Restriction map for
pFN21AE1317
(Linear) (Six-base) MAPSORT of: KIEE1317.seq Check: 7118 from: 1 to: 2214
KIEE1317.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AatII G_ACGT'C
Cuts at: 0 1415 2214
Size: 1415 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1427 2214
Size: 311 1116 787
Fragments arranged by size:
1116 787 311
AflIII A'CryG_T
Cuts at: 0 905 2214
Size: 905 1309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1455 2214
Size: 1455 759
AlwNI CAG_nnn'CTG
Cuts at: 0 219 2214
Size: 219 1995
ApaI G_GGCC'C
Cuts at: 0 18 2214
Size: 18 2196
ApaLI G'TGCA_C
Cuts at: 0 765 2214
Size: 765 1449
ApoI r'AATT_y
Cuts at: 0 543 558 574 2214
Size: 543 15 16 1640
Fragments arranged by size:
1640 543 16 15
AvaI C'yCGr_G
Cuts at: 0 548 2214
Size: 548 1666
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1079 1112 2214
Size: 1079 33 1102
Fragments arranged by size:
1102 1079 33
BamHI G'GATC_C
Cuts at: 0 638 2214
Size: 638 1576
BanII G_rGCy'C
Cuts at: 0 18 750 2214
Size: 18 732 1464
Fragments arranged by size:
1464 732 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1415 2214
Size: 1415 799
BbvCI CC'TCA_GC
Cuts at: 0 206 2088 2214
Size: 206 1882 126
Fragments arranged by size:
1882 206 126
BclI T'GATC_A
Cuts at: 0 734 1832 1847 2214
Size: 734 1098 15 367
Fragments arranged by size:
1098 734 367 15
BglI GCCn_nnn'nGGC
Cuts at: 0 22 1660 2214
Size: 22 1638 554
Fragments arranged by size:
1638 554 22
BglII A'GATC_T
Cuts at: 0 2106 2214
Size: 2106 108
BlpI GC'TnA_GC
Cuts at: 0 749 2214
Size: 749 1465
Bme1580I G_kGCm'C
Cuts at: 0 18 392 769 2214
Size: 18 374 377 1445
Fragments arranged by size:
1445 377 374 18
BmgBI CAC'GTC
Cuts at: 0 198 237 2061 2214
Size: 198 39 1824 153
Fragments arranged by size:
1824 198 153 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 386 1245 2214
Size: 386 859 969
Fragments arranged by size:
969 859 386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 769 2214
Size: 769 1445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 1565 1728 2214
Size: 1043 522 163 486
Fragments arranged by size:
1043 522 486 163
Bpu10I CC'TnA_GC
Cuts at: 0 206 2088 2214
Size: 206 1882 126
Fragments arranged by size:
1882 206 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1091 2214
Size: 94 997 1123
Fragments arranged by size:
1123 997 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 545 2214
Size: 545 1669
BsaAI yAC'GTr
Cuts at: 0 908 2214
Size: 908 1306
BsaBI GATnn'nnATC
Cuts at: 0 1516 2214
Size: 1516 698
BsaHI Gr'CG_yC
Cuts at: 0 1412 2214
Size: 1412 802
BsaWI w'CCGG_w
Cuts at: 0 296 727 2147 2214
Size: 296 431 1420 67
Fragments arranged by size:
1420 431 296 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 241 268 271 1690 1720 1726 1756
Size: 238 3 27 3 1419 30 6 30
Cuts at: 1756 2214
Size: 458
Fragments arranged by size:
1419 458 238 30 30 27 6 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 2214
Size: 246 1968
BseYI C'CCAG_C
Cuts at: 0 1226 2214
Size: 1226 988
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1698 2083 2214
Size: 1140 558 385 131
Fragments arranged by size:
1140 558 385 131
BsiHKAI G_wGCw'C
Cuts at: 0 271 750 769 893 1056 2214
Size: 271 479 19 124 163 1158
Fragments arranged by size:
1158 479 271 163 124 19
Bsp1286I G_dGCh'C
Cuts at: 0 18 271 392 750 769 893 1056 2214
Size: 18 253 121 358 19 124 163 1158
Fragments arranged by size:
1158 358 253 163 124 121 19 18
BspEI T'CCGG_A
Cuts at: 0 296 2147 2214
Size: 296 1851 67
Fragments arranged by size:
1851 296 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1153 2214
Size: 1153 1061
BsrDI GCAATG_nn'
Cuts at: 0 1570 1835 1850 2024 2214
Size: 1570 265 15 174 190
Fragments arranged by size:
1570 265 190 174 15
BsrGI T'GTAC_A
Cuts at: 0 2135 2214
Size: 2135 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 2214
Size: 216 1998
BstEII G'GTnAC_C
Cuts at: 0 678 2214
Size: 678 1536
BstYI r'GATC_y
Cuts at: 0 299 638 1582 1959 2106 2214
Size: 299 339 944 377 147 108
Fragments arranged by size:
944 377 339 299 147 108
BtgI C'CryG_G
Cuts at: 0 881 1792 2168 2214
Size: 881 911 376 46
Fragments arranged by size:
911 881 376 46
BtsI GCAGTG_nn'
Cuts at: 0 172 356 1157 1820 2214
Size: 172 184 801 663 394
Fragments arranged by size:
801 663 394 184 172
ClaI AT'CG_AT
Cuts at: 0 1297 2214
Size: 1297 917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1514 1549 2214
Size: 1514 35 665
Fragments arranged by size:
1514 665 35
DraI TTT'AAA
Cuts at: 0 1334 2214
Size: 1334 880
DraIII CAC_nnn'GTG
Cuts at: 0 32 2214
Size: 32 2182
DrdI GACnn_nn'nnGTC
Cuts at: 0 1177 2214
Size: 1177 1037
EaeI y'GGCC_r
Cuts at: 0 878 1856 2214
Size: 878 978 358
Fragments arranged by size:
978 878 358
EarI CTCTTCn'nnn_
Cuts at: 0 112 259 2214
Size: 112 147 1955
Fragments arranged by size:
1955 147 112
EcoICRI GAG'CTC
Cuts at: 0 748 2214
Size: 748 1466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1043 1427 1565 1728 2214
Size: 311 732 384 138 163 486
Fragments arranged by size:
732 486 384 311 163 138
EcoNI CCTnn'n_nnAGG
Cuts at: 0 790 2214
Size: 790 1424
EcoO109I rG'GnC_Cy
Cuts at: 0 474 2214
Size: 474 1740
EcoRI G'AATT_C
Cuts at: 0 543 574 2214
Size: 543 31 1640
Fragments arranged by size:
1640 543 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1262 1294 1824 1856 1939 1971 2214
Size: 1262 32 530 32 83 32 243
Fragments arranged by size:
1262 530 243 83 32 32 32
HincII GTy'rAC
Cuts at: 0 111 450 957 2214
Size: 111 339 507 1257
Fragments arranged by size:
1257 507 339 111
MfeI C'AATT_G
Cuts at: 0 491 2214
Size: 491 1723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1964 1986 2214
Size: 1115 849 22 228
Fragments arranged by size:
1115 849 228 22
MscI TGG'CCA
Cuts at: 0 880 2214
Size: 880 1334
MslI CAynn'nnrTG
Cuts at: 0 321 730 775 880 1828 2214
Size: 321 409 45 105 948 386
Fragments arranged by size:
948 409 386 321 105 45
MspA1I CmG'CkG
Cuts at: 0 2092 2214
Size: 2092 122
NcoI C'CATG_G
Cuts at: 0 881 1792 2214
Size: 881 911 422
Fragments arranged by size:
911 881 422
NdeI CA'TA_TG
Cuts at: 0 644 2214
Size: 644 1570
PasI CC'CwG_GG
Cuts at: 0 398 2214
Size: 398 1816
PflMI CCAn_nnn'nTGG
Cuts at: 0 32 375 2214
Size: 32 343 1839
Fragments arranged by size:
1839 343 32
PmlI CAC'GTG
Cuts at: 0 908 2214
Size: 908 1306
PspOMI G'GGCC_C
Cuts at: 0 14 2214
Size: 14 2200
PstI C_TGCA'G
Cuts at: 0 167 363 1164 2214
Size: 167 196 801 1050
Fragments arranged by size:
1050 801 196 167
PvuII CAG'CTG
Cuts at: 0 2092 2214
Size: 2092 122
SacI G_AGCT'C
Cuts at: 0 750 2214
Size: 750 1464
SapI GCTCTTCn'nnn_
Cuts at: 0 112 259 2214
Size: 112 147 1955
Fragments arranged by size:
1955 147 112
SexAI A'CCwGG_T
Cuts at: 0 368 2214
Size: 368 1846
SfcI C'TryA_G
Cuts at: 0 163 359 1160 2214
Size: 163 196 801 1054
Fragments arranged by size:
1054 801 196 163
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 22 2214
Size: 22 2192
SmlI C'TyrA_G
Cuts at: 0 73 548 1106 2214
Size: 73 475 558 1108
Fragments arranged by size:
1108 558 475 73
StyI C'CwwG_G
Cuts at: 0 18 429 881 935 1014 1792 1883 2214
Size: 18 411 452 54 79 778 91 331
Fragments arranged by size:
778 452 411 331 91 79 54 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 720 1672 2214
Size: 720 952 542
Fragments arranged by size:
952 720 542
TatI w'GTAC_w
Cuts at: 0 325 866 2066 2135 2214
Size: 325 541 1200 69 79
Fragments arranged by size:
1200 541 325 79 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 2214
Size: 695 32 1487
Fragments arranged by size:
1487 695 32
Tth111I GACn'n_nGTC
Cuts at: 0 2002 2214
Size: 2002 212
XbaI T'CTAG_A
Cuts at: 0 2185 2214
Size: 2185 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 405 672 847 984 2214
Size: 405 267 175 137 1230
Fragments arranged by size:
1230 405 267 175 137
XhoI C'TCGA_G
Cuts at: 0 548 2214
Size: 548 1666
ZraI GAC'GTC
Cuts at: 0 1413 2214
Size: 1413 801
Enzymes that do cut:
AatII AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanII BbsI BbvCI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrGI
BstAPI BstEII BstYI BtgI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI Hin4I HincII MfeI MmeI MscI MslI MspA1I
NcoI NdeI PasI PflMI PmlI PspOMI PstI PvuII
SacI SapI SexAI SfcI SfiI SmlI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BanI BbeI
BcgI BciVI BfrBI BmtI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrFI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI EagI EciI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI XmaI XmnI