Restriction map for
pFN21AE1289
(Linear) (Six-base) MAPSORT of: KIEE1289.seq Check: 3368 from: 1 to: 2787
KIEE1289.orf
With 182 enzymes: SgfI *
March 22, 2012 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 414 2787
Size: 414 2373
AccI GT'mk_AC
Cuts at: 0 228 2787
Size: 228 2559
Acc65I G'GTAC_C
Cuts at: 0 2666 2787
Size: 2666 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 572 578 1016 1265 2787
Size: 304 268 6 438 249 1522
Fragments arranged by size:
1522 438 304 268 249 6
AfeI AGC'GCT
Cuts at: 0 118 2787
Size: 118 2669
AleI CACnn'nnGTG
Cuts at: 0 106 2563 2787
Size: 106 2457 224
Fragments arranged by size:
2457 224 106
AlwNI CAG_nnn'CTG
Cuts at: 0 60 89 132 2787
Size: 60 29 43 2655
Fragments arranged by size:
2655 60 43 29
ApaI G_GGCC'C
Cuts at: 0 2473 2787
Size: 2473 314
ApoI r'AATT_y
Cuts at: 0 284 519 1222 1274 1732 1780 2787
Size: 284 235 703 52 458 48 1007
Fragments arranged by size:
1007 703 458 284 235 52 48
AseI AT'TA_AT
Cuts at: 0 509 2787
Size: 509 2278
AvaI C'yCGr_G
Cuts at: 0 635 730 2259 2341 2490 2787
Size: 635 95 1529 82 149 297
Fragments arranged by size:
1529 635 297 149 95 82
BamHI G'GATC_C
Cuts at: 0 1339 2787
Size: 1339 1448
BanI G'GyrC_C
Cuts at: 0 426 717 2355 2666 2787
Size: 426 291 1638 311 121
Fragments arranged by size:
1638 426 311 291 121
BanII G_rGCy'C
Cuts at: 0 164 952 970 2473 2784 2787
Size: 164 788 18 1503 311 3
Fragments arranged by size:
1503 788 311 164 18 3
BbeI G_GCGC'C
Cuts at: 0 2359 2787
Size: 2359 428
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 389 850 2383 2787
Size: 248 141 461 1533 404
Fragments arranged by size:
1533 461 404 248 141
BciVI GTATCCnnnnn_n'
Cuts at: 0 924 1730 2787
Size: 924 806 1057
Fragments arranged by size:
1057 924 806
BglI GCCn_nnn'nGGC
Cuts at: 0 2344 2353 2509 2787
Size: 2344 9 156 278
Fragments arranged by size:
2344 278 156 9
BglII A'GATC_T
Cuts at: 0 47 2591 2787
Size: 47 2544 196
Fragments arranged by size:
2544 196 47
BlpI GC'TnA_GC
Cuts at: 0 82 1199 2787
Size: 82 1117 1588
Fragments arranged by size:
1588 1117 82
Bme1580I G_kGCm'C
Cuts at: 0 431 2473 2787
Size: 431 2042 314
Fragments arranged by size:
2042 431 314
BmgBI CAC'GTC
Cuts at: 0 108 2787
Size: 108 2679
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 1444 2787
Size: 861 583 1343
Fragments arranged by size:
1343 861 583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 661 2787
Size: 30 631 2126
Fragments arranged by size:
2126 631 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 734 818 1998 2787
Size: 671 63 84 1180 789
Fragments arranged by size:
1180 789 671 84 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1356 2787
Size: 386 970 1431
Fragments arranged by size:
1431 970 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 39 528 999 2787
Size: 39 489 471 1788
Fragments arranged by size:
1788 489 471 39
BsaAI yAC'GTr
Cuts at: 0 1423 2565 2787
Size: 1423 1142 222
Fragments arranged by size:
1423 1142 222
BsaBI GATnn'nnATC
Cuts at: 0 1239 1793 2317 2596 2787
Size: 1239 554 524 279 191
Fragments arranged by size:
1239 554 524 279 191
BsaHI Gr'CG_yC
Cuts at: 0 2356 2787
Size: 2356 431
BsaWI w'CCGG_w
Cuts at: 0 1360 2055 2787
Size: 1360 695 732
Fragments arranged by size:
1360 732 695
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 816 846 2787
Size: 816 30 1941
Fragments arranged by size:
1941 816 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 508 1337 1484 1543 2086 2787
Size: 88 420 829 147 59 543 701
Fragments arranged by size:
829 701 543 420 147 88 59
BseYI C'CCAG_C
Cuts at: 0 60 65 132 1102 1947 2022 2787
Size: 60 5 67 970 845 75 765
Fragments arranged by size:
970 845 765 75 67 60 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 2681 2787
Size: 97 2584 106
Fragments arranged by size:
2584 106 97
BsiHKAI G_wGCw'C
Cuts at: 0 164 2787
Size: 164 2623
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2542 2787
Size: 2542 245
Bsp1286I G_dGCh'C
Cuts at: 0 164 431 952 970 2473 2784 2787
Size: 164 267 521 18 1503 311 3
Fragments arranged by size:
1503 521 311 267 164 18 3
BspEI T'CCGG_A
Cuts at: 0 2055 2787
Size: 2055 732
BspHI T'CATG_A
Cuts at: 0 2690 2787
Size: 2690 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 389 414 453 2787
Size: 389 25 39 2334
Fragments arranged by size:
2334 389 39 25
BsrDI GCAATG_nn'
Cuts at: 0 884 2787
Size: 884 1903
BsrFI r'CCGG_y
Cuts at: 0 2352 2388 2787
Size: 2352 36 399
Fragments arranged by size:
2352 399 36
BsrGI T'GTAC_A
Cuts at: 0 696 2787
Size: 696 2091
BssSI C'ACGA_G
Cuts at: 0 1187 2787
Size: 1187 1600
BstBI TT'CG_AA
Cuts at: 0 1220 2787
Size: 1220 1567
BstYI r'GATC_y
Cuts at: 0 47 668 1212 1339 1755 2591 2597 2787
Size: 47 621 544 127 416 836 6 190
Fragments arranged by size:
836 621 544 416 190 127 47 6
BtgI C'CryG_G
Cuts at: 0 1553 2465 2787
Size: 1553 912 322
Fragments arranged by size:
1553 912 322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2672 2787
Size: 2672 115
BtsI GCAGTG_nn'
Cuts at: 0 2722 2787
Size: 2722 65
ClaI AT'CG_AT
Cuts at: 0 1172 2787
Size: 1172 1615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1946 1981 2787
Size: 1946 35 806
Fragments arranged by size:
1946 806 35
DraI TTT'AAA
Cuts at: 0 1770 2787
Size: 1770 1017
DraIII CAC_nnn'GTG
Cuts at: 0 1045 1521 2787
Size: 1045 476 1266
Fragments arranged by size:
1266 1045 476
DrdI GACnn_nn'nnGTC
Cuts at: 0 2314 2787
Size: 2314 473
EaeI y'GGCC_r
Cuts at: 0 168 2301 2787
Size: 168 2133 486
Fragments arranged by size:
2133 486 168
EarI CTCTTCn'nnn_
Cuts at: 0 413 1482 1742 2173 2188 2702 2761 2787
Size: 413 1069 260 431 15 514 59 26
Fragments arranged by size:
1069 514 431 413 260 59 26 15
EcoICRI GAG'CTC
Cuts at: 0 162 2787
Size: 162 2625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 572 578 671 734 818 1016 1265
Size: 304 268 6 93 63 84 198 249
Cuts at: 1265 1998 2787
Size: 733 789
Fragments arranged by size:
789 733 304 268 249 198 93 84
63 6
EcoO109I rG'GnC_Cy
Cuts at: 0 2019 2470 2494 2787
Size: 2019 451 24 293
Fragments arranged by size:
2019 451 293 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1837 2787
Size: 1837 950
FspI TGC'GCA
Cuts at: 0 2533 2787
Size: 2533 254
HaeII r_GCGC'y
Cuts at: 0 61 89 120 2359 2787
Size: 61 28 31 2239 428
Fragments arranged by size:
2239 428 61 31 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2270 2302 2760 2787
