(Linear) (Six-base) MAPSORT of: KIEE1330.seq  Check: 7062  from: 1  to: 396



KIEE1330.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AatII G_ACGT'C

Cuts at:      0      27     396 
   Size:         27     369
 
AccI GT'mk_AC

Cuts at:      0     149     396 
   Size:        149     247
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     396 
   Size:        150     246
 
AhdI GACnn_n'nnGTC

Cuts at:      0      99     396 
   Size:         99     297
 
ApoI r'AATT_y

Cuts at:      0     253     396 
   Size:        253     143
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     185     396 
   Size:        185     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     342     396 
   Size:         43     299      54

  Fragments arranged by size: 

                 299      54      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      40     396 
   Size:         40     356
 
BsaHI Gr'CG_yC

Cuts at:      0      24     396 
   Size:         24     372
 
BseYI C'CCAG_C

Cuts at:      0     316     396 
   Size:        316      80
 
BstYI r'GATC_y

Cuts at:      0       3     396 
   Size:          3     393
 
BtgI C'CryG_G

Cuts at:      0      18     396 
   Size:         18     378
 
DraI TTT'AAA

Cuts at:      0     138     293     396 
   Size:        138     155     103

  Fragments arranged by size: 

                 155     138     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     396 
   Size:        150     246
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      85     117     396 
   Size:         85      32     279

  Fragments arranged by size: 

                 279      85      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     396 
   Size:        329      67
 
NcoI C'CATG_G

Cuts at:      0      18     396 
   Size:         18     378
 
PstI C_TGCA'G

Cuts at:      0     204     396 
   Size:        204     192
 
SfcI C'TryA_G

Cuts at:      0     200     396 
   Size:        200     196
 
SmlI C'TyrA_G

Cuts at:      0      58     321     396 
   Size:         58     263      75

  Fragments arranged by size: 

                 263      75      58
 
SspI AAT'ATT

Cuts at:      0     144     396 
   Size:        144     252
 
StyI C'CwwG_G

Cuts at:      0      18     396 
   Size:         18     378
 
ZraI GAC'GTC

Cuts at:      0      25     396 
   Size:         25     371
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AhdI     ApoI    BciVI    BpuEI     BsaI
    BsaHI    BseYI    BstYI     BtgI     DraI  Eco57MI    Hin4I     MmeI
     NcoI     PstI     SfcI     SmlI     SspI     StyI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI