Restriction map for
pFN21AE1300
(Linear) (Six-base) MAPSORT of: KIEE1300.seq Check: 9365 from: 1 to: 1716
KIEE1300.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AatII G_ACGT'C
Cuts at: 0 74 1716
Size: 74 1642
Acc65I G'GTAC_C
Cuts at: 0 628 1716
Size: 628 1088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1337 1716
Size: 1175 162 379
Fragments arranged by size:
1175 379 162
AflIII A'CryG_T
Cuts at: 0 20 1716
Size: 20 1696
AlwNI CAG_nnn'CTG
Cuts at: 0 138 1716
Size: 138 1578
ApaI G_GGCC'C
Cuts at: 0 664 922 1390 1716
Size: 664 258 468 326
Fragments arranged by size:
664 468 326 258
ApaLI G'TGCA_C
Cuts at: 0 202 1716
Size: 202 1514
AvaI C'yCGr_G
Cuts at: 0 105 153 298 321 1693 1716
Size: 105 48 145 23 1372 23
Fragments arranged by size:
1372 145 105 48 23 23
BanI G'GyrC_C
Cuts at: 0 401 538 628 645 1559 1716
Size: 401 137 90 17 914 157
Fragments arranged by size:
914 401 157 137 90 17
BanII G_rGCy'C
Cuts at: 0 152 664 733 854 883 922 1012 1067
Size: 152 512 69 121 29 39 90 55
Cuts at: 1067 1390 1619 1716
Size: 323 229 97
Fragments arranged by size:
512 323 229 152 121 97 90 69
55 39 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1425 1580 1653 1716
Size: 1425 155 73 63
Fragments arranged by size:
1425 155 73 63
BbvCI CC'TCA_GC
Cuts at: 0 340 656 1145 1528 1716
Size: 340 316 489 383 188
Fragments arranged by size:
489 383 340 316 188
BglI GCCn_nnn'nGGC
Cuts at: 0 119 827 899 1484 1716
Size: 119 708 72 585 232
Fragments arranged by size:
708 585 232 119 72
Bme1580I G_kGCm'C
Cuts at: 0 206 292 650 664 922 1390 1716
Size: 206 86 358 14 258 468 326
Fragments arranged by size:
468 358 326 258 206 86 14
BmgBI CAC'GTC
Cuts at: 0 207 1716
Size: 207 1509
BmrI ACTGGGnnnn_n'
Cuts at: 0 1189 1716
Size: 1189 527
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 1716
Size: 820 896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 445 504 531 1390 1716
Size: 145 300 59 27 859 326
Fragments arranged by size:
859 326 300 145 59 27
Bpu10I CC'TnA_GC
Cuts at: 0 89 340 656 1145 1528 1716
Size: 89 251 316 489 383 188
Fragments arranged by size:
489 383 316 251 188 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1716
Size: 838 878
BsaI GGTCTCn'nnnn_
Cuts at: 0 791 1213 1716
Size: 791 422 503
Fragments arranged by size:
791 503 422
BsaBI GATnn'nnATC
Cuts at: 0 262 1716
Size: 262 1454
BsaHI Gr'CG_yC
Cuts at: 0 71 1208 1361 1716
Size: 71 1137 153 355
Fragments arranged by size:
1137 355 153 71
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 1017 1716
Size: 814 203 699
Fragments arranged by size:
814 699 203
BseYI C'CCAG_C
Cuts at: 0 10 393 444 914 1716
Size: 10 383 51 470 802
Fragments arranged by size:
802 470 383 51 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1716
Size: 300 1416
BsiHKAI G_wGCw'C
Cuts at: 0 95 206 854 1716
Size: 95 111 648 862
Fragments arranged by size:
862 648 111 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 549 1716
Size: 549 1167
Bsp1286I G_dGCh'C
Cuts at: 0 95 152 206 292 650 664 733 854
Size: 95 57 54 86 358 14 69 121
Cuts at: 854 883 922 1012 1067 1390 1619 1716
Size: 29 39 90 55 323 229 97
Fragments arranged by size:
358 323 229 121 97 95 90 86
69 57 55 54 39 29 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 1716
Size: 146 1570
BsrDI GCAATG_nn'
Cuts at: 0 642 1494 1716
Size: 642 852 222
Fragments arranged by size:
852 642 222
BstAPI GCAn_nnn'nTGC
Cuts at: 0 287 363 1493 1716
Size: 287 76 1130 223
Fragments arranged by size:
1130 287 223 76
BstEII G'GTnAC_C
Cuts at: 0 1344 1716
Size: 1344 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1489 1716
Size: 1489 227
BstYI r'GATC_y
Cuts at: 0 1634 1697 1716
Size: 1634 63 19
Fragments arranged by size:
1634 63 19
BtgI C'CryG_G
Cuts at: 0 47 518 810 1349 1716
Size: 47 471 292 539 367
Fragments arranged by size:
539 471 367 292 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 588 636 1716
Size: 588 48 1080
Fragments arranged by size:
1080 588 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1212 1247 1440 1475 1716
Size: 1212 35 193 35 241
Fragments arranged by size:
1212 241 193 35 35
EaeI y'GGCC_r
Cuts at: 0 44 50 723 758 788 995 1082 1179
Size: 44 6 673 35 30 207 87 97
Cuts at: 1179 1461 1587 1716
Size: 282 126 129
Fragments arranged by size:
673 282 207 129 126 97 87 44
35 30 6
EarI CTCTTCn'nnn_
Cuts at: 0 217 811 871 1513 1716
Size: 217 594 60 642 203
Fragments arranged by size:
642 594 217 203 60
EcoICRI GAG'CTC
Cuts at: 0 852 1716
Size: 852 864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 445 504 531 1175 1337 1390 1716
Size: 145 300 59 27 644 162 53 326
Fragments arranged by size:
644 326 300 162 145 59 53 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 586 1599 1716
Size: 463 123 1013 117
Fragments arranged by size:
1013 463 123 117
EcoO109I rG'GnC_Cy
Cuts at: 0 182 317 468 660 661 680 711 918
Size: 182 135 151 192 1 19 31 207
Cuts at: 918 919 1185 1386 1387 1716
Size: 1 266 201 1 329
Fragments arranged by size:
329 266 207 201 192 182 151 135
31 19 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1276 1716
Size: 1276 440
FspI TGC'GCA
Cuts at: 0 262 1716
Size: 262 1454
FspAI rTGC'GCAy
Cuts at: 0 262 1716
Size: 262 1454
HaeII r_GCGC'y
Cuts at: 0 491 1584 1716
Size: 491 1093 132
Fragments arranged by size:
1093 491 132
KpnI G_GTAC'C
Cuts at: 0 632 1716
Size: 632 1084
MscI TGG'CCA
Cuts at: 0 46 760 790 1084 1463 1716
Size: 46 714 30 294 379 253
Fragments arranged by size:
714 379 294 253 46 30
MslI CAynn'nnrTG
Cuts at: 0 1651 1716
Size: 1651 65
MspA1I CmG'CkG
Cuts at: 0 276 561 704 1001 1351 1527 1553 1716
Size: 276 285 143 297 350 176 26 163
Fragments arranged by size:
350 297 285 276 176 163 143 26
NcoI C'CATG_G
Cuts at: 0 47 1716
Size: 47 1669
NspI r_CATG'y
Cuts at: 0 24 1680 1716
Size: 24 1656 36
Fragments arranged by size:
1656 36 24
PasI CC'CwG_GG
Cuts at: 0 407 909 1482 1716
Size: 407 502 573 234
Fragments arranged by size:
573 502 407 234
PciI A'CATG_T
Cuts at: 0 20 1716
Size: 20 1696
PflMI CCAn_nnn'nTGG
Cuts at: 0 524 1716
Size: 524 1192
PpuMI rG'GwC_Cy
Cuts at: 0 182 1716
Size: 182 1534
PspOMI G'GGCC_C
Cuts at: 0 660 918 1386 1716
Size: 660 258 468 330
Fragments arranged by size:
660 468 330 258
PstI C_TGCA'G
Cuts at: 0 368 892 1716
Size: 368 524 824
Fragments arranged by size:
824 524 368
PvuII CAG'CTG
Cuts at: 0 704 1527 1716
Size: 704 823 189
Fragments arranged by size:
823 704 189
SacI G_AGCT'C
Cuts at: 0 854 1716
Size: 854 862
SacII CC_GC'GG
Cuts at: 0 1352 1716
Size: 1352 364
SbfI CC_TGCA'GG
Cuts at: 0 368 1716
Size: 368 1348
SexAI A'CCwGG_T
Cuts at: 0 641 1716
Size: 641 1075
SfcI C'TryA_G
Cuts at: 0 364 888 1716
Size: 364 524 828
Fragments arranged by size:
828 524 364
SmaI CCC'GGG
Cuts at: 0 107 300 323 1695 1716
Size: 107 193 23 1372 21
Fragments arranged by size:
1372 193 107 23 21
SmlI C'TyrA_G
Cuts at: 0 153 853 1716
Size: 153 700 863
Fragments arranged by size:
863 700 153
StuI AGG'CCT
Cuts at: 0 415 436 1096 1716
Size: 415 21 660 620
Fragments arranged by size:
660 620 415 21
StyI C'CwwG_G
Cuts at: 0 47 582 1287 1503 1716
Size: 47 535 705 216 213
Fragments arranged by size:
705 535 216 213 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1547 1629 1716
Size: 1547 82 87
Fragments arranged by size:
1547 87 82
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 479 1716
Size: 479 1237
XhoI C'TCGA_G
Cuts at: 0 153 1716
Size: 153 1563
XmaI C'CCGG_G
Cuts at: 0 105 298 321 1693 1716
Size: 105 193 23 1372 23
Fragments arranged by size:
1372 193 105 23 23
ZraI GAC'GTC
Cuts at: 0 72 1716
Size: 72 1644
Enzymes that do cut:
AatII Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstAPI
BstEII BstXI BstYI BtgI BtgZI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
KpnI MscI MslI MspA1I NcoI NspI PasI PciI
PflMI PpuMI PspOMI PstI PvuII SacI SacII SbfI
SexAI SfcI SmaI SmlI StuI StyI TaqII XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BsaAI BsaWI BsaXI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtsI ClaI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XmnI