Restriction map for
pFN21AE1310
(Linear) (Six-base) MAPSORT of: KIEE1310.seq Check: 8261 from: 1 to: 1191
KIEE1310.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 893 1191
Size: 746 147 298
Fragments arranged by size:
746 298 147
AleI CACnn'nnGTG
Cuts at: 0 80 1191
Size: 80 1111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 614 833 1191
Size: 614 219 358
Fragments arranged by size:
614 358 219
AlwNI CAG_nnn'CTG
Cuts at: 0 264 626 969 1191
Size: 264 362 343 222
Fragments arranged by size:
362 343 264 222
ApaI G_GGCC'C
Cuts at: 0 450 620 1191
Size: 450 170 571
Fragments arranged by size:
571 450 170
BanI G'GyrC_C
Cuts at: 0 301 1182 1191
Size: 301 881 9
Fragments arranged by size:
881 301 9
BanII G_rGCy'C
Cuts at: 0 386 450 620 760 1191
Size: 386 64 170 140 431
Fragments arranged by size:
431 386 170 140 64
BbvCI CC'TCA_GC
Cuts at: 0 632 932 1191
Size: 632 300 259
Fragments arranged by size:
632 300 259
BglI GCCn_nnn'nGGC
Cuts at: 0 1097 1191
Size: 1097 94
Bme1580I G_kGCm'C
Cuts at: 0 450 547 620 1187 1191
Size: 450 97 73 567 4
Fragments arranged by size:
567 450 97 73 4
BmgBI CAC'GTC
Cuts at: 0 855 1191
Size: 855 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 840 1191
Size: 840 351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 617 1191
Size: 617 574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 433 799 1078 1191
Size: 400 33 366 279 113
Fragments arranged by size:
400 366 279 113 33
Bpu10I CC'TnA_GC
Cuts at: 0 524 632 932 1191
Size: 524 108 300 259
Fragments arranged by size:
524 300 259 108
BsaAI yAC'GTr
Cuts at: 0 104 587 1191
Size: 104 483 604
Fragments arranged by size:
604 483 104
BsaHI Gr'CG_yC
Cuts at: 0 514 974 998 1191
Size: 514 460 24 193
Fragments arranged by size:
514 460 193 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 524 554 1146 1176 1191
Size: 524 30 592 30 15
Fragments arranged by size:
592 524 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 724 802 864 1042 1191
Size: 724 78 62 178 149
Fragments arranged by size:
724 178 149 78 62
BseYI C'CCAG_C
Cuts at: 0 619 699 889 969 1191
Size: 619 80 190 80 222
Fragments arranged by size:
619 222 190 80 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 1191
Size: 126 1065
BsmI GAATG_Cn'
Cuts at: 0 720 1191
Size: 720 471
BsmBI CGTCTCn'nnnn_
Cuts at: 0 190 576 861 1191
Size: 190 386 285 330
Fragments arranged by size:
386 330 285 190
Bsp1286I G_dGCh'C
Cuts at: 0 386 450 547 620 760 1187 1191
Size: 386 64 97 73 140 427 4
Fragments arranged by size:
427 386 140 97 73 64 4
BsrBI CCG'CTC
Cuts at: 0 783 1191
Size: 783 408
BsrDI GCAATG_nn'
Cuts at: 0 244 1191
Size: 244 947
BstXI CCAn_nnnn'nTGG
Cuts at: 0 613 1191
Size: 613 578
Bsu36I CC'TnA_GG
Cuts at: 0 492 926 1191
Size: 492 434 265
Fragments arranged by size:
492 434 265
BtgI C'CryG_G
Cuts at: 0 78 953 1191
Size: 78 875 238
Fragments arranged by size:
875 238 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 268 1191
Size: 268 923
BtsI GCAGTG_nn'
Cuts at: 0 575 686 1191
Size: 575 111 505
Fragments arranged by size:
575 505 111
DraIII CAC_nnn'GTG
Cuts at: 0 84 1191
Size: 84 1107
EaeI y'GGCC_r
Cuts at: 0 35 1070 1191
Size: 35 1035 121
Fragments arranged by size:
1035 121 35
EarI CTCTTCn'nnn_
Cuts at: 0 1060 1191
Size: 1060 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1144 1191
Size: 1144 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 433 746 799 893 1078 1191
Size: 400 33 313 53 94 185 113
Fragments arranged by size:
400 313 185 113 94 53 33
EcoO109I rG'GnC_Cy
Cuts at: 0 446 447 455 535 651 666 796 835
Size: 446 1 8 80 116 15 130 39
Cuts at: 835 884 1134 1191
Size: 49 250 57
Fragments arranged by size:
446 250 130 116 80 57 49 39
15 8 1
EcoRV GAT'ATC
Cuts at: 0 11 1191
Size: 11 1180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 640 672 1191
Size: 246 32 362 32 519
Fragments arranged by size:
519 362 246 32 32
MscI TGG'CCA
Cuts at: 0 37 1072 1191
Size: 37 1035 119
Fragments arranged by size:
1035 119 37
MslI CAynn'nnrTG
Cuts at: 0 80 815 1191
Size: 80 735 376
Fragments arranged by size:
735 376 80
MspA1I CmG'CkG
Cuts at: 0 917 1191
Size: 917 274
NcoI C'CATG_G
Cuts at: 0 953 1191
Size: 953 238
NdeI CA'TA_TG
Cuts at: 0 1173 1191
Size: 1173 18
PasI CC'CwG_GG
Cuts at: 0 450 1191
Size: 450 741
PflMI CCAn_nnn'nTGG
Cuts at: 0 626 969 1191
Size: 626 343 222
Fragments arranged by size:
626 343 222
PfoI T'CCnGG_A
Cuts at: 0 377 824 1191
Size: 377 447 367
Fragments arranged by size:
447 377 367
PmlI CAC'GTG
Cuts at: 0 104 587 1191
Size: 104 483 604
Fragments arranged by size:
604 483 104
PpuMI rG'GwC_Cy
Cuts at: 0 455 666 796 884 1191
Size: 455 211 130 88 307
Fragments arranged by size:
455 307 211 130 88
PspOMI G'GGCC_C
Cuts at: 0 446 616 1191
Size: 446 170 575
Fragments arranged by size:
575 446 170
PvuII CAG'CTG
Cuts at: 0 917 1191
Size: 917 274
SanDI GG'GwC_CC
Cuts at: 0 796 884 1191
Size: 796 88 307
Fragments arranged by size:
796 307 88
SfcI C'TryA_G
Cuts at: 0 131 1191
Size: 131 1060
StuI AGG'CCT
Cuts at: 0 682 1191
Size: 682 509
StyI C'CwwG_G
Cuts at: 0 953 1044 1191
Size: 953 91 147
Fragments arranged by size:
953 147 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 691 1191
Size: 676 15 500
Fragments arranged by size:
676 500 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 524 556 1191
Size: 524 32 635
Fragments arranged by size:
635 524 32
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI BanI BanII BbvCI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I
BsrBI BsrDI BstXI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoO109I EcoRV Hin4I MscI
MslI MspA1I NcoI NdeI PasI PflMI PfoI PmlI
PpuMI PspOMI PvuII SanDI SfcI StuI StyI TaqII
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BpuEI BsaI BsaBI
BsaWI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI