Restriction map for
pFN21AE1331
(Linear) (Six-base) MAPSORT of: KIEE1331.seq Check: 5395 from: 1 to: 1008
KIEE1331.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 628 712 986 1008
Size: 309 319 84 274 22
Fragments arranged by size:
319 309 274 84 22
AflIII A'CryG_T
Cuts at: 0 823 1008
Size: 823 185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 961 1008
Size: 352 609 47
Fragments arranged by size:
609 352 47
AlwNI CAG_nnn'CTG
Cuts at: 0 843 1008
Size: 843 165
ApoI r'AATT_y
Cuts at: 0 78 515 1008
Size: 78 437 493
Fragments arranged by size:
493 437 78
BanI G'GyrC_C
Cuts at: 0 124 336 443 1008
Size: 124 212 107 565
Fragments arranged by size:
565 212 124 107
BanII G_rGCy'C
Cuts at: 0 29 506 1008
Size: 29 477 502
Fragments arranged by size:
502 477 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 226 1008
Size: 226 782
BglI GCCn_nnn'nGGC
Cuts at: 0 838 1008
Size: 838 170
BlpI GC'TnA_GC
Cuts at: 0 414 1008
Size: 414 594
Bme1580I G_kGCm'C
Cuts at: 0 321 341 1008
Size: 321 20 667
Fragments arranged by size:
667 321 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 478 1008
Size: 478 530
BmtI G_CTAG'C
Cuts at: 0 227 1008
Size: 227 781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 1008
Size: 33 975
BsaBI GATnn'nnATC
Cuts at: 0 730 1008
Size: 730 278
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1008
Size: 898 110
BseYI C'CCAG_C
Cuts at: 0 833 1008
Size: 833 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 494 567 1008
Size: 164 330 73 441
Fragments arranged by size:
441 330 164 73
BsiHKAI G_wGCw'C
Cuts at: 0 29 1008
Size: 29 979
Bsp1286I G_dGCh'C
Cuts at: 0 29 321 341 506 1008
Size: 29 292 20 165 502
Fragments arranged by size:
502 292 165 29 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 857 1008
Size: 173 684 151
Fragments arranged by size:
684 173 151
BstBI TT'CG_AA
Cuts at: 0 65 1008
Size: 65 943
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 722 1008
Size: 210 512 286
Fragments arranged by size:
512 286 210
BtsI GCAGTG_nn'
Cuts at: 0 181 321 541 1008
Size: 181 140 220 467
Fragments arranged by size:
467 220 181 140
DraIII CAC_nnn'GTG
Cuts at: 0 891 1008
Size: 891 117
EaeI y'GGCC_r
Cuts at: 0 557 1008
Size: 557 451
EarI CTCTTCn'nnn_
Cuts at: 0 244 265 328 1008
Size: 244 21 63 680
Fragments arranged by size:
680 244 63 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1008
Size: 37 971
EcoICRI GAG'CTC
Cuts at: 0 27 1008
Size: 27 981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 628 712 986 1008
Size: 309 319 84 274 22
Fragments arranged by size:
319 309 274 84 22
EcoO109I rG'GnC_Cy
Cuts at: 0 483 718 1008
Size: 483 235 290
Fragments arranged by size:
483 290 235
EcoRI G'AATT_C
Cuts at: 0 78 1008
Size: 78 930
HincII GTy'rAC
Cuts at: 0 821 1008
Size: 821 187
MscI TGG'CCA
Cuts at: 0 559 1008
Size: 559 449
MslI CAynn'nnrTG
Cuts at: 0 334 1008
Size: 334 674
MspA1I CmG'CkG
Cuts at: 0 57 142 266 303 1008
Size: 57 85 124 37 705
Fragments arranged by size:
705 124 85 57 37
NheI G'CTAG_C
Cuts at: 0 223 1008
Size: 223 785
NspI r_CATG'y
Cuts at: 0 827 1008
Size: 827 181
PasI CC'CwG_GG
Cuts at: 0 352 456 498 1008
Size: 352 104 42 510
Fragments arranged by size:
510 352 104 42
PciI A'CATG_T
Cuts at: 0 823 1008
Size: 823 185
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 1008
Size: 468 540
PpuMI rG'GwC_Cy
Cuts at: 0 483 1008
Size: 483 525
PvuII CAG'CTG
Cuts at: 0 57 142 303 1008
Size: 57 85 161 705
Fragments arranged by size:
705 161 85 57
SacI G_AGCT'C
Cuts at: 0 29 1008
Size: 29 979
SfcI C'TryA_G
Cuts at: 0 632 1008
Size: 632 376
SmlI C'TyrA_G
Cuts at: 0 48 1008
Size: 48 960
StuI AGG'CCT
Cuts at: 0 491 1008
Size: 491 517
StyI C'CwwG_G
Cuts at: 0 683 993 1008
Size: 683 310 15
Fragments arranged by size:
683 310 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 1008
Size: 460 548
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI BanI BanII BciVI
BglI BlpI Bme1580I BmrI BmtI BpuEI BsaBI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BstBI BstXI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
HincII MscI MslI MspA1I NheI NspI PasI PciI
PflMI PpuMI PvuII SacI SfcI SmlI StuI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BclI BfrBI BglII BmgBI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EagI EcoNI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI