(Linear) (Six-base) MAPSORT of: KIEE1215.seq  Check: 2564  from: 1  to: 408



KIEE1215.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AccI GT'mk_AC

Cuts at:      0     172     408 
   Size:        172     236
 
AgeI A'CCGG_T

Cuts at:      0      34     408 
   Size:         34     374
 
BanI G'GyrC_C

Cuts at:      0     133     408 
   Size:        133     275
 
BanII G_rGCy'C

Cuts at:      0     311     408 
   Size:        311      97
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     162     196     408 
   Size:        162      34     212

  Fragments arranged by size: 

                 212     162      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     312     408 
   Size:        312      96
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     292     408 
   Size:         70     222     116

  Fragments arranged by size: 

                 222     116      70
 
BglII A'GATC_T

Cuts at:      0     243     408 
   Size:        243     165
 
BlpI GC'TnA_GC

Cuts at:      0     344     408 
   Size:        344      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     227     408 
   Size:        227     181
 
BsaWI w'CCGG_w

Cuts at:      0      34     408 
   Size:         34     374
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     408 
   Size:          5     403
 
BsmI GAATG_Cn'

Cuts at:      0     389     408 
   Size:        389      19
 
Bsp1286I G_dGCh'C

Cuts at:      0     311     408 
   Size:        311      97
 
BsrFI r'CCGG_y

Cuts at:      0      34      90      97     408 
   Size:         34      56       7     311

  Fragments arranged by size: 

                 311      56      34       7
 
BssHII G'CGCG_C

Cuts at:      0      86     408 
   Size:         86     322
 
BstYI r'GATC_y

Cuts at:      0     243     408 
   Size:        243     165
 
BtgI C'CryG_G

Cuts at:      0     137     263     408 
   Size:        137     126     145

  Fragments arranged by size: 

                 145     137     126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     103     408 
   Size:        103     305
 
EaeI y'GGCC_r

Cuts at:      0      62      92     408 
   Size:         62      30     316

  Fragments arranged by size: 

                 316      62      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     280     408 
   Size:        280     128
 
HaeII r_GCGC'y

Cuts at:      0      49     408 
   Size:         49     359
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     341     373     408 
   Size:        341      32      35

  Fragments arranged by size: 

                 341      35      32
 
HincII GTy'rAC

Cuts at:      0     173     408 
   Size:        173     235
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     228     408 
   Size:        228     180
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MspA1I CmG'CkG

Cuts at:      0      60     265     408 
   Size:         60     205     143

  Fragments arranged by size: 

                 205     143      60
 
NaeI GCC'GGC

Cuts at:      0      92     408 
   Size:         92     316
 
NgoMIV G'CCGG_C

Cuts at:      0      90     408 
   Size:         90     318
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210     408 
   Size:        210     198
 
PstI C_TGCA'G

Cuts at:      0     281     408 
   Size:        281     127
 
PvuII CAG'CTG

Cuts at:      0      60     408 
   Size:         60     348
 
SacII CC_GC'GG

Cuts at:      0     266     408 
   Size:        266     142
 
SalI G'TCGA_C

Cuts at:      0     171     408 
   Size:        171     237
 
SbfI CC_TGCA'GG

Cuts at:      0     281     408 
   Size:        281     127
 
SfcI C'TryA_G

Cuts at:      0     277     408 
   Size:        277     131
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     292     408 
   Size:        292     116
 
SgrAI Cr'CCGG_yG

Cuts at:      0      34      97     408 
   Size:         34      63     311

  Fragments arranged by size: 

                 311      63      34
 
StuI AGG'CCT

Cuts at:      0     286     295     408 
   Size:        286       9     113

  Fragments arranged by size: 

                 286     113       9
 
StyI C'CwwG_G

Cuts at:      0     365     408 
   Size:        365      43
 

 Enzymes that do cut:

     AccI     AgeI     BanI    BanII     BcgI    BciVI     BglI    BglII
     BlpI   Bpu10I    BsaWI     BsgI     BsmI Bsp1286I    BsrFI   BssHII
    BstYI     BtgI    BtgZI     EaeI    EcoNI    HaeII    Hin4I   HincII
     MmeI     MscI   MspA1I     NaeI   NgoMIV    PflMI     PstI    PvuII
    SacII     SalI     SbfI     SfcI     SfiI    SgrAI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BclI    BfrBI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SanDI     SapI     ScaI    SexAI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI