(Linear) (Six-base) MAPSORT of: KIEE1298.seq  Check: 961  from: 1  to: 408



KIEE1298.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:32  ..

AccI GT'mk_AC

Cuts at:      0     172     408 
   Size:        172     236
 
AfeI AGC'GCT

Cuts at:      0      87     408 
   Size:         87     321
 
BanII G_rGCy'C

Cuts at:      0     262     408 
   Size:        262     146
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38     162     196     408 
   Size:          4      34     124      34     212

  Fragments arranged by size: 

                 212     124      34      34       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     292     408 
   Size:         70     222     116

  Fragments arranged by size: 

                 222     116      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     408 
   Size:         74     334
 
BsiEI CG_ry'CG

Cuts at:      0     176     408 
   Size:        176     232
 
BsiHKAI G_wGCw'C

Cuts at:      0     262     408 
   Size:        262     146
 
BsmI GAATG_Cn'

Cuts at:      0     389     408 
   Size:        389      19
 
Bsp1286I G_dGCh'C

Cuts at:      0     262     408 
   Size:        262     146
 
BsrFI r'CCGG_y

Cuts at:      0      97     125     408 
   Size:         97      28     283

  Fragments arranged by size: 

                 283      97      28
 
BstYI r'GATC_y

Cuts at:      0     152     408 
   Size:        152     256
 
BtgI C'CryG_G

Cuts at:      0      32     408 
   Size:         32     376
 
EaeI y'GGCC_r

Cuts at:      0      62     408 
   Size:         62     346
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     408 
   Size:        145     263
 
EcoICRI GAG'CTC

Cuts at:      0     260     408 
   Size:        260     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     408 
   Size:         74     334
 
HaeII r_GCGC'y

Cuts at:      0      49      89     277     408 
   Size:         49      40     188     131

  Fragments arranged by size: 

                 188     131      49      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     341     373     408 
   Size:        341      32      35

  Fragments arranged by size: 

                 341      35      32
 
HincII GTy'rAC

Cuts at:      0     173     408 
   Size:        173     235
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MspA1I CmG'CkG

Cuts at:      0      60     408 
   Size:         60     348
 
PstI C_TGCA'G

Cuts at:      0     281     408 
   Size:        281     127
 
PvuII CAG'CTG

Cuts at:      0      60     408 
   Size:         60     348
 
SacI G_AGCT'C

Cuts at:      0     262     408 
   Size:        262     146
 
SalI G'TCGA_C

Cuts at:      0     171     408 
   Size:        171     237
 
SexAI A'CCwGG_T

Cuts at:      0     299     408 
   Size:        299     109
 
SfcI C'TryA_G

Cuts at:      0     277     408 
   Size:        277     131
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     292     408 
   Size:        292     116
 
SgrAI Cr'CCGG_yG

Cuts at:      0      97     408 
   Size:         97     311
 
StuI AGG'CCT

Cuts at:      0     286     295     408 
   Size:        286       9     113

  Fragments arranged by size: 

                 286     113       9
 
StyI C'CwwG_G

Cuts at:      0      68     365     408 
   Size:         68     297      43

  Fragments arranged by size: 

                 297      68      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     408 
   Size:        190     218
 

 Enzymes that do cut:

     AccI     AfeI    BanII     BcgI     BglI     BpmI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BsrFI    BstYI     BtgI     EaeI     EciI  EcoICRI
  Eco57MI    HaeII    Hin4I   HincII     MscI   MspA1I     PstI    PvuII
     SacI     SalI    SexAI     SfcI     SfiI    SgrAI     StuI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI