(Linear) (Six-base) MAPSORT of: KIEE1217.seq  Check: 3677  from: 1  to: 597



KIEE1217.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     597 
   Size:        343     254
 
AflIII A'CryG_T

Cuts at:      0     461     597 
   Size:        461     136
 
ApaLI G'TGCA_C

Cuts at:      0     289     597 
   Size:        289     308
 
ApoI r'AATT_y

Cuts at:      0     222     597 
   Size:        222     375
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      41     283     597 
   Size:         41     242     314

  Fragments arranged by size: 

                 314     242      41
 
BbvCI CC'TCA_GC

Cuts at:      0     491     597 
   Size:        491     106
 
BfrBI ATG'CAT

Cuts at:      0     284     597 
   Size:        284     313
 
Bme1580I G_kGCm'C

Cuts at:      0     293     597 
   Size:        293     304
 
Bpu10I CC'TnA_GC

Cuts at:      0     433     491     597 
   Size:        433      58     106

  Fragments arranged by size: 

                 433     106      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     572     597 
   Size:        572      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3     508     597 
   Size:          3     505      89

  Fragments arranged by size: 

                 505      89       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     293     597 
   Size:        293     304
 
Bsp1286I G_dGCh'C

Cuts at:      0     293     597 
   Size:        293     304
 
BsrDI GCAATG_nn'

Cuts at:      0     452     597 
   Size:        452     145
 
BtsI GCAGTG_nn'

Cuts at:      0      21     597 
   Size:         21     576
 
DraIII CAC_nnn'GTG

Cuts at:      0     183     597 
   Size:        183     414
 
EaeI y'GGCC_r

Cuts at:      0     185     597 
   Size:        185     412
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     597 
   Size:        343     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0     393     597 
   Size:        393     204
 
EcoRI G'AATT_C

Cuts at:      0     222     597 
   Size:        222     375
 
HincII GTy'rAC

Cuts at:      0     168     195     411     597 
   Size:        168      27     216     186

  Fragments arranged by size: 

                 216     186     168      27
 
HindIII A'AGCT_T

Cuts at:      0     355     597 
   Size:        355     242
 
MfeI C'AATT_G

Cuts at:      0      53     597 
   Size:         53     544
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     502     597 
   Size:        502      95
 
MscI TGG'CCA

Cuts at:      0     187     597 
   Size:        187     410
 
NsiI A_TGCA'T

Cuts at:      0     286     597 
   Size:        286     311
 
NspI r_CATG'y

Cuts at:      0     284     465     597 
   Size:        284     181     132

  Fragments arranged by size: 

                 284     181     132
 
PciI A'CATG_T

Cuts at:      0     461     597 
   Size:        461     136
 
PfoI T'CCnGG_A

Cuts at:      0     217     597 
   Size:        217     380
 
StyI C'CwwG_G

Cuts at:      0     389     597 
   Size:        389     208
 
TatI w'GTAC_w

Cuts at:      0     401     549     597 
   Size:        401     148      48

  Fragments arranged by size: 

                 401     148      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     572     597 
   Size:        572      25
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI     BbsI    BbvCI    BfrBI Bme1580I
   Bpu10I    BsaXI    BseRI  BsiHKAI Bsp1286I    BsrDI     BtsI   DraIII
     EaeI  Eco57MI EcoO109I    EcoRI   HincII  HindIII     MfeI     MmeI
     MscI     NsiI     NspI     PciI     PfoI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
     HpaI     KasI     KpnI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI