Restriction map for
pFN21AE1309
(Linear) (Six-base) MAPSORT of: KIEE1309.seq Check: 2900 from: 1 to: 1071
KIEE1309.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AccI GT'mk_AC
Cuts at: 0 371 1071
Size: 371 700
Acc65I G'GTAC_C
Cuts at: 0 15 1071
Size: 15 1056
AflIII A'CryG_T
Cuts at: 0 716 1071
Size: 716 355
AlwNI CAG_nnn'CTG
Cuts at: 0 145 160 1071
Size: 145 15 911
Fragments arranged by size:
911 145 15
ApaI G_GGCC'C
Cuts at: 0 254 1071
Size: 254 817
ApoI r'AATT_y
Cuts at: 0 451 1071
Size: 451 620
AvaI C'yCGr_G
Cuts at: 0 341 1071
Size: 341 730
BanI G'GyrC_C
Cuts at: 0 15 1071
Size: 15 1056
BanII G_rGCy'C
Cuts at: 0 254 503 1071
Size: 254 249 568
Fragments arranged by size:
568 254 249
BbsI GAAGACnn'nnnn_
Cuts at: 0 766 1071
Size: 766 305
BbvCI CC'TCA_GC
Cuts at: 0 192 1071
Size: 192 879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 353 1071
Size: 319 34 718
Fragments arranged by size:
718 319 34
BclI T'GATC_A
Cuts at: 0 752 1071
Size: 752 319
BglI GCCn_nnn'nGGC
Cuts at: 0 10 1071
Size: 10 1061
Bme1580I G_kGCm'C
Cuts at: 0 254 1071
Size: 254 817
BmrI ACTGGGnnnn_n'
Cuts at: 0 43 280 1071
Size: 43 237 791
Fragments arranged by size:
791 237 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 188 601 1071
Size: 110 78 413 470
Fragments arranged by size:
470 413 110 78
Bpu10I CC'TnA_GC
Cuts at: 0 192 1071
Size: 192 879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1071
Size: 780 291
BsaI GGTCTCn'nnnn_
Cuts at: 0 964 1071
Size: 964 107
BsaWI w'CCGG_w
Cuts at: 0 108 1071
Size: 108 963
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 186 1071
Size: 156 30 885
Fragments arranged by size:
885 156 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 1071
Size: 20 1051
BseYI C'CCAG_C
Cuts at: 0 66 216 1071
Size: 66 150 855
Fragments arranged by size:
855 150 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1071
Size: 265 806
BsiEI CG_ry'CG
Cuts at: 0 267 627 1071
Size: 267 360 444
Fragments arranged by size:
444 360 267
BsiHKAI G_wGCw'C
Cuts at: 0 30 503 545 1071
Size: 30 473 42 526
Fragments arranged by size:
526 473 42 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 315 1071
Size: 315 756
Bsp1286I G_dGCh'C
Cuts at: 0 30 254 503 545 1071
Size: 30 224 249 42 526
Fragments arranged by size:
526 249 224 42 30
BspHI T'CATG_A
Cuts at: 0 755 1071
Size: 755 316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 772 1071
Size: 772 299
BsrBI CCG'CTC
Cuts at: 0 226 1071
Size: 226 845
BsrFI r'CCGG_y
Cuts at: 0 9 96 1071
Size: 9 87 975
Fragments arranged by size:
975 87 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 100 160 549 1071
Size: 100 60 389 522
Fragments arranged by size:
522 389 100 60
BstEII G'GTnAC_C
Cuts at: 0 390 1008 1071
Size: 390 618 63
Fragments arranged by size:
618 390 63
BtgI C'CryG_G
Cuts at: 0 680 1071
Size: 680 391
DraIII CAC_nnn'GTG
Cuts at: 0 384 549 1071
Size: 384 165 522
Fragments arranged by size:
522 384 165
EaeI y'GGCC_r
Cuts at: 0 2 35 177 264 624 1019 1071
Size: 2 33 142 87 360 395 52
Fragments arranged by size:
395 360 142 87 52 33 2
EagI C'GGCC_G
Cuts at: 0 264 624 1071
Size: 264 360 447
Fragments arranged by size:
447 360 264
EarI CTCTTCn'nnn_
Cuts at: 0 248 1071
Size: 248 823
EcoICRI GAG'CTC
Cuts at: 0 501 1071
Size: 501 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 188 601 1071
Size: 110 78 413 470
Fragments arranged by size:
470 413 110 78
EcoO109I rG'GnC_Cy
Cuts at: 0 250 251 353 579 1071
Size: 250 1 102 226 492
Fragments arranged by size:
492 250 226 102 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 1071
Size: 783 288
HaeII r_GCGC'y
Cuts at: 0 85 223 1071
Size: 85 138 848
Fragments arranged by size:
848 138 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 865 897 1071
Size: 364 32 469 32 174
Fragments arranged by size:
469 364 174 32 32
HincII GTy'rAC
Cuts at: 0 372 444 747 936 1071
Size: 372 72 303 189 135
Fragments arranged by size:
372 303 189 135 72
HpaI GTT'AAC
Cuts at: 0 936 1071
Size: 936 135
KpnI G_GTAC'C
Cuts at: 0 19 1071
Size: 19 1052
MfeI C'AATT_G
Cuts at: 0 396 1071
Size: 396 675
MluI A'CGCG_T
Cuts at: 0 716 1071
Size: 716 355
MscI TGG'CCA
Cuts at: 0 4 179 1021 1071
Size: 4 175 842 50
Fragments arranged by size:
842 175 50 4
MslI CAynn'nnrTG
Cuts at: 0 658 685 1071
Size: 658 27 386
Fragments arranged by size:
658 386 27
MspA1I CmG'CkG
Cuts at: 0 118 157 898 1071
Size: 118 39 741 173
Fragments arranged by size:
741 173 118 39
NcoI C'CATG_G
Cuts at: 0 680 1071
Size: 680 391
NotI GC'GGCC_GC
Cuts at: 0 264 1071
Size: 264 807
PasI CC'CwG_GG
Cuts at: 0 670 1071
Size: 670 401
PfoI T'CCnGG_A
Cuts at: 0 836 1071
Size: 836 235
PpuMI rG'GwC_Cy
Cuts at: 0 353 1071
Size: 353 718
PshAI GACnn'nnGTC
Cuts at: 0 611 1071
Size: 611 460
PspOMI G'GGCC_C
Cuts at: 0 250 1071
Size: 250 821
PvuII CAG'CTG
Cuts at: 0 898 1071
Size: 898 173
SacI G_AGCT'C
Cuts at: 0 503 1071
Size: 503 568
SalI G'TCGA_C
Cuts at: 0 370 1071
Size: 370 701
SanDI GG'GwC_CC
Cuts at: 0 353 1071
Size: 353 718
SapI GCTCTTCn'nnn_
Cuts at: 0 248 1071
Size: 248 823
ScaI AGT'ACT
Cuts at: 0 565 1071
Size: 565 506
SfcI C'TryA_G
Cuts at: 0 567 1071
Size: 567 504
SgrAI Cr'CCGG_yG
Cuts at: 0 9 1071
Size: 9 1062
SmlI C'TyrA_G
Cuts at: 0 795 1071
Size: 795 276
StyI C'CwwG_G
Cuts at: 0 680 1071
Size: 680 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 1071
Size: 299 772
TatI w'GTAC_w
Cuts at: 0 563 850 876 1071
Size: 563 287 26 195
Fragments arranged by size:
563 287 195 26
Enzymes that do cut:
AccI Acc65I AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BstAPI BstEII BtgI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI
KpnI MfeI MluI MscI MslI MspA1I NcoI NotI
PasI PfoI PpuMI PshAI PspOMI PvuII SacI SalI
SanDI SapI ScaI SfcI SgrAI SmlI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BglII BlpI BmgBI
BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmI BspEI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KasI
MmeI NaeI NarI NdeI NgoMIV NheI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI PstI PvuI RsrII SacII SbfI SexAI
SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI