Restriction map for
pFN21AE1228
(Linear) (Six-base) MAPSORT of: KIEE1228.seq Check: 6220 from: 1 to: 951
KIEE1228.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
Acc65I G'GTAC_C
Cuts at: 0 168 951
Size: 168 783
AflII C'TTAA_G
Cuts at: 0 85 951
Size: 85 866
AlwNI CAG_nnn'CTG
Cuts at: 0 580 951
Size: 580 371
ApaLI G'TGCA_C
Cuts at: 0 304 951
Size: 304 647
ApoI r'AATT_y
Cuts at: 0 391 510 518 760 951
Size: 391 119 8 242 191
Fragments arranged by size:
391 242 191 119 8
BanI G'GyrC_C
Cuts at: 0 168 951
Size: 168 783
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 340 697 951
Size: 35 305 357 254
Fragments arranged by size:
357 305 254 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 321 355 951
Size: 321 34 596
Fragments arranged by size:
596 321 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 121 316 951
Size: 121 195 635
Fragments arranged by size:
635 195 121
BglI GCCn_nnn'nGGC
Cuts at: 0 812 951
Size: 812 139
BglII A'GATC_T
Cuts at: 0 612 951
Size: 612 339
Bme1580I G_kGCm'C
Cuts at: 0 308 951
Size: 308 643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 951
Size: 544 407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 951
Size: 659 292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 875 905 951
Size: 875 30 46
Fragments arranged by size:
875 46 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 616 951
Size: 613 3 335
Fragments arranged by size:
613 335 3
BseYI C'CCAG_C
Cuts at: 0 471 939 951
Size: 471 468 12
Fragments arranged by size:
471 468 12
BsiHKAI G_wGCw'C
Cuts at: 0 197 308 951
Size: 197 111 643
Fragments arranged by size:
643 197 111
Bsp1286I G_dGCh'C
Cuts at: 0 197 308 951
Size: 197 111 643
Fragments arranged by size:
643 197 111
BsrDI GCAATG_nn'
Cuts at: 0 216 951
Size: 216 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 522 951
Size: 522 429
BstYI r'GATC_y
Cuts at: 0 612 951
Size: 612 339
DraI TTT'AAA
Cuts at: 0 60 315 951
Size: 60 255 636
Fragments arranged by size:
636 255 60
EaeI y'GGCC_r
Cuts at: 0 160 951
Size: 160 791
EarI CTCTTCn'nnn_
Cuts at: 0 401 951
Size: 401 550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 951
Size: 544 407
EcoNI CCTnn'n_nnAGG
Cuts at: 0 848 951
Size: 848 103
EcoO109I rG'GnC_Cy
Cuts at: 0 468 951
Size: 468 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 875 878 907 910 951
Size: 875 3 29 3 41
Fragments arranged by size:
875 41 29 3 3
HindIII A'AGCT_T
Cuts at: 0 53 951
Size: 53 898
KpnI G_GTAC'C
Cuts at: 0 172 951
Size: 172 779
NspI r_CATG'y
Cuts at: 0 939 951
Size: 939 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 551 951
Size: 551 400
PpuMI rG'GwC_Cy
Cuts at: 0 468 951
Size: 468 483
SanDI GG'GwC_CC
Cuts at: 0 468 951
Size: 468 483
SfcI C'TryA_G
Cuts at: 0 204 951
Size: 204 747
SmlI C'TyrA_G
Cuts at: 0 85 674 951
Size: 85 589 277
Fragments arranged by size:
589 277 85
SphI G_CATG'C
Cuts at: 0 939 951
Size: 939 12
SspI AAT'ATT
Cuts at: 0 266 951
Size: 266 685
StyI C'CwwG_G
Cuts at: 0 879 951
Size: 879 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 654 915 951
Size: 654 261 36
Fragments arranged by size:
654 261 36
XmnI GAAnn'nnTTC
Cuts at: 0 614 725 951
Size: 614 111 226
Fragments arranged by size:
614 226 111
Enzymes that do cut:
Acc65I AflII AlwNI ApaLI ApoI BanI BbsI BcgI
BciVI BglI BglII Bme1580I BpmI BpuEI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BsrDI BstXI BstYI DraI EaeI
EarI Eco57MI EcoNI EcoO109I Hin4I HindIII KpnI NspI
PflMI PpuMI SanDI SfcI SmlI SphI SspI StyI
TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BclI
BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI