Restriction map for
pFN21AE1247
(Linear) (Six-base) MAPSORT of: KIEE1247.seq Check: 241 from: 1 to: 1446
KIEE1247.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1402 1446
Size: 1402 44
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1358 1446
Size: 128 1230 88
Fragments arranged by size:
1230 128 88
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 1446
Size: 82 32 1332
Fragments arranged by size:
1332 82 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1014 1041 1446
Size: 1014 27 405
Fragments arranged by size:
1014 405 27
ApaI G_GGCC'C
Cuts at: 0 371 1446
Size: 371 1075
BamHI G'GATC_C
Cuts at: 0 97 1446
Size: 97 1349
BanI G'GyrC_C
Cuts at: 0 34 1446
Size: 34 1412
BanII G_rGCy'C
Cuts at: 0 17 371 481 1346 1446
Size: 17 354 110 865 100
Fragments arranged by size:
865 354 110 100 17
BbeI G_GCGC'C
Cuts at: 0 38 1446
Size: 38 1408
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 1446
Size: 173 1273
BciVI GTATCCnnnnn_n'
Cuts at: 0 344 1446
Size: 344 1102
BglI GCCn_nnn'nGGC
Cuts at: 0 43 1446
Size: 43 1403
Bme1580I G_kGCm'C
Cuts at: 0 371 947 1446
Size: 371 576 499
Fragments arranged by size:
576 499 371
BmgBI CAC'GTC
Cuts at: 0 798 1446
Size: 798 648
BmrI ACTGGGnnnn_n'
Cuts at: 0 972 1446
Size: 972 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 709 799 937 1036 1195 1261 1273
Size: 202 507 90 138 99 159 66 12
Cuts at: 1273 1446
Size: 173
Fragments arranged by size:
507 202 173 159 138 99 90 66
12
Bpu10I CC'TnA_GC
Cuts at: 0 499 1321 1446
Size: 499 822 125
Fragments arranged by size:
822 499 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 1446
Size: 1418 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 1119 1446
Size: 1119 327
BsaBI GATnn'nnATC
Cuts at: 0 881 1446
Size: 881 565
BsaHI Gr'CG_yC
Cuts at: 0 35 1446
Size: 35 1411
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 304 334 410 440 1009 1039
Size: 232 30 42 30 76 30 569 30
Cuts at: 1039 1414 1444 1446
Size: 375 30 2
Fragments arranged by size:
569 375 232 76 42 30 30 30
30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 68 253 271 307 325 469 505 586
Size: 68 185 18 36 18 144 36 81
Cuts at: 586 724 1033 1036 1446
Size: 138 309 3 410
Fragments arranged by size:
410 309 185 144 138 81 68 36
36 18 18 3
BseYI C'CCAG_C
Cuts at: 0 481 560 845 866 1066 1446
Size: 481 79 285 21 200 380
Fragments arranged by size:
481 380 285 200 79 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1240 1446
Size: 1240 206
BsiHKAI G_wGCw'C
Cuts at: 0 404 1346 1446
Size: 404 942 100
Fragments arranged by size:
942 404 100
Bsp1286I G_dGCh'C
Cuts at: 0 17 371 404 481 947 1346 1446
Size: 17 354 33 77 466 399 100
Fragments arranged by size:
466 399 354 100 77 33 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1402 1446
Size: 1402 44
BsrFI r'CCGG_y
Cuts at: 0 962 1446
Size: 962 484
BstEII G'GTnAC_C
Cuts at: 0 193 1446
Size: 193 1253
BstXI CCAn_nnnn'nTGG
Cuts at: 0 940 1380 1446
Size: 940 440 66
Fragments arranged by size:
940 440 66
BstYI r'GATC_y
Cuts at: 0 97 713 1100 1446
Size: 97 616 387 346
Fragments arranged by size:
616 387 346 97
Bsu36I CC'TnA_GG
Cuts at: 0 452 703 1446
Size: 452 251 743
Fragments arranged by size:
743 452 251
BtgI C'CryG_G
Cuts at: 0 335 371 1446
Size: 335 36 1075
Fragments arranged by size:
1075 335 36
BtsI GCAGTG_nn'
Cuts at: 0 547 1446
Size: 547 899
EaeI y'GGCC_r
Cuts at: 0 2 1446
Size: 2 1444
EarI CTCTTCn'nnn_
Cuts at: 0 86 563 1208 1334 1446
Size: 86 477 645 126 112
