Restriction map for
pFN21AE1333
(Linear) (Six-base) MAPSORT of: KIEE1333.seq Check: 7885 from: 1 to: 1791
KIEE1333.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 247 1791
Size: 247 1544
Acc65I G'GTAC_C
Cuts at: 0 986 1592 1791
Size: 986 606 199
Fragments arranged by size:
986 606 199
AleI CACnn'nnGTG
Cuts at: 0 560 787 1791
Size: 560 227 1004
Fragments arranged by size:
1004 560 227
AlwNI CAG_nnn'CTG
Cuts at: 0 1589 1791
Size: 1589 202
ApoI r'AATT_y
Cuts at: 0 409 570 1050 1791
Size: 409 161 480 741
Fragments arranged by size:
741 480 409 161
AseI AT'TA_AT
Cuts at: 0 899 1791
Size: 899 892
AvrII C'CTAG_G
Cuts at: 0 940 1791
Size: 940 851
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 978 1011 1791
Size: 978 33 780
Fragments arranged by size:
978 780 33
BanI G'GyrC_C
Cuts at: 0 986 1592 1602 1791
Size: 986 606 10 189
Fragments arranged by size:
986 606 189 10
BanII G_rGCy'C
Cuts at: 0 30 887 1791
Size: 30 857 904
Fragments arranged by size:
904 857 30
BglI GCCn_nnn'nGGC
Cuts at: 0 248 1791
Size: 248 1543
Bme1580I G_kGCm'C
Cuts at: 0 1607 1791
Size: 1607 184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 512 1791
Size: 512 1279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 507 518 1409 1726 1791
Size: 51 456 11 891 317 65
Fragments arranged by size:
891 456 317 65 51 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 675 753 1195 1615 1791
Size: 675 78 442 420 176
Fragments arranged by size:
675 442 420 176 78
BsaAI yAC'GTr
Cuts at: 0 358 1241 1791
Size: 358 883 550
Fragments arranged by size:
883 550 358
BsaBI GATnn'nnATC
Cuts at: 0 1493 1791
Size: 1493 298
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 491 521 860 1791
Size: 491 30 339 931
Fragments arranged by size:
931 491 339 30
BsiHKAI G_wGCw'C
Cuts at: 0 1649 1791
Size: 1649 142
Bsp1286I G_dGCh'C
Cuts at: 0 30 887 1607 1649 1791
Size: 30 857 720 42 142
Fragments arranged by size:
857 720 142 42 30
BspHI T'CATG_A
Cuts at: 0 1197 1791
Size: 1197 594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 247 1791
Size: 247 1544
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1234 1791
Size: 1234 557
BstYI r'GATC_y
Cuts at: 0 259 1791
Size: 259 1532
BtgI C'CryG_G
Cuts at: 0 1299 1791
Size: 1299 492
BtsI GCAGTG_nn'
Cuts at: 0 834 1675 1791
Size: 834 841 116
Fragments arranged by size:
841 834 116
DraIII CAC_nnn'GTG
Cuts at: 0 508 1791
Size: 508 1283
EarI CTCTTCn'nnn_
Cuts at: 0 35 103 1337 1791
Size: 35 68 1234 454
Fragments arranged by size:
1234 454 68 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 507 518 1409 1726 1791
Size: 51 456 11 891 317 65
Fragments arranged by size:
891 456 317 65 51 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 66 1791
Size: 66 1725
EcoRI G'AATT_C
Cuts at: 0 409 1791
Size: 409 1382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1586 1791
Size: 1586 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 1791
Size: 105 32 1654
Fragments arranged by size:
1654 105 32
HincII GTy'rAC
Cuts at: 0 197 1791
Size: 197 1594
KpnI G_GTAC'C
Cuts at: 0 990 1596 1791
Size: 990 606 195
Fragments arranged by size:
990 606 195
MfeI C'AATT_G
Cuts at: 0 144 1791
Size: 144 1647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1362 1432 1791
Size: 1362 70 359
Fragments arranged by size:
1362 359 70
MslI CAynn'nnrTG
Cuts at: 0 560 787 842 1191 1260 1791
Size: 560 227 55 349 69 531
Fragments arranged by size:
560 531 349 227 69 55
MspA1I CmG'CkG
Cuts at: 0 416 560 605 992 1791
Size: 416 144 45 387 799
Fragments arranged by size:
799 416 387 144 45
NcoI C'CATG_G
Cuts at: 0 1299 1791
Size: 1299 492
NspI r_CATG'y
Cuts at: 0 922 1791
Size: 922 869
PflMI CCAn_nnn'nTGG
Cuts at: 0 74 1264 1791
Size: 74 1190 527
Fragments arranged by size:
1190 527 74
PmlI CAC'GTG
Cuts at: 0 1241 1791
Size: 1241 550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1631 1663 1791
Size: 1631 32 128
Fragments arranged by size:
1631 128 32
PsiI TTA'TAA
Cuts at: 0 1310 1791
Size: 1310 481
PstI C_TGCA'G
Cuts at: 0 1405 1791
Size: 1405 386
PvuII CAG'CTG
Cuts at: 0 416 605 1791
Size: 416 189 1186
Fragments arranged by size:
1186 416 189
SapI GCTCTTCn'nnn_
Cuts at: 0 35 1791
Size: 35 1756
SexAI A'CCwGG_T
Cuts at: 0 1588 1791
Size: 1588 203
SfcI C'TryA_G
Cuts at: 0 600 1016 1401 1747 1791
Size: 600 416 385 346 44
Fragments arranged by size:
600 416 385 346 44
SnaBI TAC'GTA
Cuts at: 0 358 1791
Size: 358 1433
SpeI A'CTAG_T
Cuts at: 0 451 1791
Size: 451 1340
StyI C'CwwG_G
Cuts at: 0 940 1071 1227 1299 1791
Size: 940 131 156 72 492
Fragments arranged by size:
940 492 156 131 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 294 359 1791
Size: 174 120 65 1432
Fragments arranged by size:
1432 174 120 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1791
Size: 1230 32 529
Fragments arranged by size:
1230 529 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 923 1534 1791
Size: 923 611 257
Fragments arranged by size:
923 611 257
Enzymes that do cut:
AarI Acc65I AleI AlwNI ApoI AseI AvrII BaeI
BanI BanII BglI Bme1580I BplI BpmI BsaI BsaAI
BsaBI BseRI BsiHKAI Bsp1286I BspHI BspMI BstXI BstYI
BtgI BtsI DraIII EarI Eco57MI EcoNI EcoRI FalI
Hin4I HincII KpnI MfeI MmeI MslI MspA1I NcoI
NspI PflMI PmlI PpiI PsiI PstI PvuII SapI
SexAI SfcI SnaBI SpeI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI