Restriction map for
pFN21AE1315
(Linear) (Six-base) MAPSORT of: KIEE1315.seq Check: 7484 from: 1 to: 927
KIEE1315.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AccI GT'mk_AC
Cuts at: 0 704 927
Size: 704 223
AfeI AGC'GCT
Cuts at: 0 756 927
Size: 756 171
AleI CACnn'nnGTG
Cuts at: 0 22 412 927
Size: 22 390 515
Fragments arranged by size:
515 390 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 927
Size: 693 234
ApaI G_GGCC'C
Cuts at: 0 223 797 927
Size: 223 574 130
Fragments arranged by size:
574 223 130
AvaI C'yCGr_G
Cuts at: 0 443 500 797 911 927
Size: 443 57 297 114 16
Fragments arranged by size:
443 297 114 57 16
BanI G'GyrC_C
Cuts at: 0 190 379 927
Size: 190 189 548
Fragments arranged by size:
548 190 189
BanII G_rGCy'C
Cuts at: 0 223 331 797 891 927
Size: 223 108 466 94 36
Fragments arranged by size:
466 223 108 94 36
BciVI GTATCCnnnnn_n'
Cuts at: 0 96 927
Size: 96 831
BglI GCCn_nnn'nGGC
Cuts at: 0 667 895 927
Size: 667 228 32
Fragments arranged by size:
667 228 32
BglII A'GATC_T
Cuts at: 0 81 927
Size: 81 846
BlpI GC'TnA_GC
Cuts at: 0 575 927
Size: 575 352
Bme1580I G_kGCm'C
Cuts at: 0 29 193 223 384 771 797 927
Size: 29 164 30 161 387 26 130
Fragments arranged by size:
387 164 161 130 30 29 26
BmgBI CAC'GTC
Cuts at: 0 708 927
Size: 708 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 927
Size: 165 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 766 927
Size: 470 296 161
Fragments arranged by size:
470 296 161
Bpu10I CC'TnA_GC
Cuts at: 0 437 927
Size: 437 490
BsaWI w'CCGG_w
Cuts at: 0 116 927
Size: 116 811
BseYI C'CCAG_C
Cuts at: 0 401 927
Size: 401 526
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 888 927
Size: 663 225 39
Fragments arranged by size:
663 225 39
Bsp1286I G_dGCh'C
Cuts at: 0 29 193 223 331 384 771 797 891
Size: 29 164 30 108 53 387 26 94
Cuts at: 891 927
Size: 36
Fragments arranged by size:
387 164 108 94 53 36 30 29
26
BspHI T'CATG_A
Cuts at: 0 626 927
Size: 626 301
BsrDI GCAATG_nn'
Cuts at: 0 494 927
Size: 494 433
BsrFI r'CCGG_y
Cuts at: 0 250 927
Size: 250 677
BssHII G'CGCG_C
Cuts at: 0 153 927
Size: 153 774
BstEII G'GTnAC_C
Cuts at: 0 582 927
Size: 582 345
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 927
Size: 414 513
BstYI r'GATC_y
Cuts at: 0 81 125 927
Size: 81 44 802
Fragments arranged by size:
802 81 44
BtgI C'CryG_G
Cuts at: 0 177 203 407 927
Size: 177 26 204 520
Fragments arranged by size:
520 204 177 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 60 675 927
Size: 60 615 252
Fragments arranged by size:
615 252 60
EaeI y'GGCC_r
Cuts at: 0 759 927
Size: 759 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 766 927
Size: 470 296 161
Fragments arranged by size:
470 296 161
EcoNI CCTnn'n_nnAGG
Cuts at: 0 598 927
Size: 598 329
EcoO109I rG'GnC_Cy
Cuts at: 0 219 220 519 533 793 794 927
Size: 219 1 299 14 260 1 133
Fragments arranged by size:
299 260 219 133 14 1 1
HaeII r_GCGC'y
Cuts at: 0 170 264 758 927
Size: 170 94 494 169
Fragments arranged by size:
494 170 169 94
HincII GTy'rAC
Cuts at: 0 567 927
Size: 567 360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 927
Size: 439 488
MscI TGG'CCA
Cuts at: 0 761 927
Size: 761 166
MslI CAynn'nnrTG
Cuts at: 0 22 412 927
Size: 22 390 515
Fragments arranged by size:
515 390 22
MspA1I CmG'CkG
Cuts at: 0 67 179 879 927
Size: 67 112 700 48
Fragments arranged by size:
700 112 67 48
NaeI GCC'GGC
Cuts at: 0 252 927
Size: 252 675
NcoI C'CATG_G
Cuts at: 0 203 927
Size: 203 724
NgoMIV G'CCGG_C
Cuts at: 0 250 927
Size: 250 677
NspI r_CATG'y
Cuts at: 0 340 927
Size: 340 587
PasI CC'CwG_GG
Cuts at: 0 738 927
Size: 738 189
PfoI T'CCnGG_A
Cuts at: 0 844 927
Size: 844 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 927
Size: 496 32 399
Fragments arranged by size:
496 399 32
PpuMI rG'GwC_Cy
Cuts at: 0 533 927
Size: 533 394
PspOMI G'GGCC_C
Cuts at: 0 219 793 927
Size: 219 574 134
Fragments arranged by size:
574 219 134
PstI C_TGCA'G
Cuts at: 0 260 927
Size: 260 667
PvuII CAG'CTG
Cuts at: 0 67 879 927
Size: 67 812 48
Fragments arranged by size:
812 67 48
SacII CC_GC'GG
Cuts at: 0 180 927
Size: 180 747
SfcI C'TryA_G
Cuts at: 0 158 256 701 927
Size: 158 98 445 226
Fragments arranged by size:
445 226 158 98
SmaI CCC'GGG
Cuts at: 0 445 927
Size: 445 482
SphI G_CATG'C
Cuts at: 0 340 927
Size: 340 587
SrfI GCCC'GGGC
Cuts at: 0 445 927
Size: 445 482
StyI C'CwwG_G
Cuts at: 0 129 203 323 927
Size: 129 74 120 604
Fragments arranged by size:
604 129 120 74
TatI w'GTAC_w
Cuts at: 0 746 927
Size: 746 181
Tth111I GACn'n_nGTC
Cuts at: 0 709 927
Size: 709 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 381 927
Size: 201 180 546
Fragments arranged by size:
546 201 180
XmaI C'CCGG_G
Cuts at: 0 443 927
Size: 443 484
Enzymes that do cut:
AccI AfeI AleI AlfI ApaI AvaI BanI BanII
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaWI BseYI BsgI Bsp1286I BspHI BsrDI BsrFI
BssHII BstEII BstXI BstYI BtgI BtgZI EaeI Eco57MI
EcoNI EcoO109I HaeII HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI
PspOMI PstI PvuII SacII SfcI SmaI SphI SrfI
StyI TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SspI StuI
SwaI TaqII TstI XbaI XhoI XmnI ZraI