Restriction map for
pFN21AE1288
(Linear) (Six-base) MAPSORT of: KIEE1288.seq Check: 5110 from: 1 to: 2706
KIEE1288.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AccI GT'mk_AC
Cuts at: 0 2565 2706
Size: 2565 141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 254 395 2033 2398 2706
Size: 128 126 141 1638 365 308
Fragments arranged by size:
1638 365 308 141 128 126
AflIII A'CryG_T
Cuts at: 0 870 2448 2706
Size: 870 1578 258
Fragments arranged by size:
1578 870 258
AhdI GACnn_n'nnGTC
Cuts at: 0 423 2706
Size: 423 2283
AlwNI CAG_nnn'CTG
Cuts at: 0 166 331 718 2706
Size: 166 165 387 1988
Fragments arranged by size:
1988 387 166 165
ApaI G_GGCC'C
Cuts at: 0 908 2706
Size: 908 1798
ApoI r'AATT_y
Cuts at: 0 1295 1725 1738 2358 2706
Size: 1295 430 13 620 348
Fragments arranged by size:
1295 620 430 348 13
AvaI C'yCGr_G
Cuts at: 0 227 1537 2706
Size: 227 1310 1169
Fragments arranged by size:
1310 1169 227
AvrII C'CTAG_G
Cuts at: 0 247 2706
Size: 247 2459
BanI G'GyrC_C
Cuts at: 0 795 1586 2659 2706
Size: 795 791 1073 47
Fragments arranged by size:
1073 795 791 47
BanII G_rGCy'C
Cuts at: 0 908 2706
Size: 908 1798
BbsI GAAGACnn'nnnn_
Cuts at: 0 2168 2706
Size: 2168 538
BciVI GTATCCnnnnn_n'
Cuts at: 0 723 729 2706
Size: 723 6 1977
Fragments arranged by size:
1977 723 6
BfrBI ATG'CAT
Cuts at: 0 2323 2706
Size: 2323 383
BglII A'GATC_T
Cuts at: 0 370 2706
Size: 370 2336
BlpI GC'TnA_GC
Cuts at: 0 1544 2706
Size: 1544 1162
Bme1580I G_kGCm'C
Cuts at: 0 908 2662 2706
Size: 908 1754 44
Fragments arranged by size:
1754 908 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1618 2507 2706
Size: 1618 889 199
Fragments arranged by size:
1618 889 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1048 1097 1538 1872 2706
Size: 113 935 49 441 334 834
Fragments arranged by size:
935 834 441 334 113 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 150 2706
Size: 150 2556
BsaBI GATnn'nnATC
Cuts at: 0 141 1448 2706
Size: 141 1307 1258
Fragments arranged by size:
1307 1258 141
BsaWI w'CCGG_w
Cuts at: 0 1045 2706
Size: 1045 1661
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 453 958 1231 2706
Size: 453 505 273 1475
Fragments arranged by size:
1475 505 453 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 2706
Size: 838 1868
BsmI GAATG_Cn'
Cuts at: 0 2126 2706
Size: 2126 580
Bsp1286I G_dGCh'C
Cuts at: 0 908 2662 2706
Size: 908 1754 44
Fragments arranged by size:
1754 908 44
BspEI T'CCGG_A
Cuts at: 0 1045 2706
Size: 1045 1661
BspHI T'CATG_A
Cuts at: 0 2234 2706
Size: 2234 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 810 1806 2706
Size: 810 996 900
Fragments arranged by size:
996 900 810
BsrDI GCAATG_nn'
Cuts at: 0 95 2505 2706
Size: 95 2410 201
Fragments arranged by size:
2410 201 95
BssSI C'ACGA_G
Cuts at: 0 20 2706
Size: 20 2686
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1720 2706
Size: 1720 986
BstYI r'GATC_y
Cuts at: 0 370 1821 2706
Size: 370 1451 885
Fragments arranged by size:
1451 885 370
BtsI GCAGTG_nn'
Cuts at: 0 978 994 2306 2706
Size: 978 16 1312 400
Fragments arranged by size:
1312 978 400 16
DraI TTT'AAA
Cuts at: 0 212 347 1743 2391 2706
Size: 212 135 1396 648 315
Fragments arranged by size:
1396 648 315 212 135
DrdI GACnn_nn'nnGTC
Cuts at: 0 1811 2706
Size: 1811 895
EarI CTCTTCn'nnn_
Cuts at: 0 123 148 1430 1761 2706
Size: 123 25 1282 331 945
Fragments arranged by size:
1282 945 331 123 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 254 395 1618 2033 2398 2507 2706
