(Linear) (Six-base) MAPSORT of: KIEE1316.seq  Check: 982  from: 1  to: 819



KIEE1316.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AflIII A'CryG_T

Cuts at:      0     764     819 
   Size:        764      55
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     254     286     819 
   Size:        254      32     533

  Fragments arranged by size: 

                 533     254      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     286     819 
   Size:        286     533
 
ApoI r'AATT_y

Cuts at:      0     295     331     487     652     819 
   Size:        295      36     156     165     167

  Fragments arranged by size: 

                 295     167     165     156      36
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16     127     819 
   Size:         16     111     692

  Fragments arranged by size: 

                 692     111      16
 
Bme1580I G_kGCm'C

Cuts at:      0     105     819 
   Size:        105     714
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     819 
   Size:        188     631
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     572     819 
   Size:        572     247
 
BsaBI GATnn'nnATC

Cuts at:      0     344     819 
   Size:        344     475
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     230     819 
   Size:        230     589
 
BsiEI CG_ry'CG

Cuts at:      0      11     819 
   Size:         11     808
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     706     819 
   Size:        706     113
 
Bsp1286I G_dGCh'C

Cuts at:      0     105     819 
   Size:        105     714
 
BsrBI CCG'CTC

Cuts at:      0      87     819 
   Size:         87     732
 
BtgI C'CryG_G

Cuts at:      0      50     819 
   Size:         50     769
 
EaeI y'GGCC_r

Cuts at:      0       8     792     819 
   Size:          8     784      27

  Fragments arranged by size: 

                 784      27       8
 
EagI C'GGCC_G

Cuts at:      0       8     819 
   Size:          8     811
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     556     819 
   Size:        227     329     263

  Fragments arranged by size: 

                 329     263     227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     819 
   Size:        188     631
 
EcoO109I rG'GnC_Cy

Cuts at:      0     283     819 
   Size:        283     536
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     460     819 
   Size:        460     359
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     708     740     819 
   Size:        708      32      79

  Fragments arranged by size: 

                 708      79      32
 
HindIII A'AGCT_T

Cuts at:      0     409     819 
   Size:        409     410
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     642     819 
   Size:        642     177
 
MscI TGG'CCA

Cuts at:      0     794     819 
   Size:        794      25
 
MslI CAynn'nnrTG

Cuts at:      0     728     734     819 
   Size:        728       6      85

  Fragments arranged by size: 

                 728      85       6
 
MspA1I CmG'CkG

Cuts at:      0      52      99     819 
   Size:         52      47     720

  Fragments arranged by size: 

                 720      52      47
 
NdeI CA'TA_TG

Cuts at:      0     807     819 
   Size:        807      12
 
NspI r_CATG'y

Cuts at:      0     768     819 
   Size:        768      51
 
PciI A'CATG_T

Cuts at:      0     764     819 
   Size:        764      55
 
PpuMI rG'GwC_Cy

Cuts at:      0     283     819 
   Size:        283     536
 
PsiI TTA'TAA

Cuts at:      0     415     819 
   Size:        415     404
 
PstI C_TGCA'G

Cuts at:      0     388     819 
   Size:        388     431
 
SacII CC_GC'GG

Cuts at:      0      53     819 
   Size:         53     766
 
SfcI C'TryA_G

Cuts at:      0     384     819 
   Size:        384     435
 
TatI w'GTAC_w

Cuts at:      0     781     819 
   Size:        781      38
 
XmnI GAAnn'nnTTC

Cuts at:      0     443     819 
   Size:        443     376
 

 Enzymes that do cut:

   AflIII     AloI    AlwNI     ApoI     BglI Bme1580I     BpmI     BsaI
    BsaBI    BseRI    BsiEI    BsmBI Bsp1286I    BsrBI     BtgI     EaeI
     EagI     EarI  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NdeI     NspI     PciI    PpuMI     PsiI
     PstI    SacII     SfcI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI