Restriction map for
pFN21AE1294
(Linear) (Six-base) MAPSORT of: KIEE1294.seq Check: 7487 from: 1 to: 1425
KIEE1294.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AclI AA'CG_TT
Cuts at: 0 1391 1425
Size: 1391 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 436 1020 1049 1341 1425
Size: 412 24 584 29 292 84
Fragments arranged by size:
584 412 292 84 29 24
AfeI AGC'GCT
Cuts at: 0 220 467 649 997 1425
Size: 220 247 182 348 428
Fragments arranged by size:
428 348 247 220 182
AflIII A'CryG_T
Cuts at: 0 1301 1425
Size: 1301 124
AleI CACnn'nnGTG
Cuts at: 0 946 1425
Size: 946 479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1186 1425
Size: 1186 239
AlwNI CAG_nnn'CTG
Cuts at: 0 663 693 921 978 1196 1401 1425
Size: 663 30 228 57 218 205 24
Fragments arranged by size:
663 228 218 205 57 30 24
AvaI C'yCGr_G
Cuts at: 0 151 900 1425
Size: 151 749 525
Fragments arranged by size:
749 525 151
BanI G'GyrC_C
Cuts at: 0 136 186 1425
Size: 136 50 1239
Fragments arranged by size:
1239 136 50
BanII G_rGCy'C
Cuts at: 0 179 687 1208 1425
Size: 179 508 521 217
Fragments arranged by size:
521 508 217 179
BbeI G_GCGC'C
Cuts at: 0 140 190 1425
Size: 140 50 1235
Fragments arranged by size:
1235 140 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 806 1084 1245 1425
Size: 497 309 278 161 180
Fragments arranged by size:
497 309 278 180 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 331 365 1425
Size: 331 34 1060
Fragments arranged by size:
1060 331 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1124 1425
Size: 1124 301
Bme1580I G_kGCm'C
Cuts at: 0 907 1425
Size: 907 518
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 868 1065 1077 1425
Size: 123 745 197 12 348
Fragments arranged by size:
745 348 197 123 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1425
Size: 619 806
Bpu10I CC'TnA_GC
Cuts at: 0 43 1425
Size: 43 1382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 463 748 1425
Size: 181 282 285 677
Fragments arranged by size:
677 285 282 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 490 775 1425
Size: 490 285 650
Fragments arranged by size:
650 490 285
BsaHI Gr'CG_yC
Cuts at: 0 137 187 242 389 1046 1425
Size: 137 50 55 147 657 379
Fragments arranged by size:
657 379 147 137 55 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 1425
Size: 487 30 908
Fragments arranged by size:
908 487 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 1425
Size: 607 818
BseYI C'CCAG_C
Cuts at: 0 190 736 1020 1144 1425
Size: 190 546 284 124 281
Fragments arranged by size:
546 284 281 190 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 335 340 538 924 1182 1425
Size: 27 308 5 198 386 258 243
Fragments arranged by size:
386 308 258 243 198 27 5
BsiEI CG_ry'CG
Cuts at: 0 720 1425
Size: 720 705
BsiHKAI G_wGCw'C
Cuts at: 0 34 113 1425
Size: 34 79 1312
Fragments arranged by size:
1312 79 34
Bsp1286I G_dGCh'C
Cuts at: 0 34 113 179 687 907 1208 1425
Size: 34 79 66 508 220 301 217
Fragments arranged by size:
508 301 220 217 79 66 34
BsrBI CCG'CTC
Cuts at: 0 450 549 702 759 1425
Size: 450 99 153 57 666
Fragments arranged by size:
666 450 153 99 57
BsrFI r'CCGG_y
Cuts at: 0 158 376 557 719 1425
Size: 158 218 181 162 706
Fragments arranged by size:
706 218 181 162 158
BssHII G'CGCG_C
Cuts at: 0 256 320 1425
Size: 256 64 1105
Fragments arranged by size:
1105 256 64
BssSI C'ACGA_G
Cuts at: 0 358 1425
Size: 358 1067
BstAPI GCAn_nnn'nTGC
Cuts at: 0 117 561 1346 1425
Size: 117 444 785 79
Fragments arranged by size:
785 444 117 79
BstEII G'GTnAC_C
Cuts at: 0 372 1425
Size: 372 1053
BstXI CCAn_nnnn'nTGG
Cuts at: 0 213 1381 1425
Size: 213 1168 44
Fragments arranged by size:
1168 213 44
BstYI r'GATC_y
Cuts at: 0 689 1136 1425
Size: 689 447 289
Fragments arranged by size:
689 447 289
BtgI C'CryG_G
Cuts at: 0 1149 1425
Size: 1149 276
BtsI GCAGTG_nn'
Cuts at: 0 352 976 1425
Size: 352 624 449
Fragments arranged by size:
624 449 352
DraIII CAC_nnn'GTG
Cuts at: 0 117 876 1425
Size: 117 759 549
