Restriction map for
pFN21AE1273
(Linear) (Six-base) MAPSORT of: KIEE1273.seq Check: 8683 from: 1 to: 2088
KIEE1273.orf
With 182 enzymes: SgfI *
June 21, 2019 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1248 1347 2088
Size: 1248 99 741
Fragments arranged by size:
1248 741 99
AccI GT'mk_AC
Cuts at: 0 524 2088
Size: 524 1564
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1169 1898 1931 2088
Size: 420 749 729 33 157
Fragments arranged by size:
749 729 420 157 33
AleI CACnn'nnGTG
Cuts at: 0 662 1166 2088
Size: 662 504 922
Fragments arranged by size:
922 662 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1184 2088
Size: 1184 904
AlwNI CAG_nnn'CTG
Cuts at: 0 48 886 1245 1278 1314 1612 2059 2088
Size: 48 838 359 33 36 298 447 29
Fragments arranged by size:
838 447 359 298 48 36 33 29
ApaLI G'TGCA_C
Cuts at: 0 335 665 2088
Size: 335 330 1423
Fragments arranged by size:
1423 335 330
ApoI r'AATT_y
Cuts at: 0 612 937 1204 1681 1713 2088
Size: 612 325 267 477 32 375
Fragments arranged by size:
612 477 375 325 267 32
AvaI C'yCGr_G
Cuts at: 0 827 1592 2088
Size: 827 765 496
Fragments arranged by size:
827 765 496
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 659 905 2048 2088
Size: 437 222 246 1143 40
Fragments arranged by size:
1143 437 246 222 40
BbvCI CC'TCA_GC
Cuts at: 0 1172 2088
Size: 1172 916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1960 1994 2088
Size: 1960 34 94
Fragments arranged by size:
1960 94 34
BclI T'GATC_A
Cuts at: 0 1199 2088
Size: 1199 889
BglI GCCn_nnn'nGGC
Cuts at: 0 2000 2088
Size: 2000 88
BlpI GC'TnA_GC
Cuts at: 0 1391 2088
Size: 1391 697
Bme1580I G_kGCm'C
Cuts at: 0 339 669 1163 1325 2088
Size: 339 330 494 162 763
Fragments arranged by size:
763 494 339 330 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 2088
Size: 171 1917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 2088
Size: 186 1902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1048 1918 2088
Size: 463 585 870 170
Fragments arranged by size:
870 585 463 170
Bpu10I CC'TnA_GC
Cuts at: 0 1172 2088
Size: 1172 916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 671 1090 1417 2088
Size: 127 544 419 327 671
Fragments arranged by size:
671 544 419 327 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 1909 2088
Size: 1909 179
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1006 1024 1045 1087 1111 1279 1414
Size: 391 615 18 21 42 24 168 135
Cuts at: 1414 1870 2035 2047 2088
Size: 456 165 12 41
Fragments arranged by size:
615 456 391 168 165 135 42 41
24 21 18 12
BseYI C'CCAG_C
Cuts at: 0 1271 1647 2088
Size: 1271 376 441
Fragments arranged by size:
1271 441 376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 856 1273 2088
Size: 662 194 417 815
Fragments arranged by size:
815 662 417 194
BsiEI CG_ry'CG
Cuts at: 0 1411 2088
Size: 1411 677
BsiHKAI G_wGCw'C
Cuts at: 0 19 339 669 1016 2088
Size: 19 320 330 347 1072
Fragments arranged by size:
1072 347 330 320 19
Bsp1286I G_dGCh'C
Cuts at: 0 19 339 669 1016 1163 1325 2088
Size: 19 320 330 347 147 162 763
Fragments arranged by size:
763 347 330 320 162 147 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1038 1248 1347 2088
Size: 1038 210 99 741
Fragments arranged by size:
1038 741 210 99
BsrFI r'CCGG_y
Cuts at: 0 1406 2088
Size: 1406 682
BssSI C'ACGA_G
Cuts at: 0 373 1577 2088
Size: 373 1204 511
Fragments arranged by size:
1204 511 373
BstAPI GCAn_nnn'nTGC
Cuts at: 0 673 1314 2088
Size: 673 641 774
Fragments arranged by size:
774 673 641
BstBI TT'CG_AA
Cuts at: 0 741 2088
Size: 741 1347
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1152 2088
Size: 1152 936
BstYI r'GATC_y
Cuts at: 0 1554 2088
Size: 1554 534
BstZ17I GTA'TAC
Cuts at: 0 525 2088
Size: 525 1563
Bsu36I CC'TnA_GG
Cuts at: 0 989 2088
Size: 989 1099
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 103 400 2088
Size: 103 297 1688
Fragments arranged by size:
1688 297 103
BtsI GCAGTG_nn'
Cuts at: 0 1256 2088
Size: 1256 832
EaeI y'GGCC_r
Cuts at: 0 1408 2088
Size: 1408 680
EagI C'GGCC_G
Cuts at: 0 1408 2088
Size: 1408 680
EarI CTCTTCn'nnn_
Cuts at: 0 401 533 885 1001 1727 1838 1847 1874
Size: 401 132 352 116 726 111 9 27
Cuts at: 1874 1907 1961 2024 2070 2088
Size: 33 54 63 46 18
Fragments arranged by size:
726 401 352 132 116 111 63 54
46 33 27 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 162 2088
Size: 162 1926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 463 1048 1169 1898 1918 1931 2088
Size: 420 43 585 121 729 20 13 157
Fragments arranged by size:
729 585 420 157 121 43 20 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1030 2088
Size: 1030 1058
EcoO109I rG'GnC_Cy
Cuts at: 0 1023 1588 2088
Size: 1023 565 500
Fragments arranged by size:
1023 565 500
EcoRV GAT'ATC
Cuts at: 0 1467 2088
Size: 1467 621
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 253 283 2088
Size: 253 30 1805
Fragments arranged by size:
1805 253 30
FseI GG_CCGG'CC
Cuts at: 0 1410 2088
Size: 1410 678
HaeII r_GCGC'y
Cuts at: 0 142 2088
Size: 142 1946
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1594 1626 2088
Size: 1594 32 462
Fragments arranged by size:
1594 462 32
HindIII A'AGCT_T
Cuts at: 0 1295 2088
Size: 1295 793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 987 1211 2088
Size: 372 615 224 877
Fragments arranged by size:
877 615 372 224
MslI CAynn'nnrTG
Cuts at: 0 662 1166 1448 1538 2088
Size: 662 504 282 90 550
Fragments arranged by size:
662 550 504 282 90
MspA1I CmG'CkG
Cuts at: 0 117 1278 1630 2088
Size: 117 1161 352 458
Fragments arranged by size:
1161 458 352 117
NaeI GCC'GGC
Cuts at: 0 1408 2088
Size: 1408 680
NgoMIV G'CCGG_C
Cuts at: 0 1406 2088
Size: 1406 682
PfoI T'CCnGG_A
Cuts at: 0 885 1151 2088
Size: 885 266 937
Fragments arranged by size:
937 885 266
PpuMI rG'GwC_Cy
Cuts at: 0 1588 2088
Size: 1588 500
PsiI TTA'TAA
Cuts at: 0 275 2088
Size: 275 1813
PstI C_TGCA'G
Cuts at: 0 110 1319 1382 2055 2088
Size: 110 1209 63 673 33
Fragments arranged by size:
1209 673 110 63 33
PvuII CAG'CTG
Cuts at: 0 117 1278 1630 2088
Size: 117 1161 352 458
Fragments arranged by size:
1161 458 352 117
SapI GCTCTTCn'nnn_
Cuts at: 0 885 1727 1961 2070 2088
Size: 885 842 234 109 18
Fragments arranged by size:
885 842 234 109 18
SfcI C'TryA_G
Cuts at: 0 106 1315 1378 1631 2051 2088
Size: 106 1209 63 253 420 37
Fragments arranged by size:
1209 420 253 106 63 37
SmlI C'TyrA_G
Cuts at: 0 142 686 1069 1396 2088
Size: 142 544 383 327 692
Fragments arranged by size:
692 544 383 327 142
StyI C'CwwG_G
Cuts at: 0 1497 2088
Size: 1497 591
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 937 2088
Size: 937 1151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 329 361 1096 1128 2088
Size: 148 32 149 32 735 32 960
Fragments arranged by size:
960 735 149 148 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 263 881 2088
Size: 263 618 1207
Fragments arranged by size:
1207 618 263
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaLI ApoI
AvaI BbsI BbvCI BcgI BclI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI
FseI HaeII Hin4I HindIII MmeI MslI MspA1I NaeI
NgoMIV PfoI PpuMI PsiI PstI PvuII SapI SfcI
SmlI StyI TaqII TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanI BanII BbeI BciVI BfrBI BglII BmgBI BmtI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstEII BtgI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI