Restriction map for
pFN21AE1318
(Linear) (Six-base) MAPSORT of: KIEE1318.seq Check: 2018 from: 1 to: 3606
KIEE1318.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AccI GT'mk_AC
Cuts at: 0 2966 3026 3606
Size: 2966 60 580
Fragments arranged by size:
2966 580 60
AclI AA'CG_TT
Cuts at: 0 1335 3606
Size: 1335 2271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 911 1100 1235 1879 2815 3252 3606
Size: 511 400 189 135 644 936 437 354
Fragments arranged by size:
936 644 511 437 400 354 189 135
AfeI AGC'GCT
Cuts at: 0 1108 1202 3606
Size: 1108 94 2404
Fragments arranged by size:
2404 1108 94
AflIII A'CryG_T
Cuts at: 0 1796 3606
Size: 1796 1810
AhdI GACnn_n'nnGTC
Cuts at: 0 2149 3606
Size: 2149 1457
AleI CACnn'nnGTG
Cuts at: 0 1012 3606
Size: 1012 2594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2800 3606
Size: 2800 806
AlwNI CAG_nnn'CTG
Cuts at: 0 1143 1348 3606
Size: 1143 205 2258
Fragments arranged by size:
2258 1143 205
ApaLI G'TGCA_C
Cuts at: 0 3272 3606
Size: 3272 334
ApoI r'AATT_y
Cuts at: 0 3337 3606
Size: 3337 269
AvaI C'yCGr_G
Cuts at: 0 2129 2235 3606
Size: 2129 106 1371
Fragments arranged by size:
2129 1371 106
BamHI G'GATC_C
Cuts at: 0 2111 3176 3606
Size: 2111 1065 430
Fragments arranged by size:
2111 1065 430
BanI G'GyrC_C
Cuts at: 0 589 3606
Size: 589 3017
BanII G_rGCy'C
Cuts at: 0 764 2038 2244 2666 3606
Size: 764 1274 206 422 940
Fragments arranged by size:
1274 940 764 422 206
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 3503 3606
Size: 746 2757 103
Fragments arranged by size:
2757 746 103
BbvCI CC'TCA_GC
Cuts at: 0 1831 2800 3606
Size: 1831 969 806
Fragments arranged by size:
1831 969 806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1771 1805 3253 3287 3606
Size: 1771 34 1448 34 319
Fragments arranged by size:
1771 1448 319 34 34
BfrBI ATG'CAT
Cuts at: 0 1068 3606
Size: 1068 2538
BglI GCCn_nnn'nGGC
Cuts at: 0 1860 3606
Size: 1860 1746
BglII A'GATC_T
Cuts at: 0 709 876 3606
Size: 709 167 2730
Fragments arranged by size:
2730 709 167
BlpI GC'TnA_GC
Cuts at: 0 3453 3606
Size: 3453 153
Bme1580I G_kGCm'C
Cuts at: 0 1743 3276 3606
Size: 1743 1533 330
Fragments arranged by size:
1743 1533 330
BmgBI CAC'GTC
Cuts at: 0 1797 3141 3606
Size: 1797 1344 465
Fragments arranged by size:
1797 1344 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 2155 2928 3606
Size: 2155 773 678
Fragments arranged by size:
2155 773 678
BmtI G_CTAG'C
Cuts at: 0 2072 3606
Size: 2072 1534
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 185 3606
Size: 185 3421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 381 434 3606
Size: 68 313 53 3172
Fragments arranged by size:
3172 313 68 53
Bpu10I CC'TnA_GC
Cuts at: 0 1831 2800 3606
Size: 1831 969 806
Fragments arranged by size:
1831 969 806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 3606
Size: 155 3451
BsaI GGTCTCn'nnnn_
Cuts at: 0 126 416 3606
Size: 126 290 3190
Fragments arranged by size:
3190 290 126
BsaBI GATnn'nnATC
Cuts at: 0 1490 2770 3606
Size: 1490 1280 836
Fragments arranged by size:
1490 1280 836
BsaHI Gr'CG_yC
Cuts at: 0 1910 2150 2969 3606
Size: 1910 240 819 637
Fragments arranged by size:
1910 819 637 240
BsaWI w'CCGG_w
Cuts at: 0 3089 3606
Size: 3089 517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1615 1645 2598 2628 2809 2839 3606
Size: 1615 30 953 30 181 30 767
Fragments arranged by size:
1615 953 767 181 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 65 1285 1819 1987 2064 3606
Size: 65 1220 534 168 77 1542
Fragments arranged by size:
1542 1220 534 168 77 65
BseYI C'CCAG_C
Cuts at: 0 1742 1855 2124 2350 2592 2917 3460 3606
Size: 1742 113 269 226 242 325 543 146
Fragments arranged by size:
1742 543 325 269 242 226 146 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1667 2245 2260 2770 3606
Size: 977 690 578 15 510 836
Fragments arranged by size:
977 836 690 578 510 15
BsiHKAI G_wGCw'C
Cuts at: 0 764 2038 2666 3240 3276 3411 3606
Size: 764 1274 628 574 36 135 195
Fragments arranged by size:
1274 764 628 574 195 135 36
BsmI GAATG_Cn'
Cuts at: 0 391 1777 3606
Size: 391 1386 1829
Fragments arranged by size:
1829 1386 391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1150 2494 3606
Size: 1150 1344 1112
Fragments arranged by size:
1344 1150 1112
Bsp1286I G_dGCh'C
Cuts at: 0 764 1743 2038 2244 2666 3240 3276 3411
Size: 764 979 295 206 422 574 36 135
Cuts at: 3411 3606
Size: 195
Fragments arranged by size:
979 764 574 422 295 206 195 135
36
BspHI T'CATG_A
Cuts at: 0 216 2153 3606
Size: 216 1937 1453
Fragments arranged by size:
1937 1453 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1853 3606
Size: 1853 1753
BsrBI CCG'CTC
Cuts at: 0 408 3606
Size: 408 3198
BsrDI GCAATG_nn'
Cuts at: 0 3162 3558 3606
Size: 3162 396 48
Fragments arranged by size:
3162 396 48
BsrFI r'CCGG_y
Cuts at: 0 1673 2255 3606
Size: 1673 582 1351
Fragments arranged by size:
1673 1351 582
BssSI C'ACGA_G
Cuts at: 0 205 696 733 2290 2890 3317 3606
Size: 205 491 37 1557 600 427 289
Fragments arranged by size:
1557 600 491 427 289 205 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1131 2589 3606
Size: 1131 1458 1017
Fragments arranged by size:
1458 1131 1017
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 2979 3606
Size: 471 2508 627
Fragments arranged by size:
2508 627 471
BstYI r'GATC_y
Cuts at: 0 709 855 876 2111 2753 3176 3293 3606
Size: 709 146 21 1235 642 423 117 313
Fragments arranged by size:
1235 709 642 423 313 146 117 21
BtgI C'CryG_G
Cuts at: 0 63 425 932 1610 3606
Size: 63 362 507 678 1996
Fragments arranged by size:
1996 678 507 362 63
BtsI GCAGTG_nn'
Cuts at: 0 1593 1655 2001 2094 3280 3606
Size: 1593 62 346 93 1186 326
Fragments arranged by size:
1593 1186 346 326 93 62
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2630 2665 3606
Size: 2630 35 941
Fragments arranged by size:
2630 941 35
DraIII CAC_nnn'GTG
Cuts at: 0 1000 3606
Size: 1000 2606
DrdI GACnn_nn'nnGTC
Cuts at: 0 2838 3606
Size: 2838 768
EaeI y'GGCC_r
Cuts at: 0 1569 1620 2421 2807 3606
Size: 1569 51 801 386 799
Fragments arranged by size:
1569 801 799 386 51
EarI CTCTTCn'nnn_
Cuts at: 0 427 572 845 950 1211 2174 3606
Size: 427 145 273 105 261 963 1432
Fragments arranged by size:
1432 963 427 273 261 145 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1192 3606
Size: 1192 2414
EcoICRI GAG'CTC
Cuts at: 0 762 2036 2664 3606
Size: 762 1274 628 942
Fragments arranged by size:
1274 942 762 628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 381 434 511 911 1100 1235 1879
Size: 68 313 53 77 400 189 135 644
Cuts at: 1879 2815 3252 3606
Size: 936 437 354
Fragments arranged by size:
936 644 437 400 354 313 189 135
77 68 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 50 3606