Size: 2270 32 458 27
Fragments arranged by size:
2270 458 32 27
HindIII A'AGCT_T
Cuts at: 0 203 1304 2787
Size: 203 1101 1483
Fragments arranged by size:
1483 1101 203
KasI G'GCGC_C
Cuts at: 0 2355 2787
Size: 2355 432
KpnI G_GTAC'C
Cuts at: 0 2670 2787
Size: 2670 117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 2664 2787
Size: 864 1800 123
Fragments arranged by size:
1800 864 123
MscI TGG'CCA
Cuts at: 0 2303 2787
Size: 2303 484
MslI CAynn'nnrTG
Cuts at: 0 106 2222 2563 2787
Size: 106 2116 341 224
Fragments arranged by size:
2116 341 224 106
MspA1I CmG'CkG
Cuts at: 0 132 213 2442 2448 2467 2787
Size: 132 81 2229 6 19 320
Fragments arranged by size:
2229 320 132 81 19 6
NaeI GCC'GGC
Cuts at: 0 2354 2390 2787
Size: 2354 36 397
Fragments arranged by size:
2354 397 36
NarI GG'CG_CC
Cuts at: 0 2356 2787
Size: 2356 431
NgoMIV G'CCGG_C
Cuts at: 0 2352 2388 2787
Size: 2352 36 399
Fragments arranged by size:
2352 399 36
PasI CC'CwG_GG
Cuts at: 0 2605 2787
Size: 2605 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 216 2787
Size: 60 156 2571
Fragments arranged by size:
2571 156 60
PfoI T'CCnGG_A
Cuts at: 0 834 2787
Size: 834 1953
PpuMI rG'GwC_Cy
Cuts at: 0 2019 2494 2787
Size: 2019 475 293
Fragments arranged by size:
2019 475 293
PspOMI G'GGCC_C
Cuts at: 0 2469 2787
Size: 2469 318
PstI C_TGCA'G
Cuts at: 0 116 128 2787
Size: 116 12 2659
Fragments arranged by size:
2659 116 12
PvuII CAG'CTG
Cuts at: 0 132 213 2787
Size: 132 81 2574
Fragments arranged by size:
2574 132 81
SacI G_AGCT'C
Cuts at: 0 164 2787
Size: 164 2623
SacII CC_GC'GG
Cuts at: 0 2468 2787
Size: 2468 319
SanDI GG'GwC_CC
Cuts at: 0 2019 2494 2787
Size: 2019 475 293
Fragments arranged by size:
2019 475 293
SapI GCTCTTCn'nnn_
Cuts at: 0 413 2787
Size: 413 2374
SfcI C'TryA_G
Cuts at: 0 112 124 2787
Size: 112 12 2663
Fragments arranged by size:
2663 112 12
SfoI GGC'GCC
Cuts at: 0 2357 2787
Size: 2357 430
SmaI CCC'GGG
Cuts at: 0 2492 2787
Size: 2492 295
SmlI C'TyrA_G
Cuts at: 0 401 635 1371 2787
Size: 401 234 736 1416
Fragments arranged by size:
1416 736 401 234
SnaBI TAC'GTA
Cuts at: 0 1423 2787
Size: 1423 1364
StuI AGG'CCT
Cuts at: 0 1165 2787
Size: 1165 1622
StyI C'CwwG_G
Cuts at: 0 470 971 2787
Size: 470 501 1816
Fragments arranged by size:
1816 501 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 402 2597 2633 2787
Size: 402 2195 36 154
Fragments arranged by size:
2195 402 154 36
TatI w'GTAC_w
Cuts at: 0 315 696 706 1291 2787
Size: 315 381 10 585 1496
Fragments arranged by size:
1496 585 381 315 10
XhoI C'TCGA_G
Cuts at: 0 635 2787
Size: 635 2152
XmaI C'CCGG_G
Cuts at: 0 2490 2787
Size: 2490 297
XmnI GAAnn'nnTTC
Cuts at: 0 368 1324 1443 2787
Size: 368 956 119 1344
Fragments arranged by size:
1344 956 368 119
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI SfcI SfoI SmaI
SmlI SnaBI StuI StyI TaqII TatI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI AloI
ApaLI AscI AsiSI AvrII BaeI BbvCI BcgI BclI
BfrBI BmtI Bpu10I BsiEI BsiWI BsmI BsrBI BssHII
BstAPI BstEII BstXI BstZ17I Bsu36I EagI EciI EcoNI
EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI ZraI