Restriction map for
pFN21AE1320
(Linear) (Six-base) MAPSORT of: KIEE1320.seq Check: 5486 from: 1 to: 1293
KIEE1320.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 289 1009 1293
Size: 200 89 720 284
Fragments arranged by size:
720 284 200 89
AhdI GACnn_n'nnGTC
Cuts at: 0 606 1293
Size: 606 687
AleI CACnn'nnGTG
Cuts at: 0 535 1228 1293
Size: 535 693 65
Fragments arranged by size:
693 535 65
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1293
Size: 1144 32 117
Fragments arranged by size:
1144 117 32
AvaI C'yCGr_G
Cuts at: 0 562 906 948 1009 1293
Size: 562 344 42 61 284
Fragments arranged by size:
562 344 284 61 42
BamHI G'GATC_C
Cuts at: 0 259 1293
Size: 259 1034
BanI G'GyrC_C
Cuts at: 0 541 558 1293
Size: 541 17 735
Fragments arranged by size:
735 541 17
BanII G_rGCy'C
Cuts at: 0 100 860 913 1023 1293
Size: 100 760 53 110 270
Fragments arranged by size:
760 270 110 100 53
BciVI GTATCCnnnnn_n'
Cuts at: 0 267 1293
Size: 267 1026
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1293
Size: 32 1261
BglII A'GATC_T
Cuts at: 0 324 1293
Size: 324 969
Bme1580I G_kGCm'C
Cuts at: 0 544 1210 1293
Size: 544 666 83
Fragments arranged by size:
666 544 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1293
Size: 1280 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1293
Size: 1237 56
Bpu10I CC'TnA_GC
Cuts at: 0 794 1293
Size: 794 499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1293
Size: 724 569
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1189 1219 1293
Size: 895 30 264 30 74
Fragments arranged by size:
895 264 74 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1097 1213 1293
Size: 1097 116 80
Fragments arranged by size:
1097 116 80
BseYI C'CCAG_C
Cuts at: 0 1235 1293
Size: 1235 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 649 1264 1293
Size: 37 612 615 29
Fragments arranged by size:
615 612 37 29
BsiHKAI G_wGCw'C
Cuts at: 0 860 913 1023 1293
Size: 860 53 110 270
Fragments arranged by size:
860 270 110 53
Bsp1286I G_dGCh'C
Cuts at: 0 100 544 860 913 1023 1210 1293
Size: 100 444 316 53 110 187 83
Fragments arranged by size:
444 316 187 110 100 83 53
BsrDI GCAATG_nn'
Cuts at: 0 834 1293
Size: 834 459
BssSI C'ACGA_G
Cuts at: 0 1240 1293
Size: 1240 53
BstYI r'GATC_y
Cuts at: 0 259 324 974 996 1293
Size: 259 65 650 22 297
Fragments arranged by size:
650 297 259 65 22
BtgI C'CryG_G
Cuts at: 0 1226 1293
Size: 1226 67
BtsI GCAGTG_nn'
Cuts at: 0 788 963 1293
Size: 788 175 330
Fragments arranged by size:
788 330 175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 695 730 1293
Size: 695 35 563
Fragments arranged by size:
695 563 35
EaeI y'GGCC_r
Cuts at: 0 440 498 939 1293
Size: 440 58 441 354
Fragments arranged by size:
441 440 354 58
EarI CTCTTCn'nnn_
Cuts at: 0 56 1028 1126 1163 1293
Size: 56 972 98 37 130
Fragments arranged by size:
972 130 98 56 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 1293
Size: 496 797
EcoICRI GAG'CTC
Cuts at: 0 858 911 1021 1293
Size: 858 53 110 272
Fragments arranged by size:
858 272 110 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 289 1009 1237 1293
Size: 200 89 720 228 56
Fragments arranged by size:
720 228 200 89 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 1293
Size: 1195 98
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 1293
Size: 117 1176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 592 624 895 927 1169 1201
Size: 150 32 410 32 271 32 242 32
Cuts at: 1201 1293
Size: 92
Fragments arranged by size:
410 271 242 150 92 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 947 1244 1293
Size: 695 252 297 49
Fragments arranged by size:
695 297 252 49
MscI TGG'CCA
Cuts at: 0 500 941 1293
Size: 500 441 352
Fragments arranged by size:
500 441 352
MslI CAynn'nnrTG
Cuts at: 0 535 1228 1293
Size: 535 693 65
Fragments arranged by size:
693 535 65
MspA1I CmG'CkG
Cuts at: 0 504 1248 1293
Size: 504 744 45
Fragments arranged by size:
744 504 45
NcoI C'CATG_G
Cuts at: 0 1226 1293
Size: 1226 67
NdeI CA'TA_TG
Cuts at: 0 240 1293
Size: 240 1053
NspI r_CATG'y
Cuts at: 0 39 1293
Size: 39 1254
PasI CC'CwG_GG
Cuts at: 0 612 1293
Size: 612 681
PspXI vC'TCGA_Gb
Cuts at: 0 906 1293
Size: 906 387
PvuII CAG'CTG
Cuts at: 0 504 1293
Size: 504 789
SacI G_AGCT'C
Cuts at: 0 860 913 1023 1293
Size: 860 53 110 270
Fragments arranged by size:
860 270 110 53
SapI GCTCTTCn'nnn_
Cuts at: 0 56 1028 1126 1163 1293
Size: 56 972 98 37 130
Fragments arranged by size:
972 130 98 56 37
SfcI C'TryA_G
Cuts at: 0 726 1293
Size: 726 567
SmlI C'TyrA_G
Cuts at: 0 739 906 1009 1293
Size: 739 167 103 284
Fragments arranged by size:
739 284 167 103
SphI G_CATG'C
Cuts at: 0 39 1293
Size: 39 1254
SspI AAT'ATT
Cuts at: 0 253 1293
Size: 253 1040
StuI AGG'CCT
Cuts at: 0 757 1074 1293
Size: 757 317 219
Fragments arranged by size:
757 317 219
StyI C'CwwG_G
Cuts at: 0 1226 1293
Size: 1226 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 529 868 1293
Size: 529 339 425
Fragments arranged by size:
529 425 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1293
Size: 1189 32 72
Fragments arranged by size:
1189 72 32
XhoI C'TCGA_G
Cuts at: 0 906 1009 1293
Size: 906 103 284
Fragments arranged by size:
906 284 103
XmnI GAAnn'nnTTC
Cuts at: 0 532 1293
Size: 532 761
Enzymes that do cut:
AcuI AhdI AleI AloI AvaI BamHI BanI BanII
BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BssSI
BstYI BtgI BtsI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoNI FalI Hin4I MmeI MscI MslI MspA1I
NcoI NdeI NspI PasI PspXI PvuII SacI SapI
SfcI SmlI SphI SspI StuI StyI TaqII TstI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BclI
BfrBI BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI TatI Tth111I XbaI XcmI
XmaI ZraI