Restriction map for
pFN21AE1222
(Linear) (Six-base) MAPSORT of: KIEE1222.seq Check: 4487 from: 1 to: 735
KIEE1222.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
Acc65I G'GTAC_C
Cuts at: 0 632 735
Size: 632 103
AhdI GACnn_n'nnGTC
Cuts at: 0 572 735
Size: 572 163
AlwNI CAG_nnn'CTG
Cuts at: 0 46 710 735
Size: 46 664 25
Fragments arranged by size:
664 46 25
ApaI G_GGCC'C
Cuts at: 0 286 735
Size: 286 449
BamHI G'GATC_C
Cuts at: 0 109 735
Size: 109 626
BanI G'GyrC_C
Cuts at: 0 632 735
Size: 632 103
BanII G_rGCy'C
Cuts at: 0 234 286 320 372 388 647 735
Size: 234 52 34 52 16 259 88
Fragments arranged by size:
259 234 88 52 52 34 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 735
Size: 405 330
BciVI GTATCCnnnnn_n'
Cuts at: 0 103 250 735
Size: 103 147 485
Fragments arranged by size:
485 147 103
BglI GCCn_nnn'nGGC
Cuts at: 0 125 170 735
Size: 125 45 565
Fragments arranged by size:
565 125 45
Bme1580I G_kGCm'C
Cuts at: 0 13 286 735
Size: 13 273 449
Fragments arranged by size:
449 273 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 302 735
Size: 302 433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 209 248 272 402 605 735
Size: 152 57 39 24 130 203 130
Fragments arranged by size:
203 152 130 130 57 39 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 149 735
Size: 149 586
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 129 159 395 425 735
Size: 129 30 236 30 310
Fragments arranged by size:
310 236 129 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 735
Size: 328 407
BseYI C'CCAG_C
Cuts at: 0 577 735
Size: 577 158
BsiEI CG_ry'CG
Cuts at: 0 480 735
Size: 480 255
BsiHKAI G_wGCw'C
Cuts at: 0 320 735
Size: 320 415
Bsp1286I G_dGCh'C
Cuts at: 0 13 234 286 320 372 388 647 735
Size: 13 221 52 34 52 16 259 88
Fragments arranged by size:
259 221 88 52 52 34 16 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 640 735
Size: 640 95
BsrDI GCAATG_nn'
Cuts at: 0 429 482 518 735
Size: 429 53 36 217
Fragments arranged by size:
429 217 53 36
BsrGI T'GTAC_A
Cuts at: 0 680 735
Size: 680 55
BstYI r'GATC_y
Cuts at: 0 109 735
Size: 109 626
BtgI C'CryG_G
Cuts at: 0 114 176 735
Size: 114 62 559
Fragments arranged by size:
559 114 62
BtsI GCAGTG_nn'
Cuts at: 0 673 700 735
Size: 673 27 35
Fragments arranged by size:
673 35 27
EaeI y'GGCC_r
Cuts at: 0 477 735
Size: 477 258
EagI C'GGCC_G
Cuts at: 0 477 735
Size: 477 258
EcoICRI GAG'CTC
Cuts at: 0 318 735
Size: 318 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 209 248 272 402 605 735
Size: 152 57 39 24 130 203 130
Fragments arranged by size:
203 152 130 130 57 39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 735
Size: 568 167
EcoO109I rG'GnC_Cy
Cuts at: 0 172 282 283 574 608 735
Size: 172 110 1 291 34 127
Fragments arranged by size:
291 172 127 110 34 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 335 367 395 427 558 590
Size: 131 32 172 32 28 32 131 32
Cuts at: 590 716 735
Size: 126 19
Fragments arranged by size:
172 131 131 126 32 32 32 32
28 19
HindIII A'AGCT_T
Cuts at: 0 525 735
Size: 525 210
KpnI G_GTAC'C
Cuts at: 0 636 735
Size: 636 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 735
Size: 60 675
MslI CAynn'nnrTG
Cuts at: 0 34 308 735
Size: 34 274 427
Fragments arranged by size:
427 274 34
NcoI C'CATG_G
Cuts at: 0 176 735
Size: 176 559
NdeI CA'TA_TG
Cuts at: 0 549 735
Size: 549 186
NotI GC'GGCC_GC
Cuts at: 0 477 735
Size: 477 258
NspI r_CATG'y
Cuts at: 0 464 735
Size: 464 271
PfoI T'CCnGG_A
Cuts at: 0 105 207 735
Size: 105 102 528
Fragments arranged by size:
528 105 102
PpuMI rG'GwC_Cy
Cuts at: 0 574 608 735
Size: 574 34 127
Fragments arranged by size:
574 127 34
PspOMI G'GGCC_C
Cuts at: 0 282 735
Size: 282 453
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 700 732 735
Size: 700 32 3
Fragments arranged by size:
700 32 3
PstI C_TGCA'G
Cuts at: 0 359 383 735
Size: 359 24 352
Fragments arranged by size:
359 352 24
SacI G_AGCT'C
Cuts at: 0 320 735
Size: 320 415
SanDI GG'GwC_CC
Cuts at: 0 574 608 735
Size: 574 34 127
Fragments arranged by size:
574 127 34
SfcI C'TryA_G
Cuts at: 0 355 379 735
Size: 355 24 356
Fragments arranged by size:
356 355 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 170 735
Size: 170 565
StuI AGG'CCT
Cuts at: 0 563 735
Size: 563 172
StyI C'CwwG_G
Cuts at: 0 176 493 735
Size: 176 317 242
Fragments arranged by size:
317 242 176
TatI w'GTAC_w
Cuts at: 0 539 680 716 735
Size: 539 141 36 19
Fragments arranged by size:
539 141 36 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 735
Size: 129 32 574
Fragments arranged by size:
574 129 32
XbaI T'CTAG_A
Cuts at: 0 306 735
Size: 306 429
XmnI GAAnn'nnTTC
Cuts at: 0 412 735
Size: 412 323
Enzymes that do cut:
Acc65I AhdI AlwNI ApaI BamHI BanI BanII BbsI
BciVI BglI Bme1580I BplI BpmI BsaI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstYI
BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HindIII KpnI MmeI MslI NcoI NdeI NotI
NspI PfoI PpuMI PspOMI PsrI PstI SacI SanDI
SfcI SfiI StuI StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
MscI MspA1I NaeI NarI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XcmI XhoI XmaI ZraI