Restriction map for
pFN21AE1314
(Linear) (Six-base) MAPSORT of: KIEE1314.seq Check: 1049 from: 1 to: 1677
KIEE1314.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 1677
Size: 1661 16
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 1677
Size: 358 1319
ApoI r'AATT_y
Cuts at: 0 630 699 724 899 1195 1510 1597 1634
Size: 630 69 25 175 296 315 87 37
Cuts at: 1634 1677
Size: 43
Fragments arranged by size:
630 315 296 175 87 69 43 37
25
BamHI G'GATC_C
Cuts at: 0 495 1677
Size: 495 1182
BanII G_rGCy'C
Cuts at: 0 139 278 413 1677
Size: 139 139 135 1264
Fragments arranged by size:
1264 139 139 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 588 697 1502 1677
Size: 588 109 805 175
Fragments arranged by size:
805 588 175 109
BbvCI CC'TCA_GC
Cuts at: 0 290 1677
Size: 290 1387
BfrBI ATG'CAT
Cuts at: 0 335 1309 1677
Size: 335 974 368
Fragments arranged by size:
974 368 335
Bme1580I G_kGCm'C
Cuts at: 0 269 1677
Size: 269 1408
BmrI ACTGGGnnnn_n'
Cuts at: 0 1380 1677
Size: 1380 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1677
Size: 162 1515
Bpu10I CC'TnA_GC
Cuts at: 0 290 502 1677
Size: 290 212 1175
Fragments arranged by size:
1175 290 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 1055 1677
Size: 1055 622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1538 1568 1677
Size: 1538 30 109
Fragments arranged by size:
1538 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 768 1677
Size: 768 909
BsiHKAI G_wGCw'C
Cuts at: 0 944 1677
Size: 944 733
Bsp1286I G_dGCh'C
Cuts at: 0 139 269 278 413 944 1677
Size: 139 130 9 135 531 733
Fragments arranged by size:
733 531 139 135 130 9
BstYI r'GATC_y
Cuts at: 0 495 1677
Size: 495 1182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 947 1677
Size: 947 730
BtsI GCAGTG_nn'
Cuts at: 0 108 1677
Size: 108 1569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 938 973 1677
Size: 938 35 704
Fragments arranged by size:
938 704 35
DraI TTT'AAA
Cuts at: 0 672 1677
Size: 672 1005
EarI CTCTTCn'nnn_
Cuts at: 0 437 1383 1677
Size: 437 946 294
Fragments arranged by size:
946 437 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1661 1677
Size: 162 1499 16
Fragments arranged by size:
1499 162 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 527 1345 1677
Size: 527 818 332
Fragments arranged by size:
818 527 332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 1677
Size: 679 32 966
Fragments arranged by size:
966 679 32
HincII GTy'rAC
Cuts at: 0 1368 1677
Size: 1368 309
HindIII A'AGCT_T
Cuts at: 0 971 1091 1327 1677
Size: 971 120 236 350
Fragments arranged by size:
971 350 236 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1677
Size: 1167 510
MslI CAynn'nnrTG
Cuts at: 0 340 1154 1677
Size: 340 814 523
Fragments arranged by size:
814 523 340
NruI TCG'CGA
Cuts at: 0 932 1677
Size: 932 745
NsiI A_TGCA'T
Cuts at: 0 337 1311 1677
Size: 337 974 366
Fragments arranged by size:
974 366 337
NspI r_CATG'y
Cuts at: 0 889 1538 1677
Size: 889 649 139
Fragments arranged by size:
889 649 139
PstI C_TGCA'G
Cuts at: 0 130 427 1677
Size: 130 297 1250
Fragments arranged by size:
1250 297 130
SfcI C'TryA_G
Cuts at: 0 126 423 1035 1677
Size: 126 297 612 642
Fragments arranged by size:
642 612 297 126
SspI AAT'ATT
Cuts at: 0 1003 1677
Size: 1003 674
StyI C'CwwG_G
Cuts at: 0 586 1140 1677
Size: 586 554 537
Fragments arranged by size:
586 554 537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 747 1677
Size: 747 930
TatI w'GTAC_w
Cuts at: 0 65 322 1677
Size: 65 257 1355
Fragments arranged by size:
1355 257 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1538 1558 1570 1590 1677
Size: 1538 20 12 20 87
Fragments arranged by size:
1538 87 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 0 1363 1677
Size: 1363 314
XmnI GAAnn'nnTTC
Cuts at: 0 631 1328 1677
Size: 631 697 349
Fragments arranged by size:
697 631 349
Enzymes that do cut:
AcuI AlfI ApoI BamHI BanII BbsI BbvCI BfrBI
Bme1580I BmrI BpmI Bpu10I BsaI BsaXI BseRI BsiHKAI
Bsp1286I BstYI BtgZI BtsI CspCI DraI EarI Eco57MI
FalI Hin4I HincII HindIII MmeI MslI NruI NsiI
NspI PstI SfcI SspI StyI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XcmI XhoI
XmaI ZraI