Restriction map for
pFN21AE1221
(Linear) (Six-base) MAPSORT of: KIEE1221.seq Check: 6476 from: 1 to: 729
KIEE1221.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 439 612 729
Size: 439 173 117
Fragments arranged by size:
439 173 117
AccI GT'mk_AC
Cuts at: 0 449 476 729
Size: 449 27 253
Fragments arranged by size:
449 253 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 729
Size: 300 429
AfeI AGC'GCT
Cuts at: 0 328 729
Size: 328 401
AlwNI CAG_nnn'CTG
Cuts at: 0 80 729
Size: 80 649
ApaI G_GGCC'C
Cuts at: 0 272 729
Size: 272 457
AscI GG'CGCG_CC
Cuts at: 0 170 729
Size: 170 559
AvaI C'yCGr_G
Cuts at: 0 84 175 227 238 573 729
Size: 84 91 52 11 335 156
Fragments arranged by size:
335 156 91 84 52 11
BanI G'GyrC_C
Cuts at: 0 89 297 342 438 729
Size: 89 208 45 96 291
Fragments arranged by size:
291 208 96 89 45
BanII G_rGCy'C
Cuts at: 0 183 272 729
Size: 183 89 457
Fragments arranged by size:
457 183 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 539 729
Size: 505 34 190
Fragments arranged by size:
505 190 34
BglI GCCn_nnn'nGGC
Cuts at: 0 104 122 149 158 167 276 729
Size: 104 18 27 9 9 109 453
Fragments arranged by size:
453 109 104 27 18 9 9
Bme1580I G_kGCm'C
Cuts at: 0 92 272 729
Size: 92 180 457
Fragments arranged by size:
457 180 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 712 729
Size: 712 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 729
Size: 616 113
BsaHI Gr'CG_yC
Cuts at: 0 410 729
Size: 410 319
BsaWI w'CCGG_w
Cuts at: 0 682 729
Size: 682 47
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 263 729
Size: 1 262 466
Fragments arranged by size:
466 262 1
BseYI C'CCAG_C
Cuts at: 0 73 729
Size: 73 656
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 729
Size: 640 89
BsiEI CG_ry'CG
Cuts at: 0 41 162 378 729
Size: 41 121 216 351
Fragments arranged by size:
351 216 121 41
BsiHKAI G_wGCw'C
Cuts at: 0 295 729
Size: 295 434
Bsp1286I G_dGCh'C
Cuts at: 0 92 183 272 295 729
Size: 92 91 89 23 434
Fragments arranged by size:
434 92 91 89 23
BspEI T'CCGG_A
Cuts at: 0 682 729
Size: 682 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 439 612 729
Size: 439 173 117
Fragments arranged by size:
439 173 117
BsrDI GCAATG_nn'
Cuts at: 0 521 729
Size: 521 208
BsrFI r'CCGG_y
Cuts at: 0 40 418 729
Size: 40 378 311
Fragments arranged by size:
378 311 40
BssHII G'CGCG_C
Cuts at: 0 170 729
Size: 170 559
BstXI CCAn_nnnn'nTGG
Cuts at: 0 442 729
Size: 442 287
BstYI r'GATC_y
Cuts at: 0 68 729
Size: 68 661
Bsu36I CC'TnA_GG
Cuts at: 0 602 729
Size: 602 127
BtgI C'CryG_G
Cuts at: 0 120 263 584 729
Size: 120 143 321 145
Fragments arranged by size:
321 145 143 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 161 729
Size: 161 568
EaeI y'GGCC_r
Cuts at: 0 38 150 159 729
Size: 38 112 9 570
Fragments arranged by size:
570 112 38 9
EagI C'GGCC_G
Cuts at: 0 38 159 729
Size: 38 121 570
Fragments arranged by size:
570 121 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 300 338 729
Size: 300 38 391
Fragments arranged by size:
391 300 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 616 729
Size: 300 316 113
Fragments arranged by size:
316 300 113
EcoO109I rG'GnC_Cy
Cuts at: 0 223 234 259 268 729
Size: 223 11 25 9 461
Fragments arranged by size:
461 223 25 11 9
HaeII r_GCGC'y
Cuts at: 0 323 330 729
Size: 323 7 399
Fragments arranged by size:
399 323 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 729
Size: 725 4
MspA1I CmG'CkG
Cuts at: 0 265 729
Size: 265 464
NaeI GCC'GGC
Cuts at: 0 42 729
Size: 42 687
NcoI C'CATG_G
Cuts at: 0 120 584 729
Size: 120 464 145
Fragments arranged by size:
464 145 120
NgoMIV G'CCGG_C
Cuts at: 0 40 729
Size: 40 689
PfoI T'CCnGG_A
Cuts at: 0 183 729
Size: 183 546
PpuMI rG'GwC_Cy
Cuts at: 0 223 234 259 729
Size: 223 11 25 470
Fragments arranged by size:
470 223 25 11
PspOMI G'GGCC_C
Cuts at: 0 268 729
Size: 268 461
PspXI vC'TCGA_Gb
Cuts at: 0 227 729
Size: 227 502
PstI C_TGCA'G
Cuts at: 0 494 729
Size: 494 235
SacII CC_GC'GG
Cuts at: 0 266 729
Size: 266 463
SanDI GG'GwC_CC
Cuts at: 0 234 259 729
Size: 234 25 470
Fragments arranged by size:
470 234 25
SfcI C'TryA_G
Cuts at: 0 490 729
Size: 490 239
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 149 158 276 729
Size: 149 9 118 453
Fragments arranged by size:
453 149 118 9
SmaI CCC'GGG
Cuts at: 0 86 177 240 729
Size: 86 91 63 489
Fragments arranged by size:
489 91 86 63
SmlI C'TyrA_G
Cuts at: 0 227 729
Size: 227 502
StyI C'CwwG_G
Cuts at: 0 120 584 729
Size: 120 464 145
Fragments arranged by size:
464 145 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 606 616 622 729
Size: 606 10 6 107
Fragments arranged by size:
606 107 10 6
TatI w'GTAC_w
Cuts at: 0 695 729
Size: 695 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 598 630 677 709 729
Size: 31 567 32 47 32 20
Fragments arranged by size:
567 47 32 32 31 20
Tth111I GACn'n_nGTC
Cuts at: 0 412 729
Size: 412 317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 729
Size: 591 138
XhoI C'TCGA_G
Cuts at: 0 227 729
Size: 227 502
XmaI C'CCGG_G
Cuts at: 0 84 175 238 729
Size: 84 91 63 491
Fragments arranged by size:
491 91 84 63
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI AscI AvaI
BanI BanII BcgI BglI Bme1580I BmrI BpmI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrDI BsrFI BssHII BstXI BstYI Bsu36I BtgI
BtgZI EaeI EagI EciI Eco57MI EcoO109I HaeII MmeI
MspA1I NaeI NcoI NgoMIV PfoI PpuMI PspOMI PspXI
PstI SacII SanDI SfcI SfiI SmaI SmlI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrBI BsrGI
BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NarI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XmnI ZraI