(Linear) (Six-base) MAPSORT of: KIEE1323.seq  Check: 3250  from: 1  to: 285



KIEE1323.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

ApoI r'AATT_y

Cuts at:      0     127     285 
   Size:        127     158
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      94     285 
   Size:         94     191
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     285 
   Size:        250      35
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     262     285 
   Size:        262      23
 
EaeI y'GGCC_r

Cuts at:      0     155     285 
   Size:        155     130
 
FspI TGC'GCA

Cuts at:      0     219     285 
   Size:        219      66
 
MscI TGG'CCA

Cuts at:      0     157     285 
   Size:        157     128
 
MspA1I CmG'CkG

Cuts at:      0     153     285 
   Size:        153     132
 
PshAI GACnn'nnGTC

Cuts at:      0      92     285 
   Size:         92     193
 
PvuII CAG'CTG

Cuts at:      0     153     285 
   Size:        153     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     228     285 
   Size:        228      57
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     233     285 
   Size:        233      52
 

 Enzymes that do cut:

     ApoI    BciVI    BseRI    BsmBI     EaeI     FspI     MscI   MspA1I
    PshAI    PvuII    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI