(Linear) (Six-base) MAPSORT of: KIEE1304.seq  Check: 9020  from: 1  to: 867



KIEE1304.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     832     864     867 
   Size:        832      32       3

  Fragments arranged by size: 

                 832      32       3
 
ApoI r'AATT_y

Cuts at:      0     246     625     867 
   Size:        246     379     242

  Fragments arranged by size: 

                 379     246     242
 
AseI AT'TA_AT

Cuts at:      0     476     867 
   Size:        476     391
 
BanI G'GyrC_C

Cuts at:      0     283     867 
   Size:        283     584
 
BanII G_rGCy'C

Cuts at:      0     751     867 
   Size:        751     116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      48     311     867 
   Size:         48     263     556

  Fragments arranged by size: 

                 556     263      48
 
Bme1580I G_kGCm'C

Cuts at:      0     317     867 
   Size:        317     550
 
BmgBI CAC'GTC

Cuts at:      0     837     867 
   Size:        837      30
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      76     867 
   Size:         76     791
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     867 
   Size:         64     803
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     867 
   Size:         34     833
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     804     867 
   Size:        804      63
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     751     867 
   Size:        317     434     116

  Fragments arranged by size: 

                 434     317     116
 
BspHI T'CATG_A

Cuts at:      0      96     669     867 
   Size:         96     573     198

  Fragments arranged by size: 

                 573     198      96
 
BsrBI CCG'CTC

Cuts at:      0       5     867 
   Size:          5     862
 
BsrDI GCAATG_nn'

Cuts at:      0     467     867 
   Size:        467     400
 
BssHII G'CGCG_C

Cuts at:      0      24     867 
   Size:         24     843
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     480     867 
   Size:        480     387
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     818     853     867 
   Size:        818      35      14

  Fragments arranged by size: 

                 818      35      14
 
DraI TTT'AAA

Cuts at:      0     197     630     867 
   Size:        197     433     237

  Fragments arranged by size: 

                 433     237     197
 
DraIII CAC_nnn'GTG

Cuts at:      0     808     867 
   Size:        808      59
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     463     867 
   Size:        463     404
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     867 
   Size:         64     803
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     867 
   Size:        189     678
 
EcoRV GAT'ATC

Cuts at:      0     513     867 
   Size:        513     354
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     247     867 
   Size:        247     620
 
MfeI C'AATT_G

Cuts at:      0     356     867 
   Size:        356     511
 
MslI CAynn'nnrTG

Cuts at:      0     478     867 
   Size:        478     389
 
PasI CC'CwG_GG

Cuts at:      0     310     867 
   Size:        310     557
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     867 
   Size:        189     678
 
PsiI TTA'TAA

Cuts at:      0     506     867 
   Size:        506     361
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     267     299     867 
   Size:        267      32     568

  Fragments arranged by size: 

                 568     267      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     783     867 
   Size:        783      84
 
XmnI GAAnn'nnTTC

Cuts at:      0     250     867 
   Size:        250     617
 

 Enzymes that do cut:

     AloI     ApoI     AseI     BanI    BanII     BbsI Bme1580I    BmgBI
     BplI     BpmI    BseRI     BsgI Bsp1286I    BspHI    BsrBI    BsrDI
   BssHII    BstXI    CspCI     DraI   DraIII     DrdI  Eco57MI EcoO109I
    EcoRV     FalI     MfeI     MslI     PasI    PpuMI     PsiI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI
     XmaI     ZraI