Restriction map for
pFN21AE1276
(Linear) (Six-base) MAPSORT of: KIEE1276.seq Check: 1449 from: 1 to: 2256
KIEE1276.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
Acc65I G'GTAC_C
Cuts at: 0 1648 2256
Size: 1648 608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 106 734 1949 2110 2256
Size: 25 81 628 1215 161 146
Fragments arranged by size:
1215 628 161 146 81 25
AflIII A'CryG_T
Cuts at: 0 732 917 1480 2256
Size: 732 185 563 776
Fragments arranged by size:
776 732 563 185
AhdI GACnn_n'nnGTC
Cuts at: 0 1947 2256
Size: 1947 309
AleI CACnn'nnGTG
Cuts at: 0 953 2256
Size: 953 1303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1653 1685 2256
Size: 1653 32 571
Fragments arranged by size:
1653 571 32
ApoI r'AATT_y
Cuts at: 0 540 857 1002 1069 1190 2043 2256
Size: 540 317 145 67 121 853 213
Fragments arranged by size:
853 540 317 213 145 121 67
AseI AT'TA_AT
Cuts at: 0 1183 1978 2256
Size: 1183 795 278
Fragments arranged by size:
1183 795 278
BanI G'GyrC_C
Cuts at: 0 1648 2256
Size: 1648 608
BanII G_rGCy'C
Cuts at: 0 2247 2256
Size: 2247 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1937 2170 2177 2256
Size: 1937 233 7 79
Fragments arranged by size:
1937 233 79 7
BglI GCCn_nnn'nGGC
Cuts at: 0 1030 2256
Size: 1030 1226
BglII A'GATC_T
Cuts at: 0 1636 2256
Size: 1636 620
BlpI GC'TnA_GC
Cuts at: 0 995 2256
Size: 995 1261
BmrI ACTGGGnnnn_n'
Cuts at: 0 1168 1654 2256
Size: 1168 486 602
Fragments arranged by size:
1168 602 486
Bpu10I CC'TnA_GC
Cuts at: 0 1694 2256
Size: 1694 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 2256
Size: 832 1424
BsaBI GATnn'nnATC
Cuts at: 0 609 779 977 2256
Size: 609 170 198 1279
Fragments arranged by size:
1279 609 198 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1653 1683 2256
Size: 1653 30 573
Fragments arranged by size:
1653 573 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 1556 2256
Size: 448 1108 700
Fragments arranged by size:
1108 700 448
BseYI C'CCAG_C
Cuts at: 0 1730 2256
Size: 1730 526
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 2256
Size: 656 1600
BsiHKAI G_wGCw'C
Cuts at: 0 950 2247 2256
Size: 950 1297 9
Fragments arranged by size:
1297 950 9
BsmI GAATG_Cn'
Cuts at: 0 1222 1686 2256
Size: 1222 464 570
Fragments arranged by size:
1222 570 464
Bsp1286I G_dGCh'C
Cuts at: 0 950 2247 2256
Size: 950 1297 9
Fragments arranged by size:
1297 950 9
BspHI T'CATG_A
Cuts at: 0 54 2256
Size: 54 2202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1827 2256
Size: 1827 429
BstAPI GCAn_nnn'nTGC
Cuts at: 0 490 2256
Size: 490 1766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 326 2256
Size: 326 1930
BstYI r'GATC_y
Cuts at: 0 686 780 1636 2256
Size: 686 94 856 620
Fragments arranged by size:
856 686 620 94
Bsu36I CC'TnA_GG
Cuts at: 0 404 2256
Size: 404 1852
BtgI C'CryG_G
Cuts at: 0 1025 1211 2256
Size: 1025 186 1045
Fragments arranged by size:
1045 1025 186
BtsI GCAGTG_nn'
Cuts at: 0 24 376 2256
Size: 24 352 1880
Fragments arranged by size:
1880 352 24
ClaI AT'CG_AT
Cuts at: 0 225 2256
Size: 225 2031
DraI TTT'AAA
Cuts at: 0 242 350 1772 2256
Size: 242 108 1422 484
Fragments arranged by size:
1422 484 242 108
EaeI y'GGCC_r
Cuts at: 0 1022 2256
Size: 1022 1234
EarI CTCTTCn'nnn_
Cuts at: 0 1 425 1342 1574 2256
Size: 1 424 917 232 682
Fragments arranged by size:
917 682 424 232 1
EcoICRI GAG'CTC
Cuts at: 0 2245 2256
Size: 2245 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 106 734 1949 2110 2256
Size: 25 81 628 1215 161 146
Fragments arranged by size:
1215 628 161 146 81 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1804 2256
Size: 1804 452
EcoO109I rG'GnC_Cy
Cuts at: 0 262 2256
Size: 262 1994
EcoRI G'AATT_C
Cuts at: 0 540 857 1002 1190 2256
Size: 540 317 145 188 1066
Fragments arranged by size:
1066 540 317 188 145
EcoRV GAT'ATC
Cuts at: 0 2206 2256
Size: 2206 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 347 2194 2256
Size: 347 1847 62
Fragments arranged by size:
1847 347 62
HaeII r_GCGC'y
Cuts at: 0 1426 2256
Size: 1426 830
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 904 936 1933 1965 2256
Size: 246 32 626 32 997 32 291
Fragments arranged by size:
997 626 291 246 32 32 32
HincII GTy'rAC
Cuts at: 0 1925 2256
Size: 1925 331
KpnI G_GTAC'C
Cuts at: 0 1652 2256
Size: 1652 604
MfeI C'AATT_G
Cuts at: 0 720 2256
Size: 720 1536
MslI CAynn'nnrTG
Cuts at: 0 59 953 2256
Size: 59 894 1303
Fragments arranged by size:
1303 894 59
MspA1I CmG'CkG
Cuts at: 0 832 2256
Size: 832 1424
NcoI C'CATG_G
Cuts at: 0 1211 2256
Size: 1211 1045
NdeI CA'TA_TG
Cuts at: 0 650 2256
Size: 650 1606
NspI r_CATG'y
Cuts at: 0 736 921 1484 1947 2256
Size: 736 185 563 463 309
Fragments arranged by size:
736 563 463 309 185
PasI CC'CwG_GG
Cuts at: 0 691 2256
Size: 691 1565
PciI A'CATG_T
Cuts at: 0 732 917 1480 2256
Size: 732 185 563 776
Fragments arranged by size:
776 732 563 185
PfoI T'CCnGG_A
Cuts at: 0 421 504 2256
Size: 421 83 1752
Fragments arranged by size:
1752 421 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1653 1685 2256
Size: 1653 32 571
Fragments arranged by size:
1653 571 32
PpuMI rG'GwC_Cy
Cuts at: 0 262 2256
Size: 262 1994
PvuII CAG'CTG
Cuts at: 0 832 2256
Size: 832 1424
SacI G_AGCT'C
Cuts at: 0 2247 2256
Size: 2247 9
ScaI AGT'ACT
Cuts at: 0 2214 2235 2256
Size: 2214 21 21
Fragments arranged by size:
2214 21 21
SfcI C'TryA_G
Cuts at: 0 1106 2256
Size: 1106 1150
SmlI C'TyrA_G
Cuts at: 0 811 2256
Size: 811 1445
SpeI A'CTAG_T
Cuts at: 0 952 1878 2256
Size: 952 926 378
Fragments arranged by size:
952 926 378
StyI C'CwwG_G
Cuts at: 0 265 1211 2256
Size: 265 946 1045
Fragments arranged by size:
1045 946 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1023 2256
Size: 1023 1233
TatI w'GTAC_w
Cuts at: 0 1239 2212 2233 2256
Size: 1239 973 21 23
Fragments arranged by size:
1239 973 23 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1319 1351 2256
Size: 1319 32 905
Fragments arranged by size:
1319 905 32
XbaI T'CTAG_A
Cuts at: 0 1083 2256
Size: 1083 1173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1017 2256
Size: 1017 1239
XmnI GAAnn'nnTTC
Cuts at: 0 302 734 879 1286 1757 2256
Size: 302 432 145 407 471 499
Fragments arranged by size:
499 471 432 407 302 145
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AloI ApoI AseI
BanI BanII BbsI BglI BglII BlpI BmrI Bpu10I
BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BstAPI BstXI BstYI Bsu36I BtgI
BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII KpnI
MfeI MslI MspA1I NcoI NdeI NspI PasI PciI
PfoI PpiI PpuMI PvuII SacI ScaI SfcI SmlI
SpeI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AlfI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI Bme1580I
BmgBI BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI CspCI DraIII
DrdI EagI EciI FseI FspI FspAI HindIII HpaI
KasI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI
Tth111I XhoI XmaI ZraI