Fragments arranged by size:
645 477 126 112 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 1144 1446
Size: 451 693 302
Fragments arranged by size:
693 451 302
EcoICRI GAG'CTC
Cuts at: 0 1344 1446
Size: 1344 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 202 709 799 937 1036 1195 1261
Size: 128 74 507 90 138 99 159 66
Cuts at: 1261 1273 1358 1446
Size: 12 85 88
Fragments arranged by size:
507 159 138 128 99 90 88 85
74 66 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 106 625 706 1018 1384 1446
Size: 106 519 81 312 366 62
Fragments arranged by size:
519 366 312 106 81 62
EcoO109I rG'GnC_Cy
Cuts at: 0 152 243 367 510 1011 1446
Size: 152 91 124 143 501 435
Fragments arranged by size:
501 435 152 143 124 91
HaeII r_GCGC'y
Cuts at: 0 38 1446
Size: 38 1408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 1062 1094 1414 1416 1446
Size: 410 32 620 32 320 2 30
Fragments arranged by size:
620 410 320 32 32 30 2
HindIII A'AGCT_T
Cuts at: 0 733 1446
Size: 733 713
KasI G'GCGC_C
Cuts at: 0 34 1446
Size: 34 1412
MscI TGG'CCA
Cuts at: 0 4 1446
Size: 4 1442
MspA1I CmG'CkG
Cuts at: 0 564 777 903 1239 1446
Size: 564 213 126 336 207
Fragments arranged by size:
564 336 213 207 126
NaeI GCC'GGC
Cuts at: 0 964 1446
Size: 964 482
NarI GG'CG_CC
Cuts at: 0 35 1446
Size: 35 1411
NgoMIV G'CCGG_C
Cuts at: 0 962 1446
Size: 962 484
NspI r_CATG'y
Cuts at: 0 289 1446
Size: 289 1157
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 1014 1446
Size: 555 459 432
Fragments arranged by size:
555 459 432
PfoI T'CCnGG_A
Cuts at: 0 608 818 872 953 1103 1446
Size: 608 210 54 81 150 343
Fragments arranged by size:
608 343 210 150 81 54
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1446
Size: 1021 32 393
Fragments arranged by size:
1021 393 32
PpuMI rG'GwC_Cy
Cuts at: 0 152 510 1011 1446
Size: 152 358 501 435
Fragments arranged by size:
501 435 358 152
PsiI TTA'TAA
Cuts at: 0 1355 1446
Size: 1355 91
PspOMI G'GGCC_C
Cuts at: 0 367 1446
Size: 367 1079
PstI C_TGCA'G
Cuts at: 0 626 1085 1446
Size: 626 459 361
Fragments arranged by size:
626 459 361
PvuII CAG'CTG
Cuts at: 0 564 777 903 1239 1446
Size: 564 213 126 336 207
Fragments arranged by size:
564 336 213 207 126
SacI G_AGCT'C
Cuts at: 0 1346 1446
Size: 1346 100
SapI GCTCTTCn'nnn_
Cuts at: 0 1334 1446
Size: 1334 112
SbfI CC_TGCA'GG
Cuts at: 0 626 1446
Size: 626 820
SfcI C'TryA_G
Cuts at: 0 622 1081 1446
Size: 622 459 365
Fragments arranged by size:
622 459 365
SfoI GGC'GCC
Cuts at: 0 36 1446
Size: 36 1410
SmlI C'TyrA_G
Cuts at: 0 1433 1446
Size: 1433 13
TatI w'GTAC_w
Cuts at: 0 1226 1446
Size: 1226 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 1446
Size: 304 32 1110
Fragments arranged by size:
1110 304 32
Tth111I GACn'n_nGTC
Cuts at: 0 1428 1446
Size: 1428 18
XmnI GAAnn'nnTTC
Cuts at: 0 170 736 1446
Size: 170 566 710
Fragments arranged by size:
710 566 170
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaI BamHI BanI BanII
BbeI BbsI BciVI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BstEII BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII KasI MscI MspA1I
NaeI NarI NgoMIV NspI PflMI PfoI PpiI PpuMI
PsiI PspOMI PstI PvuII SacI SapI SbfI SfcI
SfoI SmlI TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbvCI BcgI BclI BfrBI BglII
BlpI BmtI BplI BsaAI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PspXI PsrI PvuI RsrII SacII
SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII XbaI XcmI XhoI XmaI ZraI