Restriction map for
pFN21AE1230
(Linear) (Six-base) MAPSORT of: KIEE1230.seq Check: 3566 from: 1 to: 1029
KIEE1230.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 546 819 1029
Size: 546 273 210
Fragments arranged by size:
546 273 210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 1029
Size: 303 726
AgeI A'CCGG_T
Cuts at: 0 123 1029
Size: 123 906
AlwNI CAG_nnn'CTG
Cuts at: 0 678 1029
Size: 678 351
AvaI C'yCGr_G
Cuts at: 0 420 1029
Size: 420 609
BamHI G'GATC_C
Cuts at: 0 362 1029
Size: 362 667
BanI G'GyrC_C
Cuts at: 0 577 1029
Size: 577 452
BanII G_rGCy'C
Cuts at: 0 696 808 1029
Size: 696 112 221
Fragments arranged by size:
696 221 112
BglI GCCn_nnn'nGGC
Cuts at: 0 575 1029
Size: 575 454
Bme1580I G_kGCm'C
Cuts at: 0 580 1029
Size: 580 449
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 1029
Size: 172 857
BmtI G_CTAG'C
Cuts at: 0 97 145 1029
Size: 97 48 884
Fragments arranged by size:
884 97 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 1029
Size: 478 551
Bpu10I CC'TnA_GC
Cuts at: 0 178 1029
Size: 178 851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1029
Size: 1006 23
BsaAI yAC'GTr
Cuts at: 0 519 1029
Size: 519 510
BsaWI w'CCGG_w
Cuts at: 0 123 1029
Size: 123 906
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 1029
Size: 439 30 560
Fragments arranged by size:
560 439 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 541 1029
Size: 415 126 488
Fragments arranged by size:
488 415 126
BseYI C'CCAG_C
Cuts at: 0 169 1029
Size: 169 860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 325 493 1029
Size: 148 177 168 536
Fragments arranged by size:
536 177 168 148
BsiEI CG_ry'CG
Cuts at: 0 574 657 1029
Size: 574 83 372
Fragments arranged by size:
574 372 83
BsiHKAI G_wGCw'C
Cuts at: 0 696 1029
Size: 696 333
Bsp1286I G_dGCh'C
Cuts at: 0 580 696 808 1029
Size: 580 116 112 221
Fragments arranged by size:
580 221 116 112
BspHI T'CATG_A
Cuts at: 0 854 1029
Size: 854 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 546 819 1029
Size: 546 273 210
Fragments arranged by size:
546 273 210
BsrFI r'CCGG_y
Cuts at: 0 123 333 442 569 656 1029
Size: 123 210 109 127 87 373
Fragments arranged by size:
373 210 127 123 109 87
BstEII G'GTnAC_C
Cuts at: 0 720 1029
Size: 720 309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 635 915 1029
Size: 635 280 114
Fragments arranged by size:
635 280 114
BstYI r'GATC_y
Cuts at: 0 362 488 875 1029
Size: 362 126 387 154
Fragments arranged by size:
387 362 154 126
BtgI C'CryG_G
Cuts at: 0 32 350 768 1029
Size: 32 318 418 261
Fragments arranged by size:
418 318 261 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 806 1029
Size: 806 223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 94 129 1029
Size: 94 35 900
Fragments arranged by size:
900 94 35
DraIII CAC_nnn'GTG
Cuts at: 0 839 1029
Size: 839 190
EaeI y'GGCC_r
Cuts at: 0 254 353 571 650 917 1029
Size: 254 99 218 79 267 112
Fragments arranged by size:
267 254 218 112 99 79
EagI C'GGCC_G
Cuts at: 0 571 1029
Size: 571 458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 469 914 1029
Size: 469 445 115
Fragments arranged by size:
469 445 115
EcoICRI GAG'CTC
Cuts at: 0 694 1029
Size: 694 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 478 1029
Size: 303 175 551
Fragments arranged by size:
551 303 175
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 670 1029
Size: 481 189 359
Fragments arranged by size:
481 359 189
EcoO109I rG'GnC_Cy
Cuts at: 0 166 549 675 1029
Size: 166 383 126 354
Fragments arranged by size:
383 354 166 126
HaeII r_GCGC'y
Cuts at: 0 350 1029
Size: 350 679
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 893 925 1029
Size: 893 32 104
Fragments arranged by size:
893 104 32
MscI TGG'CCA
Cuts at: 0 256 919 1029
Size: 256 663 110
Fragments arranged by size:
663 256 110
MslI CAynn'nnrTG
Cuts at: 0 715 773 853 1029
Size: 715 58 80 176
Fragments arranged by size:
715 176 80 58
MspA1I CmG'CkG
Cuts at: 0 90 1029
Size: 90 939
NaeI GCC'GGC
Cuts at: 0 571 1029
Size: 571 458
NcoI C'CATG_G
Cuts at: 0 32 1029
Size: 32 997
NgoMIV G'CCGG_C
Cuts at: 0 569 1029
Size: 569 460
NheI G'CTAG_C
Cuts at: 0 93 141 1029
Size: 93 48 888
Fragments arranged by size:
888 93 48
PfoI T'CCnGG_A
Cuts at: 0 457 494 1029
Size: 457 37 535
Fragments arranged by size:
535 457 37
PmlI CAC'GTG
Cuts at: 0 519 1029
Size: 519 510
PpuMI rG'GwC_Cy
Cuts at: 0 166 1029
Size: 166 863
PstI C_TGCA'G
Cuts at: 0 557 1029
Size: 557 472
SacI G_AGCT'C
Cuts at: 0 696 1029
Size: 696 333
SanDI GG'GwC_CC
Cuts at: 0 166 1029
Size: 166 863
SbfI CC_TGCA'GG
Cuts at: 0 557 1029
Size: 557 472
SfcI C'TryA_G
Cuts at: 0 553 667 699 1029
Size: 553 114 32 330
Fragments arranged by size:
553 330 114 32
SmaI CCC'GGG
Cuts at: 0 422 1029
Size: 422 607
SmlI C'TyrA_G
Cuts at: 0 1021 1029
Size: 1021 8
StyI C'CwwG_G
Cuts at: 0 32 1029
Size: 32 997
Tth111I GACn'n_nGTC
Cuts at: 0 613 1029
Size: 613 416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 1029
Size: 732 297
XmaI C'CCGG_G
Cuts at: 0 420 1029
Size: 420 609
Enzymes that do cut:
AarI AcuI AgeI AlwNI AvaI BamHI BanI BanII
BglI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrFI BstEII BstXI BstYI BtgI BtgZI
CspCI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MscI MslI MspA1I NaeI NcoI
NgoMIV NheI PfoI PmlI PpuMI PstI SacI SanDI
SbfI SfcI SmaI SmlI StyI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BplI BsaI BsaBI
BsaHI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI
DraI DrdI EarI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
XbaI XhoI XmnI ZraI