Restriction map for
pFN21AE1311
(Linear) (Six-base) MAPSORT of: KIEE1311.seq Check: 290 from: 1 to: 1407
KIEE1311.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AccI GT'mk_AC
Cuts at: 0 1193 1407
Size: 1193 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1407
Size: 1055 352
AflII C'TTAA_G
Cuts at: 0 54 1051 1407
Size: 54 997 356
Fragments arranged by size:
997 356 54
AlwNI CAG_nnn'CTG
Cuts at: 0 24 46 1407
Size: 24 22 1361
Fragments arranged by size:
1361 24 22
ApoI r'AATT_y
Cuts at: 0 815 1220 1232 1407
Size: 815 405 12 175
Fragments arranged by size:
815 405 175 12
AseI AT'TA_AT
Cuts at: 0 164 1244 1407
Size: 164 1080 163
Fragments arranged by size:
1080 164 163
AvaI C'yCGr_G
Cuts at: 0 203 1407
Size: 203 1204
BanII G_rGCy'C
Cuts at: 0 949 1407
Size: 949 458
BbsI GAAGACnn'nnnn_
Cuts at: 0 502 1169 1407
Size: 502 667 238
Fragments arranged by size:
667 502 238
BciVI GTATCCnnnnn_n'
Cuts at: 0 624 750 1407
Size: 624 126 657
Fragments arranged by size:
657 624 126
BglII A'GATC_T
Cuts at: 0 313 735 1332 1407
Size: 313 422 597 75
Fragments arranged by size:
597 422 313 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1043 1145 1297 1407
Size: 971 72 102 152 110
Fragments arranged by size:
971 152 110 102 72
Bpu10I CC'TnA_GC
Cuts at: 0 618 1407
Size: 618 789
BsaBI GATnn'nnATC
Cuts at: 0 312 572 1407
Size: 312 260 835
Fragments arranged by size:
835 312 260
BsaHI Gr'CG_yC
Cuts at: 0 248 1407
Size: 248 1159
BsaWI w'CCGG_w
Cuts at: 0 73 1236 1407
Size: 73 1163 171
Fragments arranged by size:
1163 171 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 587 617 1407
Size: 587 30 790
Fragments arranged by size:
790 587 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 1407
Size: 415 992
BseYI C'CCAG_C
Cuts at: 0 91 1407
Size: 91 1316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 886 1407
Size: 371 515 521
Fragments arranged by size:
521 515 371
BsiHKAI G_wGCw'C
Cuts at: 0 697 771 1407
Size: 697 74 636
Fragments arranged by size:
697 636 74
Bsp1286I G_dGCh'C
Cuts at: 0 697 771 949 1407
Size: 697 74 178 458
Fragments arranged by size:
697 458 178 74
BspEI T'CCGG_A
Cuts at: 0 1236 1407
Size: 1236 171
BsrBI CCG'CTC
Cuts at: 0 1281 1407
Size: 1281 126
BsrDI GCAATG_nn'
Cuts at: 0 1044 1407
Size: 1044 363
BstYI r'GATC_y
Cuts at: 0 42 313 417 735 1332 1407
Size: 42 271 104 318 597 75
Fragments arranged by size:
597 318 271 104 75 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 686 721 1407
Size: 686 35 686
Fragments arranged by size:
686 686 35
DraI TTT'AAA
Cuts at: 0 572 1407
Size: 572 835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1043 1055 1145 1297 1407
Size: 971 72 12 90 152 110
Fragments arranged by size:
971 152 110 90 72 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 640 672 1407
Size: 292 32 316 32 735
Fragments arranged by size:
735 316 292 32 32
HincII GTy'rAC
Cuts at: 0 1121 1407
Size: 1121 286
HindIII A'AGCT_T
Cuts at: 0 661 1407
Size: 661 746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1407
Size: 786 621
PsiI TTA'TAA
Cuts at: 0 1105 1407
Size: 1105 302
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 1407
Size: 1049 32 326
Fragments arranged by size:
1049 326 32
PstI C_TGCA'G
Cuts at: 0 42 1407
Size: 42 1365
SfcI C'TryA_G
Cuts at: 0 38 461 854 1190 1407
Size: 38 423 393 336 217
Fragments arranged by size:
423 393 336 217 38
SmaI CCC'GGG
Cuts at: 0 205 1407
Size: 205 1202
SmlI C'TyrA_G
Cuts at: 0 54 1051 1407
Size: 54 997 356
Fragments arranged by size:
997 356 54
SspI AAT'ATT
Cuts at: 0 523 1012 1407
Size: 523 489 395
Fragments arranged by size:
523 489 395
StyI C'CwwG_G
Cuts at: 0 170 1407
Size: 170 1237
SwaI ATTT'AAAT
Cuts at: 0 572 1407
Size: 572 835
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 216 1407
Size: 216 1191
TatI w'GTAC_w
Cuts at: 0 1132 1407
Size: 1132 275
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 587 619 1407
Size: 254 32 301 32 788
Fragments arranged by size:
788 301 254 32 32
XmaI C'CCGG_G
Cuts at: 0 203 1407
Size: 203 1204
XmnI GAAnn'nnTTC
Cuts at: 0 1298 1314 1407
Size: 1298 16 93
Fragments arranged by size:
1298 93 16
Enzymes that do cut:
AccI AcuI AflII AlwNI ApoI AseI AvaI BanII
BbsI BciVI BglII BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrBI
BsrDI BstYI CspCI DraI Eco57MI Hin4I HincII HindIII
MmeI PsiI PsrI PstI SfcI SmaI SmlI SspI
StyI SwaI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
Tth111I XbaI XcmI XhoI ZraI