Fragments arranged by size:
759 549 117
EaeI y'GGCC_r
Cuts at: 0 203 1365 1425
Size: 203 1162 60
Fragments arranged by size:
1162 203 60
EarI CTCTTCn'nnn_
Cuts at: 0 307 1425
Size: 307 1118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 508 619 1087 1425
Size: 508 111 468 338
Fragments arranged by size:
508 468 338 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 436 619 1020 1049 1341 1425
Size: 412 24 183 401 29 292 84
Fragments arranged by size:
412 401 292 183 84 29 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 859 1425
Size: 859 566
EcoO109I rG'GnC_Cy
Cuts at: 0 57 432 826 1425
Size: 57 375 394 599
Fragments arranged by size:
599 394 375 57
FspI TGC'GCA
Cuts at: 0 667 1425
Size: 667 758
HaeII r_GCGC'y
Cuts at: 0 140 190 222 469 651 921 999 1196
Size: 140 50 32 247 182 270 78 197
Cuts at: 1196 1425
Size: 229
Fragments arranged by size:
270 247 229 197 182 140 78 50
32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 674 706 1288 1320 1425
Size: 487 32 155 32 582 32 105
Fragments arranged by size:
582 487 155 105 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1234 1425
Size: 1234 191
KasI G'GCGC_C
Cuts at: 0 136 186 1425
Size: 136 50 1239
Fragments arranged by size:
1239 136 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1425
Size: 911 514
MscI TGG'CCA
Cuts at: 0 205 1367 1425
Size: 205 1162 58
Fragments arranged by size:
1162 205 58
MslI CAynn'nnrTG
Cuts at: 0 946 1168 1379 1425
Size: 946 222 211 46
Fragments arranged by size:
946 222 211 46
MspA1I CmG'CkG
Cuts at: 0 98 237 474 663 1151 1401 1425
Size: 98 139 237 189 488 250 24
Fragments arranged by size:
488 250 237 189 139 98 24
NaeI GCC'GGC
Cuts at: 0 160 1425
Size: 160 1265
NarI GG'CG_CC
Cuts at: 0 137 187 1425
Size: 137 50 1238
Fragments arranged by size:
1238 137 50
NdeI CA'TA_TG
Cuts at: 0 795 1425
Size: 795 630
NgoMIV G'CCGG_C
Cuts at: 0 158 1425
Size: 158 1267
NspI r_CATG'y
Cuts at: 0 1305 1425
Size: 1305 120
PciI A'CATG_T
Cuts at: 0 1301 1425
Size: 1301 124
PfoI T'CCnGG_A
Cuts at: 0 674 860 1226 1256 1425
Size: 674 186 366 30 169
Fragments arranged by size:
674 366 186 169 30
PpuMI rG'GwC_Cy
Cuts at: 0 57 1425
Size: 57 1368
PstI C_TGCA'G
Cuts at: 0 407 473 698 836 983 1425
Size: 407 66 225 138 147 442
Fragments arranged by size:
442 407 225 147 138 66
PvuII CAG'CTG
Cuts at: 0 474 663 1425
Size: 474 189 762
Fragments arranged by size:
762 474 189
RsrII CG'GwC_CG
Cuts at: 0 315 1425
Size: 315 1110
SacII CC_GC'GG
Cuts at: 0 1152 1425
Size: 1152 273
SapI GCTCTTCn'nnn_
Cuts at: 0 307 1425
Size: 307 1118
SbfI CC_TGCA'GG
Cuts at: 0 698 1425
Size: 698 727
SfcI C'TryA_G
Cuts at: 0 222 403 469 651 694 832 979 1425
Size: 222 181 66 182 43 138 147 446
Fragments arranged by size:
446 222 182 181 147 138 66 43
SfoI GGC'GCC
Cuts at: 0 138 188 1425
Size: 138 50 1237
Fragments arranged by size:
1237 138 50
SmaI CCC'GGG
Cuts at: 0 902 1425
Size: 902 523
SmlI C'TyrA_G
Cuts at: 0 13 196 478 763 1425
Size: 13 183 282 285 662
Fragments arranged by size:
662 285 282 183 13
StuI AGG'CCT
Cuts at: 0 541 1425
Size: 541 884
StyI C'CwwG_G
Cuts at: 0 1269 1425
Size: 1269 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 105 640 887 983 1077 1237 1425
Size: 105 535 247 96 94 160 188
Fragments arranged by size:
535 247 188 160 105 96 94
TatI w'GTAC_w
Cuts at: 0 818 836 1187 1425
Size: 818 18 351 238
Fragments arranged by size:
818 351 238 18
Tth111I GACn'n_nGTC
Cuts at: 0 292 501 1425
Size: 292 209 924
Fragments arranged by size:
924 292 209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 1425
Size: 198 1227
XmaI C'CCGG_G
Cuts at: 0 900 1425
Size: 900 525
Enzymes that do cut:
AclI AcuI AfeI AflIII AleI AlfI AlwNI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PfoI PpuMI PstI PvuII
RsrII SacII SapI SbfI SfcI SfoI SmaI SmlI
StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HpaI KpnI MfeI
MluI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI SacI SalI SanDI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI XmnI ZraI