Size: 50 3556
EcoO109I rG'GnC_Cy
Cuts at: 0 40 3606
Size: 40 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1571 3160 3606
Size: 1571 1589 446
Fragments arranged by size:
1589 1571 446
HaeII r_GCGC'y
Cuts at: 0 1110 1204 2097 2275 3606
Size: 1110 94 893 178 1331
Fragments arranged by size:
1331 1110 893 178 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1414 1446 1834 1866 1968 2000 2135 2167
Size: 1414 32 388 32 102 32 135 32
Cuts at: 2167 2497 2529 2809 2841 3144 3176 3606
Size: 330 32 280 32 303 32 430
Fragments arranged by size:
1414 430 388 330 303 280 135 102
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1353 1842 3027 3606
Size: 1353 489 1185 579
Fragments arranged by size:
1353 1185 579 489
HindIII A'AGCT_T
Cuts at: 0 3396 3606
Size: 3396 210
MfeI C'AATT_G
Cuts at: 0 3185 3606
Size: 3185 421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 2768 3554 3606
Size: 1247 1521 786 52
Fragments arranged by size:
1521 1247 786 52
MscI TGG'CCA
Cuts at: 0 1622 2809 3606
Size: 1622 1187 797
Fragments arranged by size:
1622 1187 797
MslI CAynn'nnrTG
Cuts at: 0 1012 3414 3606
Size: 1012 2402 192
Fragments arranged by size:
2402 1012 192
MspA1I CmG'CkG
Cuts at: 0 1293 1859 1905 1965 3015 3606
Size: 1293 566 46 60 1050 591
Fragments arranged by size:
1293 1050 591 566 60 46
NaeI GCC'GGC
Cuts at: 0 1675 3606
Size: 1675 1931
NcoI C'CATG_G
Cuts at: 0 63 932 1610 3606
Size: 63 869 678 1996
Fragments arranged by size:
1996 869 678 63
NgoMIV G'CCGG_C
Cuts at: 0 1673 3606
Size: 1673 1933
NheI G'CTAG_C
Cuts at: 0 2068 3606
Size: 2068 1538
NsiI A_TGCA'T
Cuts at: 0 1070 3606
Size: 1070 2536
PasI CC'CwG_GG
Cuts at: 0 192 3606
Size: 192 3414
PflMI CCAn_nnn'nTGG
Cuts at: 0 1194 3606
Size: 1194 2412
PfoI T'CCnGG_A
Cuts at: 0 1163 2813 3606
Size: 1163 1650 793
Fragments arranged by size:
1650 1163 793
PstI C_TGCA'G
Cuts at: 0 1292 3606
Size: 1292 2314
PvuII CAG'CTG
Cuts at: 0 1905 3015 3606
Size: 1905 1110 591
Fragments arranged by size:
1905 1110 591
SacI G_AGCT'C
Cuts at: 0 764 2038 2666 3606
Size: 764 1274 628 940
Fragments arranged by size:
1274 940 764 628
SalI G'TCGA_C
Cuts at: 0 3025 3606
Size: 3025 581
SfcI C'TryA_G
Cuts at: 0 1288 1635 3584 3606
Size: 1288 347 1949 22
Fragments arranged by size:
1949 1288 347 22
SmlI C'TyrA_G
Cuts at: 0 170 3606
Size: 170 3436
StyI C'CwwG_G
Cuts at: 0 63 932 1208 1610 1703 2525 3606
Size: 63 869 276 402 93 822 1081
Fragments arranged by size:
1081 869 822 402 276 93 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 240 272 1615 1647 2096 2128 3606
Size: 240 32 1343 32 449 32 1478
Fragments arranged by size:
1478 1343 449 240 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2004 2857 3606
Size: 2004 853 749
Fragments arranged by size:
2004 853 749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2764 3606
Size: 2764 842
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BtgI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NsiI PasI PflMI
PfoI PstI PvuII SacI SalI SfcI SmlI StyI
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AloI ApaI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BclI BsaAI
BsiEI BsiWI BspEI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI EagI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI NarI NdeI
NotI NruI NspI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XhoI XmaI XmnI
ZraI