Restriction map for
pFN21AE1334
(Linear) (Six-base) MAPSORT of: KIEE1334.seq Check: 800 from: 1 to: 1131
KIEE1334.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 386 757 1131
Size: 170 216 371 374
Fragments arranged by size:
374 371 216 170
AfeI AGC'GCT
Cuts at: 0 560 1131
Size: 560 571
AflII C'TTAA_G
Cuts at: 0 31 1131
Size: 31 1100
AflIII A'CryG_T
Cuts at: 0 929 1131
Size: 929 202
ApoI r'AATT_y
Cuts at: 0 337 1131
Size: 337 794
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 607 640 1131
Size: 607 33 491
Fragments arranged by size:
607 491 33
BanII G_rGCy'C
Cuts at: 0 958 1131
Size: 958 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 1131
Size: 158 973
BbvCI CC'TCA_GC
Cuts at: 0 562 1131
Size: 562 569
BglII A'GATC_T
Cuts at: 0 371 1131
Size: 371 760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 797 877 957 1131
Size: 658 139 80 80 174
Fragments arranged by size:
658 174 139 80 80
Bpu10I CC'TnA_GC
Cuts at: 0 562 1131
Size: 562 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 517 1131
Size: 301 216 614
Fragments arranged by size:
614 301 216
BsaAI yAC'GTr
Cuts at: 0 885 1131
Size: 885 246
BsiHKAI G_wGCw'C
Cuts at: 0 590 1131
Size: 590 541
Bsp1286I G_dGCh'C
Cuts at: 0 590 958 1131
Size: 590 368 173
Fragments arranged by size:
590 368 173
BspHI T'CATG_A
Cuts at: 0 330 1131
Size: 330 801
BsrDI GCAATG_nn'
Cuts at: 0 899 1131
Size: 899 232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 1131
Size: 549 582
BstYI r'GATC_y
Cuts at: 0 371 1131
Size: 371 760
BtgI C'CryG_G
Cuts at: 0 786 1131
Size: 786 345
DraIII CAC_nnn'GTG
Cuts at: 0 930 1131
Size: 930 201
EarI CTCTTCn'nnn_
Cuts at: 0 110 362 521 840 918 1131
Size: 110 252 159 319 78 213
Fragments arranged by size:
319 252 213 159 110 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 386 658 757 797 877 957 1131
Size: 170 216 272 99 40 80 80 174
Fragments arranged by size:
272 216 174 170 99 80 80 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 293 1131
Size: 293 838
EcoRI G'AATT_C
Cuts at: 0 337 1131
Size: 337 794
HaeII r_GCGC'y
Cuts at: 0 562 1131
Size: 562 569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 538 570 577 609 1131
Size: 229 32 277 32 7 32 522
Fragments arranged by size:
522 277 229 32 32 32 7
HindIII A'AGCT_T
Cuts at: 0 319 1131
Size: 319 812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 1131
Size: 20 1111
MslI CAynn'nnrTG
Cuts at: 0 481 1131
Size: 481 650
NdeI CA'TA_TG
Cuts at: 0 421 1131
Size: 421 710
NspI r_CATG'y
Cuts at: 0 986 1131
Size: 986 145
PasI CC'CwG_GG
Cuts at: 0 48 1131
Size: 48 1083
PfoI T'CCnGG_A
Cuts at: 0 635 934 1131
Size: 635 299 197
Fragments arranged by size:
635 299 197
PmlI CAC'GTG
Cuts at: 0 885 1131
Size: 885 246
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 83 115 1131
Size: 83 32 1016
Fragments arranged by size:
1016 83 32
PstI C_TGCA'G
Cuts at: 0 775 1131
Size: 775 356
SapI GCTCTTCn'nnn_
Cuts at: 0 918 1131
Size: 918 213
SfcI C'TryA_G
Cuts at: 0 305 510 771 1131
Size: 305 205 261 360
Fragments arranged by size:
360 305 261 205
SmlI C'TyrA_G
Cuts at: 0 31 316 496 1131
Size: 31 285 180 635
Fragments arranged by size:
635 285 180 31
SspI AAT'ATT
Cuts at: 0 133 997 1131
Size: 133 864 134
Fragments arranged by size:
864 134 133
StuI AGG'CCT
Cuts at: 0 769 1131
Size: 769 362
StyI C'CwwG_G
Cuts at: 0 749 821 1057 1131
Size: 749 72 236 74
Fragments arranged by size:
749 236 74 72
TatI w'GTAC_w
Cuts at: 0 99 1131
Size: 99 1032
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 916 948 1131
Size: 916 32 183
Fragments arranged by size:
916 183 32
XmnI GAAnn'nnTTC
Cuts at: 0 895 1025 1131
Size: 895 130 106
Fragments arranged by size:
895 130 106
Enzymes that do cut:
AcuI AfeI AflII AflIII ApoI BaeI BanII BbsI
BbvCI BglII BpmI Bpu10I BpuEI BsaAI BsiHKAI Bsp1286I
BspHI BsrDI BstXI BstYI BtgI DraIII EarI Eco57MI
EcoNI EcoRI HaeII Hin4I HindIII MmeI MslI NdeI
NspI PasI PfoI PmlI PsrI PstI SapI SfcI
SmlI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI