Restriction map for
pFN21AE1219
(Linear) (Six-base) MAPSORT of: KIEE1219.seq Check: 1833 from: 1 to: 669
KIEE1219.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AccI GT'mk_AC
Cuts at: 0 467 669
Size: 467 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 669
Size: 237 432
AlwNI CAG_nnn'CTG
Cuts at: 0 147 669
Size: 147 522
ApaLI G'TGCA_C
Cuts at: 0 7 231 669
Size: 7 224 438
Fragments arranged by size:
438 224 7
ApoI r'AATT_y
Cuts at: 0 419 669
Size: 419 250
AvaI C'yCGr_G
Cuts at: 0 132 175 669
Size: 132 43 494
Fragments arranged by size:
494 132 43
BanII G_rGCy'C
Cuts at: 0 190 229 638 669
Size: 190 39 409 31
Fragments arranged by size:
409 190 39 31
BfrBI ATG'CAT
Cuts at: 0 306 669
Size: 306 363
BglI GCCn_nnn'nGGC
Cuts at: 0 31 619 669
Size: 31 588 50
Fragments arranged by size:
588 50 31
BlpI GC'TnA_GC
Cuts at: 0 52 334 637 669
Size: 52 282 303 32
Fragments arranged by size:
303 282 52 32
Bme1580I G_kGCm'C
Cuts at: 0 11 235 669
Size: 11 224 434
Fragments arranged by size:
434 224 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 280 669
Size: 139 141 389
Fragments arranged by size:
389 141 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 428 458 490 520 669
Size: 428 30 32 30 149
Fragments arranged by size:
428 149 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 391 394 532 669
Size: 376 15 3 138 137
Fragments arranged by size:
376 138 137 15 3
BseYI C'CCAG_C
Cuts at: 0 150 454 669
Size: 150 304 215
Fragments arranged by size:
304 215 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 669
Size: 241 428
BsiEI CG_ry'CG
Cuts at: 0 74 669
Size: 74 595
BsiHKAI G_wGCw'C
Cuts at: 0 11 65 190 235 638 669
Size: 11 54 125 45 403 31
Fragments arranged by size:
403 125 54 45 31 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 639 669
Size: 639 30
Bsp1286I G_dGCh'C
Cuts at: 0 11 65 190 229 235 638 669
Size: 11 54 125 39 6 403 31
Fragments arranged by size:
403 125 54 39 31 11 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 220 669
Size: 155 65 449
Fragments arranged by size:
449 155 65
BsrBI CCG'CTC
Cuts at: 0 114 333 669
Size: 114 219 336
Fragments arranged by size:
336 219 114
BsrFI r'CCGG_y
Cuts at: 0 25 287 669
Size: 25 262 382
Fragments arranged by size:
382 262 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 669
Size: 147 522
BstEII G'GTnAC_C
Cuts at: 0 444 669
Size: 444 225
BtgI C'CryG_G
Cuts at: 0 440 669
Size: 440 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 573 669
Size: 573 96
EaeI y'GGCC_r
Cuts at: 0 23 71 669
Size: 23 48 598
Fragments arranged by size:
598 48 23
EagI C'GGCC_G
Cuts at: 0 71 669
Size: 71 598
EarI CTCTTCn'nnn_
Cuts at: 0 430 669
Size: 430 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 222 669
Size: 222 447
EcoICRI GAG'CTC
Cuts at: 0 188 636 669
Size: 188 448 33
Fragments arranged by size:
448 188 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 237 280 669
Size: 139 98 43 389
Fragments arranged by size:
389 139 98 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 669
Size: 535 134
EcoO109I rG'GnC_Cy
Cuts at: 0 144 525 669
Size: 144 381 144
Fragments arranged by size:
381 144 144
EcoRI G'AATT_C
Cuts at: 0 419 669
Size: 419 250
HaeII r_GCGC'y
Cuts at: 0 52 505 669
Size: 52 453 164
Fragments arranged by size:
453 164 52
MspA1I CmG'CkG
Cuts at: 0 240 669
Size: 240 429
NaeI GCC'GGC
Cuts at: 0 27 289 669
Size: 27 262 380
Fragments arranged by size:
380 262 27
NcoI C'CATG_G
Cuts at: 0 440 669
Size: 440 229
NgoMIV G'CCGG_C
Cuts at: 0 25 287 669
Size: 25 262 382
Fragments arranged by size:
382 262 25
NotI GC'GGCC_GC
Cuts at: 0 71 669
Size: 71 598
NsiI A_TGCA'T
Cuts at: 0 308 669
Size: 308 361
PasI CC'CwG_GG
Cuts at: 0 570 669
Size: 570 99
PfoI T'CCnGG_A
Cuts at: 0 494 530 669
Size: 494 36 139
Fragments arranged by size:
494 139 36
PpuMI rG'GwC_Cy
Cuts at: 0 144 669
Size: 144 525
SacI G_AGCT'C
Cuts at: 0 190 638 669
Size: 190 448 31
Fragments arranged by size:
448 190 31
SexAI A'CCwGG_T
Cuts at: 0 536 669
Size: 536 133
SmaI CCC'GGG
Cuts at: 0 134 669
Size: 134 535
StyI C'CwwG_G
Cuts at: 0 440 669
Size: 440 229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 428 460 490 522 669
Size: 428 32 30 32 147
Fragments arranged by size:
428 147 32 32 30
XmaI C'CCGG_G
Cuts at: 0 132 669
Size: 132 537
XmnI GAAnn'nnTTC
Cuts at: 0 250 669
Size: 250 419
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI AvaI BanII BfrBI
BglI BlpI Bme1580I BpmI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstEII BtgI BtgZI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII MspA1I NaeI NcoI
NgoMIV NotI NsiI PasI PfoI PpuMI SacI SexAI
SmaI StyI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BglII BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NarI NdeI NheI NruI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI ZraI