Size: 128 126 141 1223 415 365 109 199
Fragments arranged by size:
1223 415 365 199 141 128 126 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1163 2706
Size: 1163 1543
EcoO109I rG'GnC_Cy
Cuts at: 0 904 905 2706
Size: 904 1 1801
Fragments arranged by size:
1801 904 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 577 2232 2706
Size: 577 1655 474
Fragments arranged by size:
1655 577 474
FspI TGC'GCA
Cuts at: 0 101 2706
Size: 101 2605
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 1404 1436 1825 1857 1860 1892
Size: 409 32 963 32 389 32 3 32
Cuts at: 1892 2266 2298 2706
Size: 374 32 408
Fragments arranged by size:
963 409 408 389 374 32 32 32
32 32 3
MfeI C'AATT_G
Cuts at: 0 1681 2706
Size: 1681 1025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 2706
Size: 809 1897
MslI CAynn'nnrTG
Cuts at: 0 2233 2706
Size: 2233 473
MspA1I CmG'CkG
Cuts at: 0 631 882 2706
Size: 631 251 1824
Fragments arranged by size:
1824 631 251
NsiI A_TGCA'T
Cuts at: 0 2325 2706
Size: 2325 381
NspI r_CATG'y
Cuts at: 0 874 2452 2706
Size: 874 1578 254
Fragments arranged by size:
1578 874 254
PciI A'CATG_T
Cuts at: 0 870 2448 2706
Size: 870 1578 258
Fragments arranged by size:
1578 870 258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1979 2011 2706
Size: 1979 32 695
Fragments arranged by size:
1979 695 32
PshAI GACnn'nnGTC
Cuts at: 0 1022 2706
Size: 1022 1684
PsiI TTA'TAA
Cuts at: 0 637 2706
Size: 637 2069
PspOMI G'GGCC_C
Cuts at: 0 904 2706
Size: 904 1802
PspXI vC'TCGA_Gb
Cuts at: 0 1537 2706
Size: 1537 1169
PstI C_TGCA'G
Cuts at: 0 573 2706
Size: 573 2133
PvuII CAG'CTG
Cuts at: 0 631 882 2706
Size: 631 251 1824
Fragments arranged by size:
1824 631 251
SfcI C'TryA_G
Cuts at: 0 299 569 899 2706
Size: 299 270 330 1807
Fragments arranged by size:
1807 330 299 270
SmlI C'TyrA_G
Cuts at: 0 128 1027 1112 1537 1553 1887 2706
Size: 128 899 85 425 16 334 819
Fragments arranged by size:
899 819 425 334 128 85 16
SspI AAT'ATT
Cuts at: 0 1386 2706
Size: 1386 1320
StyI C'CwwG_G
Cuts at: 0 247 1719 2706
Size: 247 1472 987
Fragments arranged by size:
1472 987 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2294 2706
Size: 2294 412
TatI w'GTAC_w
Cuts at: 0 442 1533 2091 2706
Size: 442 1091 558 615
Fragments arranged by size:
1091 615 558 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1308 1340 2706
Size: 1308 32 1366
Fragments arranged by size:
1366 1308 32
XbaI T'CTAG_A
Cuts at: 0 1071 2706
Size: 1071 1635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1620 2706
Size: 1011 609 1086
Fragments arranged by size:
1086 1011 609
XhoI C'TCGA_G
Cuts at: 0 1537 2706
Size: 1537 1169
XmnI GAAnn'nnTTC
Cuts at: 0 2101 2339 2706
Size: 2101 238 367
Fragments arranged by size:
2101 367 238
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApaI ApoI AvaI
AvrII BanI BanII BbsI BciVI BfrBI BglII BlpI
Bme1580I BpmI BpuEI BsaI BsaBI BsaWI BseRI BsgI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstXI
BstYI BtsI DraI DrdI EarI Eco57MI EcoNI EcoO109I
FalI FspI Hin4I MfeI MmeI MslI MspA1I NsiI
NspI PciI PpiI PshAI PsiI PspOMI PspXI PstI
PvuII SfcI SmlI SspI StyI TaqII TatI TstI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BbvCI BcgI BclI BglI BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaHI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpuMI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XmaI ZraI