Restriction map for
pFN21AE1226
(Linear) (Six-base) MAPSORT of: KIEE1226.seq Check: 7391 from: 1 to: 900
KIEE1226.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 68 900
Size: 68 832
AccI GT'mk_AC
Cuts at: 0 668 900
Size: 668 232
Acc65I G'GTAC_C
Cuts at: 0 274 900
Size: 274 626
AflIII A'CryG_T
Cuts at: 0 199 900
Size: 199 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 523 900
Size: 523 377
AlwNI CAG_nnn'CTG
Cuts at: 0 889 900
Size: 889 11
ApaLI G'TGCA_C
Cuts at: 0 256 900
Size: 256 644
AvaI C'yCGr_G
Cuts at: 0 65 89 900
Size: 65 24 811
Fragments arranged by size:
811 65 24
BanI G'GyrC_C
Cuts at: 0 145 274 286 499 900
Size: 145 129 12 213 401
Fragments arranged by size:
401 213 145 129 12
BanII G_rGCy'C
Cuts at: 0 479 535 664 836 900
Size: 479 56 129 172 64
Fragments arranged by size:
479 172 129 64 56
Bme1580I G_kGCm'C
Cuts at: 0 89 260 900
Size: 89 171 640
Fragments arranged by size:
640 171 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 362 900
Size: 362 538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 855 900
Size: 775 80 45
Fragments arranged by size:
775 80 45
Bpu10I CC'TnA_GC
Cuts at: 0 473 794 900
Size: 473 321 106
Fragments arranged by size:
473 321 106
BsaAI yAC'GTr
Cuts at: 0 681 735 900
Size: 681 54 165
Fragments arranged by size:
681 165 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 721 734 764 900
Size: 691 30 13 30 136
Fragments arranged by size:
691 136 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 900
Size: 784 116
BseYI C'CCAG_C
Cuts at: 0 77 455 900
Size: 77 378 445
Fragments arranged by size:
445 378 77
BsiHKAI G_wGCw'C
Cuts at: 0 260 479 592 836 900
Size: 260 219 113 244 64
Fragments arranged by size:
260 244 219 113 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 886 900
Size: 886 14
Bsp1286I G_dGCh'C
Cuts at: 0 89 260 479 535 592 664 836 900
Size: 89 171 219 56 57 72 172 64
Fragments arranged by size:
219 172 171 89 72 64 57 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 900
Size: 68 832
BsrBI CCG'CTC
Cuts at: 0 464 900
Size: 464 436
BsrDI GCAATG_nn'
Cuts at: 0 182 900
Size: 182 718
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 900
Size: 156 744
BstYI r'GATC_y
Cuts at: 0 119 426 538 900
Size: 119 307 112 362
Fragments arranged by size:
362 307 119 112
BtgI C'CryG_G
Cuts at: 0 123 141 567 623 900
Size: 123 18 426 56 277
Fragments arranged by size:
426 277 123 56 18
BtsI GCAGTG_nn'
Cuts at: 0 440 720 803 900
Size: 440 280 83 97
Fragments arranged by size:
440 280 97 83
DraIII CAC_nnn'GTG
Cuts at: 0 715 900
Size: 715 185
EaeI y'GGCC_r
Cuts at: 0 374 437 900
Size: 374 63 463
Fragments arranged by size:
463 374 63
EarI CTCTTCn'nnn_
Cuts at: 0 484 900
Size: 484 416
EcoICRI GAG'CTC
Cuts at: 0 477 834 900
Size: 477 357 66
Fragments arranged by size:
477 357 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 855 900
Size: 775 80 45
Fragments arranged by size:
775 80 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 342 374 413 445 508 540 691 723
Size: 342 32 39 32 63 32 151 32
Cuts at: 723 900
Size: 177
Fragments arranged by size:
342 177 151 63 39 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 278 900
Size: 278 622
MluI A'CGCG_T
Cuts at: 0 199 900
Size: 199 701
MscI TGG'CCA
Cuts at: 0 376 439 900
Size: 376 63 461
Fragments arranged by size:
461 376 63
MslI CAynn'nnrTG
Cuts at: 0 253 644 900
Size: 253 391 256
Fragments arranged by size:
391 256 253
MspA1I CmG'CkG
Cuts at: 0 193 327 363 900
Size: 193 134 36 537
Fragments arranged by size:
537 193 134 36
NcoI C'CATG_G
Cuts at: 0 123 900
Size: 123 777
NspI r_CATG'y
Cuts at: 0 706 900
Size: 706 194
PasI CC'CwG_GG
Cuts at: 0 42 900
Size: 42 858
PfoI T'CCnGG_A
Cuts at: 0 752 871 900
Size: 752 119 29
Fragments arranged by size:
752 119 29
PmlI CAC'GTG
Cuts at: 0 681 900
Size: 681 219
PstI C_TGCA'G
Cuts at: 0 11 119 192 512 822 900
Size: 11 108 73 320 310 78
Fragments arranged by size:
320 310 108 78 73 11
PvuII CAG'CTG
Cuts at: 0 193 363 900
Size: 193 170 537
Fragments arranged by size:
537 193 170
SacI G_AGCT'C
Cuts at: 0 479 836 900
Size: 479 357 64
Fragments arranged by size:
479 357 64
SapI GCTCTTCn'nnn_
Cuts at: 0 484 900
Size: 484 416
SbfI CC_TGCA'GG
Cuts at: 0 512 900
Size: 512 388
SexAI A'CCwGG_T
Cuts at: 0 307 900
Size: 307 593
SfcI C'TryA_G
Cuts at: 0 7 115 188 489 508 665 818 900
Size: 7 108 73 301 19 157 153 82
Fragments arranged by size:
301 157 153 108 82 73 19 7
SmaI CCC'GGG
Cuts at: 0 91 900
Size: 91 809
StyI C'CwwG_G
Cuts at: 0 123 900
Size: 123 777
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 607 652 900
Size: 55 552 45 248
Fragments arranged by size:
552 248 55 45
XmaI C'CCGG_G
Cuts at: 0 89 900
Size: 89 811
Enzymes that do cut:
AarI AccI Acc65I AflIII AlfI AlwNI ApaLI AvaI
BanI BanII Bme1580I BmrI BpmI Bpu10I BsaAI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI
Eco57MI Hin4I KpnI MluI MscI MslI MspA1I NcoI
NspI PasI PfoI PmlI PstI PvuII SacI SapI
SbfI SexAI SfcI SmaI StyI TaqII XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI MfeI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI