Restriction map for
pFN21AE1335
(Linear) (Six-base) MAPSORT of: KIEE1335.seq Check: 4205 from: 1 to: 696
KIEE1335.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 440 696
Size: 440 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 493 696
Size: 475 18 203
Fragments arranged by size:
475 203 18
AflII C'TTAA_G
Cuts at: 0 505 696
Size: 505 191
AlwNI CAG_nnn'CTG
Cuts at: 0 23 261 351 678 696
Size: 23 238 90 327 18
Fragments arranged by size:
327 238 90 23 18
ApaI G_GGCC'C
Cuts at: 0 117 380 685 696
Size: 117 263 305 11
Fragments arranged by size:
305 263 117 11
BanI G'GyrC_C
Cuts at: 0 104 696
Size: 104 592
BanII G_rGCy'C
Cuts at: 0 117 144 366 380 685 696
Size: 117 27 222 14 305 11
Fragments arranged by size:
305 222 117 27 14 11
BbeI G_GCGC'C
Cuts at: 0 108 696
Size: 108 588
BbsI GAAGACnn'nnnn_
Cuts at: 0 641 696
Size: 641 55
BglI GCCn_nnn'nGGC
Cuts at: 0 375 696
Size: 375 321
Bme1580I G_kGCm'C
Cuts at: 0 117 380 685 696
Size: 117 263 305 11
Fragments arranged by size:
305 263 117 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 679 696
Size: 679 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 421 658 696
Size: 289 132 237 38
Fragments arranged by size:
289 237 132 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 514 696
Size: 514 182
BsaHI Gr'CG_yC
Cuts at: 0 105 696
Size: 105 591
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 112 142 597 627 631 661
Size: 55 30 27 30 455 30 4 30
Cuts at: 661 696
Size: 35
Fragments arranged by size:
455 55 35 30 30 30 30 27
4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 169 696
Size: 24 145 527
Fragments arranged by size:
527 145 24
BseYI C'CCAG_C
Cuts at: 0 96 657 696
Size: 96 561 39
Fragments arranged by size:
561 96 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 696
Size: 150 546
BsmI GAATG_Cn'
Cuts at: 0 393 447 696
Size: 393 54 249
Fragments arranged by size:
393 249 54
Bsp1286I G_dGCh'C
Cuts at: 0 117 144 366 380 685 696
Size: 117 27 222 14 305 11
Fragments arranged by size:
305 222 117 27 14 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 284 440 696
Size: 269 15 156 256
Fragments arranged by size:
269 256 156 15
BsrFI r'CCGG_y
Cuts at: 0 101 227 696
Size: 101 126 469
Fragments arranged by size:
469 126 101
BsrGI T'GTAC_A
Cuts at: 0 146 696
Size: 146 550
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 696
Size: 351 345
BstYI r'GATC_y
Cuts at: 0 675 696
Size: 675 21
BtgI C'CryG_G
Cuts at: 0 135 356 515 696
Size: 135 221 159 181
Fragments arranged by size:
221 181 159 135
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 696
Size: 535 161
EaeI y'GGCC_r
Cuts at: 0 186 326 696
Size: 186 140 370
Fragments arranged by size:
370 186 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 421 475 493 658 696
Size: 289 132 54 18 165 38
Fragments arranged by size:
289 165 132 54 38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 696
Size: 370 326
EcoO109I rG'GnC_Cy
Cuts at: 0 113 114 376 642 696
Size: 113 1 262 266 54
Fragments arranged by size:
266 262 113 54 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 498 696
Size: 498 198
FspI TGC'GCA
Cuts at: 0 280 322 696
Size: 280 42 374
Fragments arranged by size:
374 280 42
FspAI rTGC'GCAy
Cuts at: 0 322 696
Size: 322 374
HaeII r_GCGC'y
Cuts at: 0 108 352 696
Size: 108 244 344
Fragments arranged by size:
344 244 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 631 663 696
Size: 55 32 544 32 33
Fragments arranged by size:
544 55 33 32 32
HindIII A'AGCT_T
Cuts at: 0 286 696
Size: 286 410
KasI G'GCGC_C
Cuts at: 0 104 696
Size: 104 592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 696
Size: 98 598
MscI TGG'CCA
Cuts at: 0 188 328 696
Size: 188 140 368
Fragments arranged by size:
368 188 140
MslI CAynn'nnrTG
Cuts at: 0 166 696
Size: 166 530
MspA1I CmG'CkG
Cuts at: 0 137 267 336 696
Size: 137 130 69 360
Fragments arranged by size:
360 137 130 69
NaeI GCC'GGC
Cuts at: 0 103 229 696
Size: 103 126 467
Fragments arranged by size:
467 126 103
NarI GG'CG_CC
Cuts at: 0 105 696
Size: 105 591
NcoI C'CATG_G
Cuts at: 0 356 515 696
Size: 356 159 181
Fragments arranged by size:
356 181 159
NgoMIV G'CCGG_C
Cuts at: 0 101 227 696
Size: 101 126 469
Fragments arranged by size:
469 126 101
PasI CC'CwG_GG
Cuts at: 0 109 372 696
Size: 109 263 324
Fragments arranged by size:
324 263 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 530 696
Size: 530 166
PfoI T'CCnGG_A
Cuts at: 0 485 696
Size: 485 211
PpuMI rG'GwC_Cy
Cuts at: 0 642 696
Size: 642 54
PspOMI G'GGCC_C
Cuts at: 0 113 376 681 696
Size: 113 263 305 15
Fragments arranged by size:
305 263 113 15
PstI C_TGCA'G
Cuts at: 0 266 696
Size: 266 430
PvuII CAG'CTG
Cuts at: 0 267 696
Size: 267 429
SacII CC_GC'GG
Cuts at: 0 138 696
Size: 138 558
SfcI C'TryA_G
Cuts at: 0 262 696
Size: 262 434
SfoI GGC'GCC
Cuts at: 0 106 696
Size: 106 590
SmlI C'TyrA_G
Cuts at: 0 505 696
Size: 505 191
StyI C'CwwG_G
Cuts at: 0 356 515 590 696
Size: 356 159 75 106
Fragments arranged by size:
356 159 106 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 649 696
Size: 649 47
TatI w'GTAC_w
Cuts at: 0 146 696
Size: 146 550
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 144 597 629 696
Size: 112 32 453 32 67
Fragments arranged by size:
453 112 67 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 696
Size: 675 21
Enzymes that do cut:
AarI AcuI AflII AlwNI ApaI BanI BanII BbeI
BbsI BglI Bme1580I BmrI BpmI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrFI BsrGI
BstAPI BstYI BtgI DrdI EaeI Eco57MI EcoNI EcoO109I
FalI FspI FspAI HaeII Hin4I HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SfcI
SfoI SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EarI EciI EcoICRI EcoRI EcoRV FseI HincII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI