAI G_wGCw'C
Cuts at: 0 248 750
Size: 248 502
Bsp1286I G_dGCh'C
Cuts at: 0 158 248 299 617 750
Size: 158 90 51 318 133
Fragments arranged by size:
318 158 133 90 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 323 750
Size: 155 168 427
Fragments arranged by size:
427 168 155
BsrDI GCAATG_nn'
Cuts at: 0 542 750
Size: 542 208
BssSI C'ACGA_G
Cuts at: 0 604 750
Size: 604 146
BstAPI GCAn_nnn'nTGC
Cuts at: 0 561 750
Size: 561 189
BstEII G'GTnAC_C
Cuts at: 0 621 750
Size: 621 129
BstYI r'GATC_y
Cuts at: 0 275 750
Size: 275 475
Bsu36I CC'TnA_GG
Cuts at: 0 730 750
Size: 730 20
BtgI C'CryG_G
Cuts at: 0 59 546 750
Size: 59 487 204
Fragments arranged by size:
487 204 59
EarI CTCTTCn'nnn_
Cuts at: 0 253 356 443 750
Size: 253 103 87 307
Fragments arranged by size:
307 253 103 87
EcoICRI GAG'CTC
Cuts at: 0 246 750
Size: 246 504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 577 750
Size: 181 396 173
Fragments arranged by size:
396 181 173
EcoO109I rG'GnC_Cy
Cuts at: 0 154 155 750
Size: 154 1 595
Fragments arranged by size:
595 154 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 573 605 750
Size: 123 32 418 32 145
Fragments arranged by size:
418 145 123 32 32
HincII GTy'rAC
Cuts at: 0 123 750
Size: 123 627
HpaI GTT'AAC
Cuts at: 0 123 750
Size: 123 627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 750
Size: 380 370
MslI CAynn'nnrTG
Cuts at: 0 292 523 608 750
Size: 292 231 85 142
Fragments arranged by size:
292 231 142 85
MspA1I CmG'CkG
Cuts at: 0 36 548 579 750
Size: 36 512 31 171
Fragments arranged by size:
512 171 36 31
NheI G'CTAG_C
Cuts at: 0 420 750
Size: 420 330
NspI r_CATG'y
Cuts at: 0 555 750
Size: 555 195
PasI CC'CwG_GG
Cuts at: 0 502 617 750
Size: 502 115 133
Fragments arranged by size:
502 133 115
PfoI T'CCnGG_A
Cuts at: 0 239 750
Size: 239 511
PshAI GACnn'nnGTC
Cuts at: 0 436 750
Size: 436 314
PspOMI G'GGCC_C
Cuts at: 0 154 750
Size: 154 596
PstI C_TGCA'G
Cuts at: 0 491 750
Size: 491 259
SacI G_AGCT'C
Cuts at: 0 248 750
Size: 248 502
SacII CC_GC'GG
Cuts at: 0 549 750
Size: 549 201
SapI GCTCTTCn'nnn_
Cuts at: 0 253 750
Size: 253 497
SfcI C'TryA_G
Cuts at: 0 487 750
Size: 487 263
SphI G_CATG'C
Cuts at: 0 555 750
Size: 555 195
StuI AGG'CCT
Cuts at: 0 391 750
Size: 391 359
StyI C'CwwG_G
Cuts at: 0 641 750
Size: 641 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 331 363 750
Size: 331 32 387
Fragments arranged by size:
387 331 32
Tth111I GACn'n_nGTC
Cuts at: 0 6 750
Size: 6 744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 750
Size: 441 309
ZraI GAC'GTC
Cuts at: 0 54 750
Size: 54 696
Enzymes that do cut:
AarI AatII AcuI AleI ApaI ApoI BanI BanII
Bme1580I BmtI BpmI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII BstYI
Bsu36I BtgI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII
HpaI MmeI MslI MspA1I NheI NspI PasI PfoI
PshAI PspOMI PstI SacI SacII SapI SfcI SphI
StuI StyI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI
TaqII TatI XbaI XhoI XmaI XmnI