Restriction map for
pFN21AE1322
(Linear) (Six-base) MAPSORT of: KIEE1322.seq Check: 1020 from: 1 to: 654
KIEE1322.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AatII G_ACGT'C
Cuts at: 0 320 654
Size: 320 334
AccI GT'mk_AC
Cuts at: 0 97 654
Size: 97 557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 562 654
Size: 388 174 92
Fragments arranged by size:
388 174 92
AleI CACnn'nnGTG
Cuts at: 0 631 654
Size: 631 23
AlwNI CAG_nnn'CTG
Cuts at: 0 138 341 423 654
Size: 138 203 82 231
Fragments arranged by size:
231 203 138 82
ApoI r'AATT_y
Cuts at: 0 148 654
Size: 148 506
BanII G_rGCy'C
Cuts at: 0 201 515 654
Size: 201 314 139
Fragments arranged by size:
314 201 139
BglII A'GATC_T
Cuts at: 0 578 654
Size: 578 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 30 654
Size: 22 8 624
Fragments arranged by size:
624 22 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 654
Size: 597 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 654
Size: 60 594
BsaHI Gr'CG_yC
Cuts at: 0 317 527 654
Size: 317 210 127
Fragments arranged by size:
317 210 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 654
Size: 538 30 86
Fragments arranged by size:
538 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 654
Size: 289 365
BseYI C'CCAG_C
Cuts at: 0 156 654
Size: 156 498
BsmBI CGTCTCn'nnnn_
Cuts at: 0 324 654
Size: 324 330
Bsp1286I G_dGCh'C
Cuts at: 0 201 515 654
Size: 201 314 139
Fragments arranged by size:
314 201 139
BsrDI GCAATG_nn'
Cuts at: 0 236 654
Size: 236 418
BsrGI T'GTAC_A
Cuts at: 0 623 654
Size: 623 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 330 522 654
Size: 12 318 192 132
Fragments arranged by size:
318 192 132 12
BstYI r'GATC_y
Cuts at: 0 578 654
Size: 578 76
BstZ17I GTA'TAC
Cuts at: 0 98 654
Size: 98 556
BtgI C'CryG_G
Cuts at: 0 30 436 654
Size: 30 406 218
Fragments arranged by size:
406 218 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 240 275 654
Size: 240 35 379
Fragments arranged by size:
379 240 35
EaeI y'GGCC_r
Cuts at: 0 332 654
Size: 332 322
EarI CTCTTCn'nnn_
Cuts at: 0 266 424 654
Size: 266 158 230
Fragments arranged by size:
266 230 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 654
Size: 40 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 562 654
Size: 388 174 92
Fragments arranged by size:
388 174 92
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 654
Size: 477 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 440 472 538 570 654
Size: 440 32 66 32 84
Fragments arranged by size:
440 84 66 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 654
Size: 426 228
MscI TGG'CCA
Cuts at: 0 334 654
Size: 334 320
MslI CAynn'nnrTG
Cuts at: 0 631 654
Size: 631 23
MspA1I CmG'CkG
Cuts at: 0 32 330 654
Size: 32 298 324
Fragments arranged by size:
324 298 32
NcoI C'CATG_G
Cuts at: 0 436 654
Size: 436 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 654
Size: 312 342
PfoI T'CCnGG_A
Cuts at: 0 59 654
Size: 59 595
PvuII CAG'CTG
Cuts at: 0 330 654
Size: 330 324
SacII CC_GC'GG
Cuts at: 0 33 654
Size: 33 621
SfcI C'TryA_G
Cuts at: 0 345 654
Size: 345 309
SmlI C'TyrA_G
Cuts at: 0 75 654
Size: 75 579
StyI C'CwwG_G
Cuts at: 0 436 654
Size: 436 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 654
Size: 226 428
TatI w'GTAC_w
Cuts at: 0 623 654
Size: 623 31
XmnI GAAnn'nnTTC
Cuts at: 0 547 654
Size: 547 107
ZraI GAC'GTC
Cuts at: 0 318 654
Size: 318 336
Enzymes that do cut:
AatII AccI AcuI AleI AlwNI ApoI BanII BglII
BmrI BplI BpuEI BsaHI BsaXI BseRI BseYI BsmBI
Bsp1286I BsrDI BsrGI BstXI BstYI BstZ17I BtgI CspCI
EaeI EarI EciI Eco57MI FalI Hin4I MmeI MscI
MslI MspA1I NcoI PflMI PfoI PvuII SacII SfcI
SmlI StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI
ClaI DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI