(Linear) (Six-base) MAPSORT of: KIEE1321.seq  Check: 5940  from: 1  to: 321



KIEE1321.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     144     321 
   Size:        144     177
 
ApoI r'AATT_y

Cuts at:      0     152     321 
   Size:        152     169
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     217     321 
   Size:        145      72     104

  Fragments arranged by size: 

                 145     104      72
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      92     122     321 
   Size:         92      30     199

  Fragments arranged by size: 

                 199      92      30
 
BseYI C'CCAG_C

Cuts at:      0      28     321 
   Size:         28     293
 
BsiHKAI G_wGCw'C

Cuts at:      0     316     321 
   Size:        316       5
 
Bsp1286I G_dGCh'C

Cuts at:      0     316     321 
   Size:        316       5
 
BsrFI r'CCGG_y

Cuts at:      0      86     321 
   Size:         86     235
 
BssHII G'CGCG_C

Cuts at:      0      90     321 
   Size:         90     231
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     144     321 
   Size:        144     177
 
BtgI C'CryG_G

Cuts at:      0     107     272     321 
   Size:        107     165      49

  Fragments arranged by size: 

                 165     107      49
 
BtsI GCAGTG_nn'

Cuts at:      0     142     201     321 
   Size:        142      59     120

  Fragments arranged by size: 

                 142     120      59
 
EaeI y'GGCC_r

Cuts at:      0      77     321 
   Size:         77     244
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       9     321 
   Size:          9     312
 
EcoO109I rG'GnC_Cy

Cuts at:      0     188     321 
   Size:        188     133
 
EcoRI G'AATT_C

Cuts at:      0     152     321 
   Size:        152     169
 
HaeII r_GCGC'y

Cuts at:      0      58     321 
   Size:         58     263
 
HindIII A'AGCT_T

Cuts at:      0     248     321 
   Size:        248      73
 
NaeI GCC'GGC

Cuts at:      0      88     321 
   Size:         88     233
 
NcoI C'CATG_G

Cuts at:      0     272     321 
   Size:        272      49
 
NgoMIV G'CCGG_C

Cuts at:      0      86     321 
   Size:         86     235
 
PpuMI rG'GwC_Cy

Cuts at:      0     188     321 
   Size:        188     133
 
PstI C_TGCA'G

Cuts at:      0     149     321 
   Size:        149     172
 
SfcI C'TryA_G

Cuts at:      0     145     321 
   Size:        145     176
 
SmlI C'TyrA_G

Cuts at:      0     160     232     321 
   Size:        160      72      89

  Fragments arranged by size: 

                 160      89      72
 
StyI C'CwwG_G

Cuts at:      0     272     321 
   Size:        272      49
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      92     124     321 
   Size:         92      32     197

  Fragments arranged by size: 

                 197      92      32
 

 Enzymes that do cut:

    AlwNI     ApoI    BpuEI    BsaXI    BseYI  BsiHKAI Bsp1286I    BsrFI
   BssHII   BstAPI     BtgI     BtsI     EaeI     EciI EcoO109I    EcoRI
    HaeII  HindIII     NaeI     NcoI   NgoMIV    PpuMI     PstI     SfcI
     SmlI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI