Restriction map for
pFN21AE1209
(Linear) (Six-base) MAPSORT of: KIEE1209.seq Check: 5561 from: 1 to: 390
KIEE1209.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 257 390
Size: 257 133
AgeI A'CCGG_T
Cuts at: 0 95 390
Size: 95 295
ApaLI G'TGCA_C
Cuts at: 0 91 390
Size: 91 299
AvaI C'yCGr_G
Cuts at: 0 166 390
Size: 166 224
BanI G'GyrC_C
Cuts at: 0 133 139 390
Size: 133 6 251
Fragments arranged by size:
251 133 6
BanII G_rGCy'C
Cuts at: 0 281 390
Size: 281 109
BbeI G_GCGC'C
Cuts at: 0 137 143 390
Size: 137 6 247
Fragments arranged by size:
247 137 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 364 390
Size: 232 132 26
Fragments arranged by size:
232 132 26
Bme1580I G_kGCm'C
Cuts at: 0 95 390
Size: 95 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 390
Size: 211 179
BsaHI Gr'CG_yC
Cuts at: 0 134 140 320 390
Size: 134 6 180 70
Fragments arranged by size:
180 134 70 6
BsaWI w'CCGG_w
Cuts at: 0 4 95 390
Size: 4 91 295
Fragments arranged by size:
295 91 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 390
Size: 289 101
BseYI C'CCAG_C
Cuts at: 0 291 390
Size: 291 99
BsiEI CG_ry'CG
Cuts at: 0 9 390
Size: 9 381
BsiHKAI G_wGCw'C
Cuts at: 0 95 167 281 390
Size: 95 72 114 109
Fragments arranged by size:
114 109 95 72
Bsp1286I G_dGCh'C
Cuts at: 0 95 167 281 390
Size: 95 72 114 109
Fragments arranged by size:
114 109 95 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 257 390
Size: 257 133
BsrFI r'CCGG_y
Cuts at: 0 95 136 199 390
Size: 95 41 63 191
Fragments arranged by size:
191 95 63 41
BssHII G'CGCG_C
Cuts at: 0 31 33 60 211 390
Size: 31 2 27 151 179
Fragments arranged by size:
179 151 31 27 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 390
Size: 99 291
BstYI r'GATC_y
Cuts at: 0 185 390
Size: 185 205
BtgI C'CryG_G
Cuts at: 0 80 158 390
Size: 80 78 232
Fragments arranged by size:
232 80 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 291 390
Size: 291 99
EaeI y'GGCC_r
Cuts at: 0 155 257 390
Size: 155 102 133
Fragments arranged by size:
155 133 102
EcoICRI GAG'CTC
Cuts at: 0 279 390
Size: 279 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 390
Size: 211 179
HaeII r_GCGC'y
Cuts at: 0 137 143 390
Size: 137 6 247
Fragments arranged by size:
247 137 6
KasI G'GCGC_C
Cuts at: 0 133 139 390
Size: 133 6 251
Fragments arranged by size:
251 133 6
MscI TGG'CCA
Cuts at: 0 259 390
Size: 259 131
MspA1I CmG'CkG
Cuts at: 0 160 255 390
Size: 160 95 135
Fragments arranged by size:
160 135 95
NaeI GCC'GGC
Cuts at: 0 138 201 390
Size: 138 63 189
Fragments arranged by size:
189 138 63
NarI GG'CG_CC
Cuts at: 0 134 140 390
Size: 134 6 250
Fragments arranged by size:
250 134 6
NgoMIV G'CCGG_C
Cuts at: 0 136 199 390
Size: 136 63 191
Fragments arranged by size:
191 136 63
PfoI T'CCnGG_A
Cuts at: 0 188 390
Size: 188 202
PspXI vC'TCGA_Gb
Cuts at: 0 166 390
Size: 166 224
PstI C_TGCA'G
Cuts at: 0 74 254 390
Size: 74 180 136
Fragments arranged by size:
180 136 74
PvuII CAG'CTG
Cuts at: 0 255 390
Size: 255 135
SacI G_AGCT'C
Cuts at: 0 281 390
Size: 281 109
SacII CC_GC'GG
Cuts at: 0 161 390
Size: 161 229
ScaI AGT'ACT
Cuts at: 0 172 390
Size: 172 218
SfcI C'TryA_G
Cuts at: 0 70 250 390
Size: 70 180 140
Fragments arranged by size:
180 140 70
SfoI GGC'GCC
Cuts at: 0 135 141 390
Size: 135 6 249
Fragments arranged by size:
249 135 6
SgrAI Cr'CCGG_yG
Cuts at: 0 136 390
Size: 136 254
SmlI C'TyrA_G
Cuts at: 0 166 390
Size: 166 224
StyI C'CwwG_G
Cuts at: 0 38 380 390
Size: 38 342 10
Fragments arranged by size:
342 38 10
TatI w'GTAC_w
Cuts at: 0 170 390
Size: 170 220
XhoI C'TCGA_G
Cuts at: 0 166 390
Size: 166 224
Enzymes that do cut:
AarI AgeI ApaLI AvaI BanI BanII BbeI BbsI
Bme1580I BpmI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BssHII BstAPI BstYI BtgI BtgZI
EaeI EcoICRI Eco57MI HaeII KasI MscI MspA1I NaeI
NarI NgoMIV PfoI PspXI PstI PvuII SacI SacII
ScaI SfcI SfoI SgrAI SmlI StyI TatI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PvuI RsrII SalI SanDI SapI
SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI XmnI ZraI