Restriction map for
pFN21AE1250
(Linear) (Six-base) MAPSORT of: KIEE1250.seq Check: 6901 from: 1 to: 1518
KIEE1250.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 395 1486 1518
Size: 395 1091 32
Fragments arranged by size:
1091 395 32
AatII G_ACGT'C
Cuts at: 0 8 1518
Size: 8 1510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 753 940 1518
Size: 656 97 187 578
Fragments arranged by size:
656 578 187 97
AflIII A'CryG_T
Cuts at: 0 824 1518
Size: 824 694
AleI CACnn'nnGTG
Cuts at: 0 503 1518
Size: 503 1015
AlwNI CAG_nnn'CTG
Cuts at: 0 1215 1478 1518
Size: 1215 263 40
Fragments arranged by size:
1215 263 40
ApaI G_GGCC'C
Cuts at: 0 467 1518
Size: 467 1051
AvaI C'yCGr_G
Cuts at: 0 46 450 460 654 1389 1518
Size: 46 404 10 194 735 129
Fragments arranged by size:
735 404 194 129 46 10
BamHI G'GATC_C
Cuts at: 0 1393 1518
Size: 1393 125
BanI G'GyrC_C
Cuts at: 0 24 1240 1518
Size: 24 1216 278
Fragments arranged by size:
1216 278 24
BanII G_rGCy'C
Cuts at: 0 149 467 964 1135 1336 1518
Size: 149 318 497 171 201 182
Fragments arranged by size:
497 318 201 182 171 149
BbeI G_GCGC'C
Cuts at: 0 28 1244 1518
Size: 28 1216 274
Fragments arranged by size:
1216 274 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 898 1518
Size: 200 698 620
Fragments arranged by size:
698 620 200
BbvCI CC'TCA_GC
Cuts at: 0 511 1518
Size: 511 1007
BglI GCCn_nnn'nGGC
Cuts at: 0 58 67 940 1518
Size: 58 9 873 578
Fragments arranged by size:
873 578 58 9
BglII A'GATC_T
Cuts at: 0 650 1518
Size: 650 868
BlpI GC'TnA_GC
Cuts at: 0 1192 1518
Size: 1192 326
Bme1580I G_kGCm'C
Cuts at: 0 467 510 1518
Size: 467 43 1008
Fragments arranged by size:
1008 467 43
BmgBI CAC'GTC
Cuts at: 0 825 1518
Size: 825 693
BmrI ACTGGGnnnn_n'
Cuts at: 0 800 1446 1448 1518
Size: 800 646 2 70
Fragments arranged by size:
800 646 70 2
BmtI G_CTAG'C
Cuts at: 0 373 947 1518
Size: 373 574 571
Fragments arranged by size:
574 571 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 1297 1328 1518
Size: 594 703 31 190
Fragments arranged by size:
703 594 190 31
Bpu10I CC'TnA_GC
Cuts at: 0 511 1518
Size: 511 1007
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 973 1518
Size: 660 313 545
Fragments arranged by size:
660 545 313
BsaAI yAC'GTr
Cuts at: 0 120 1518
Size: 120 1398
BsaBI GATnn'nnATC
Cuts at: 0 541 1518
Size: 541 977
BsaHI Gr'CG_yC
Cuts at: 0 5 25 1069 1241 1518
Size: 5 20 1044 172 277
Fragments arranged by size:
1044 277 172 20 5
BsaWI w'CCGG_w
Cuts at: 0 545 1518
Size: 545 973
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 588 618 1518
Size: 588 30 900
Fragments arranged by size:
900 588 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 1237 1518
Size: 643 594 281
Fragments arranged by size:
643 594 281
BseYI C'CCAG_C
Cuts at: 0 345 471 521 1128 1272 1280 1381 1518
Size: 345 126 50 607 144 8 101 137
Fragments arranged by size:
607 345 144 137 126 101 50 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1189 1339 1518
Size: 232 957 150 179
Fragments arranged by size:
957 232 179 150
BsiEI CG_ry'CG
Cuts at: 0 62 1518
Size: 62 1456
BsiHKAI G_wGCw'C
Cuts at: 0 149 964 991 1518
Size: 149 815 27 527
Fragments arranged by size:
815 527 149 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 957 1518
Size: 957 561
Bsp1286I G_dGCh'C
Cuts at: 0 149 467 510 964 991 1135 1336 1518
Size: 149 318 43 454 27 144 201 182
Fragments arranged by size:
454 318 201 182 149 144 43 27
BspEI T'CCGG_A
Cuts at: 0 545 1518
Size: 545 973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 1088 1486 1518
Size: 395 693 398 32
Fragments arranged by size:
693 398 395 32
BsrBI CCG'CTC
Cuts at: 0 1191 1518
Size: 1191 327
BsrDI GCAATG_nn'
Cuts at: 0 491 1518
Size: 491 1027
BsrFI r'CCGG_y
Cuts at: 0 57 61 455 1518
Size: 57 4 394 1063
Fragments arranged by size:
1063 394 57 4
BssHII G'CGCG_C
Cuts at: 0 38 1518
Size: 38 1480
BstXI CCAn_nnnn'nTGG
Cuts at: 0 837 1305 1518
Size: 837 468 213
Fragments arranged by size:
837 468 213
BstYI r'GATC_y
Cuts at: 0 203 650 1393 1518
Size: 203 447 743 125
Fragments arranged by size:
743 447 203 125
BtgI C'CryG_G
Cuts at: 0 29 87 288 335 1304 1518
Size: 29 58 201 47 969 214
Fragments arranged by size:
969 214 201 58 47 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 1342 1518
Size: 540 802 176
Fragments arranged by size:
802 540 176
BtsI GCAGTG_nn'
Cuts at: 0 1101 1201 1219 1518
Size: 1101 100 18 299
Fragments arranged by size:
1101 299 100 18
EaeI y'GGCC_r
Cuts at: 0 59 174 491 1518
Size: 59 115 317 1027
Fragments arranged by size:
1027 317 115 59
EagI C'GGCC_G
Cuts at: 0 59 1518
Size: 59 1459
EarI CTCTTCn'nnn_
Cuts at: 0 620 769 1518
Size: 620 149 749
Fragments arranged by size:
749 620 149
EcoICRI GAG'CTC
Cuts at: 0 147 962 1518
Size: 147 815 556
Fragments arranged by size:
815 556 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 656 753 940 1297 1328 1518
Size: 594 62 97 187 357 31 190
Fragments arranged by size:
594 357 190 187 97 62 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 862 885 1518
Size: 862 23 633
Fragments arranged by size:
862 633 23
EcoO109I rG'GnC_Cy
Cuts at: 0 464 723 924 1100 1518
Size: 464 259 201 176 418
Fragments arranged by size:
464 418 259 201 176
FseI GG_CCGG'CC
Cuts at: 0 65 459 1518
Size: 65 394 1059
Fragments arranged by size:
1059 394 65
HaeII r_GCGC'y
Cuts at: 0 28 1244 1518
Size: 28 1216 274
Fragments arranged by size:
1216 274 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 204 236 588 620 1518
Size: 136 32 36 32 352 32 898
Fragments arranged by size:
898 352 136 36 32 32 32
HincII GTy'rAC
Cuts at: 0 155 1518
Size: 155 1363
KasI G'GCGC_C
Cuts at: 0 24 1240 1518
Size: 24 1216 278
Fragments arranged by size:
1216 278 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 614 1055 1439 1518
Size: 386 228 441 384 79
Fragments arranged by size:
441 386 384 228 79
MscI TGG'CCA
Cuts at: 0 493 1518
Size: 493 1025
MslI CAynn'nnrTG
Cuts at: 0 355 503 1303 1518
Size: 355 148 800 215
Fragments arranged by size:
800 355 215 148
MspA1I CmG'CkG
Cuts at: 0 31 402 1215 1323 1430 1518
Size: 31 371 813 108 107 88
Fragments arranged by size:
813 371 108 107 88 31
NaeI GCC'GGC
Cuts at: 0 59 63 457 1518
Size: 59 4 394 1061
Fragments arranged by size:
1061 394 59 4
NarI GG'CG_CC
Cuts at: 0 25 1241 1518
Size: 25 1216 277
Fragments arranged by size:
1216 277 25
NcoI C'CATG_G
Cuts at: 0 335 1304 1518
Size: 335 969 214
Fragments arranged by size:
969 335 214
NgoMIV G'CCGG_C
Cuts at: 0 57 61 455 1518
Size: 57 4 394 1063
Fragments arranged by size:
1063 394 57 4
NheI G'CTAG_C
Cuts at: 0 369 943 1518
Size: 369 574 575
Fragments arranged by size:
575 574 369
NspI r_CATG'y
Cuts at: 0 369 1269 1518
Size: 369 900 249
Fragments arranged by size:
900 369 249
PasI CC'CwG_GG
Cuts at: 0 1236 1493 1518
Size: 1236 257 25
Fragments arranged by size:
1236 257 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1518
Size: 420 1098
PfoI T'CCnGG_A
Cuts at: 0 230 413 1518
Size: 230 183 1105
Fragments arranged by size:
1105 230 183
PpuMI rG'GwC_Cy
Cuts at: 0 723 1518
Size: 723 795
PspOMI G'GGCC_C
Cuts at: 0 463 1518
Size: 463 1055
PstI C_TGCA'G
Cuts at: 0 1085 1483 1518
Size: 1085 398 35
Fragments arranged by size:
1085 398 35
PvuII CAG'CTG
Cuts at: 0 402 1215 1323 1518
Size: 402 813 108 195
Fragments arranged by size:
813 402 195 108
SacI G_AGCT'C
Cuts at: 0 149 964 1518
Size: 149 815 554
Fragments arranged by size:
815 554 149
SacII CC_GC'GG
Cuts at: 0 32 1518
Size: 32 1486
SbfI CC_TGCA'GG
Cuts at: 0 1085 1518
Size: 1085 433
SfcI C'TryA_G
Cuts at: 0 1081 1479 1518
Size: 1081 398 39
Fragments arranged by size:
1081 398 39
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 58 1518
Size: 58 1460
SfoI GGC'GCC
Cuts at: 0 26 1242 1518
Size: 26 1216 276
Fragments arranged by size:
1216 276 26
SmaI CCC'GGG
Cuts at: 0 48 462 1518
Size: 48 414 1056
Fragments arranged by size:
1056 414 48
SrfI GCCC'GGGC
Cuts at: 0 462 1518
Size: 462 1056
StuI AGG'CCT
Cuts at: 0 880 1052 1087 1154 1518
Size: 880 172 35 67 364
Fragments arranged by size:
880 364 172 67 35
StyI C'CwwG_G
Cuts at: 0 335 1304 1518
Size: 335 969 214
Fragments arranged by size:
969 335 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1038 1518
Size: 1038 480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1169 1201 1343 1375 1518
Size: 1169 32 142 32 143
Fragments arranged by size:
1169 143 142 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1071 1518
Size: 1071 447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1050 1518
Size: 1050 468
XmaI C'CCGG_G
Cuts at: 0 46 460 1518
Size: 46 414 1058
Fragments arranged by size:
1058 414 46
ZraI GAC'GTC
Cuts at: 0 6 1518
Size: 6 1512
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstXI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SbfI SfcI
SfiI SfoI SmaI SrfI StuI StyI TaqII TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BplI BpuEI BsiWI BsmI
BspHI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV
FalI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI ScaI SexAI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI SwaI TatI XbaI XhoI XmnI