Restriction map for
pFN21AE1254
(Linear) (Six-base) MAPSORT of: KIEE1254.seq Check: 7410 from: 1 to: 1641
KIEE1254.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AccI GT'mk_AC
Cuts at: 0 4 349 1641
Size: 4 345 1292
Fragments arranged by size:
1292 345 4
AclI AA'CG_TT
Cuts at: 0 1074 1641
Size: 1074 567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 719 985 1641
Size: 109 610 266 656
Fragments arranged by size:
656 610 266 109
AhdI GACnn_n'nnGTC
Cuts at: 0 734 1641
Size: 734 907
AleI CACnn'nnGTG
Cuts at: 0 575 721 1641
Size: 575 146 920
Fragments arranged by size:
920 575 146
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 1641
Size: 538 32 1071
Fragments arranged by size:
1071 538 32
ApoI r'AATT_y
Cuts at: 0 519 1491 1641
Size: 519 972 150
Fragments arranged by size:
972 519 150
AseI AT'TA_AT
Cuts at: 0 440 1641
Size: 440 1201
BamHI G'GATC_C
Cuts at: 0 851 1641
Size: 851 790
BanII G_rGCy'C
Cuts at: 0 873 1641
Size: 873 768
BbsI GAAGACnn'nnnn_
Cuts at: 0 119 287 401 733 1532 1641
Size: 119 168 114 332 799 109
Fragments arranged by size:
799 332 168 119 114 109
BbvCI CC'TCA_GC
Cuts at: 0 985 1641
Size: 985 656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 745 779 1641
Size: 745 34 862
Fragments arranged by size:
862 745 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 988 1096 1641
Size: 988 108 545
Fragments arranged by size:
988 545 108
BglI GCCn_nnn'nGGC
Cuts at: 0 1411 1641
Size: 1411 230
Bme1580I G_kGCm'C
Cuts at: 0 1407 1641
Size: 1407 234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1256 1577 1641
Size: 1256 321 64
Fragments arranged by size:
1256 321 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1641
Size: 859 782
Bpu10I CC'TnA_GC
Cuts at: 0 148 985 1641
Size: 148 837 656
Fragments arranged by size:
837 656 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1641
Size: 691 950
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 355 385 1248 1278 1641
Size: 355 30 863 30 363
Fragments arranged by size:
863 363 355 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 97 370 373 376 379 382 400
Size: 52 45 273 3 3 3 3 18
Cuts at: 400 1245 1253 1256 1259 1268 1271 1274 1277
Size: 845 8 3 3 9 3 3 3
Cuts at: 1277 1313 1316 1319 1328 1641
Size: 36 3 3 9 313
Fragments arranged by size:
845 313 273 52 45 36 18 9
9 8 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 255 558 990 1641
Size: 255 303 432 651
Fragments arranged by size:
651 432 303 255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1641
Size: 785 856
BsiHKAI G_wGCw'C
Cuts at: 0 806 873 893 1641
Size: 806 67 20 748
Fragments arranged by size:
806 748 67 20
Bsp1286I G_dGCh'C
Cuts at: 0 806 873 893 1407 1641
Size: 806 67 20 514 234
Fragments arranged by size:
806 514 234 67 20
BspHI T'CATG_A
Cuts at: 0 863 1563 1641
Size: 863 700 78
Fragments arranged by size:
863 700 78
BsrDI GCAATG_nn'
Cuts at: 0 543 630 776 1373 1641
Size: 543 87 146 597 268
Fragments arranged by size:
597 543 268 146 87
BstYI r'GATC_y
Cuts at: 0 851 1641
Size: 851 790
BstZ17I GTA'TAC
Cuts at: 0 350 1641
Size: 350 1291
BtgI C'CryG_G
Cuts at: 0 140 770 1641
Size: 140 630 871
Fragments arranged by size:
871 630 140
DraI TTT'AAA
Cuts at: 0 498 1641
Size: 498 1143
EaeI y'GGCC_r
Cuts at: 0 740 773 857 1253 1641
Size: 740 33 84 396 388
Fragments arranged by size:
740 396 388 84 33
EarI CTCTTCn'nnn_
Cuts at: 0 15 296 377 401 1164 1641
Size: 15 281 81 24 763 477
Fragments arranged by size:
763 477 281 81 24 15
EcoICRI GAG'CTC
Cuts at: 0 871 1641
Size: 871 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 719 859 985 1641
Size: 109 610 140 126 656
Fragments arranged by size:
656 610 140 126 109
EcoO109I rG'GnC_Cy
Cuts at: 0 1114 1641
Size: 1114 527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 322 1641
Size: 322 1319
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 720 752 1221 1253 1641
Size: 355 32 333 32 469 32 388
Fragments arranged by size:
469 388 355 333 32 32 32
MfeI C'AATT_G
Cuts at: 0 419 1641
Size: 419 1222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 854 1641
Size: 89 765 787
Fragments arranged by size:
787 765 89
MscI TGG'CCA
Cuts at: 0 742 775 859 1255 1641
Size: 742 33 84 396 386
Fragments arranged by size:
742 396 386 84 33
MslI CAynn'nnrTG
Cuts at: 0 575 721 1641
Size: 575 146 920
Fragments arranged by size:
920 575 146
MspA1I CmG'CkG
Cuts at: 0 562 1641
Size: 562 1079
NcoI C'CATG_G
Cuts at: 0 770 1641
Size: 770 871
NspI r_CATG'y
Cuts at: 0 754 1641
Size: 754 887
PflMI CCAn_nnn'nTGG
Cuts at: 0 1136 1641
Size: 1136 505
PpuMI rG'GwC_Cy
Cuts at: 0 1114 1641
Size: 1114 527
PshAI GACnn'nnGTC
Cuts at: 0 1132 1641
Size: 1132 509
PvuII CAG'CTG
Cuts at: 0 562 1641
Size: 562 1079
SacI G_AGCT'C
Cuts at: 0 873 1641
Size: 873 768
SapI GCTCTTCn'nnn_
Cuts at: 0 296 1164 1641
Size: 296 868 477
Fragments arranged by size:
868 477 296
SfcI C'TryA_G
Cuts at: 0 587 1641
Size: 587 1054
SmlI C'TyrA_G
Cuts at: 0 706 1641
Size: 706 935
SphI G_CATG'C
Cuts at: 0 754 1641
Size: 754 887
StyI C'CwwG_G
Cuts at: 0 770 1641
Size: 770 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1248 1280 1641
Size: 1248 32 361
Fragments arranged by size:
1248 361 32
XmnI GAAnn'nnTTC
Cuts at: 0 730 1583 1641
Size: 730 853 58
Fragments arranged by size:
853 730 58
Enzymes that do cut:
AccI AclI AcuI AhdI AleI AloI ApoI AseI
BamHI BanII BbsI BbvCI BcgI BciVI BglI Bme1580I
BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrDI BstYI BstZ17I BtgI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MfeI
MmeI MscI MslI MspA1I NcoI NspI PflMI PpuMI
PshAI PvuII SacI SapI SfcI SmlI SphI StyI
TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BanI BbeI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI ZraI