(Linear) (Six-base) MAPSORT of: KIEE1206.seq  Check: 5761  from: 1  to: 378



KIEE1206.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     214     378 
   Size:        214     164
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
AfeI AGC'GCT

Cuts at:      0     259     372     378 
   Size:        259     113       6

  Fragments arranged by size: 

                 259     113       6
 
ApoI r'AATT_y

Cuts at:      0     189     378 
   Size:        189     189
 
BanII G_rGCy'C

Cuts at:      0      43     378 
   Size:         43     335
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     378 
   Size:        258     120
 
BsiEI CG_ry'CG

Cuts at:      0     293     378 
   Size:        293      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     378 
   Size:         43     335
 
BspHI T'CATG_A

Cuts at:      0     185     378 
   Size:        185     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132     214     378 
   Size:        132      82     164

  Fragments arranged by size: 

                 164     132      82
 
BsrFI r'CCGG_y

Cuts at:      0     157     378 
   Size:        157     221
 
BsrGI T'GTAC_A

Cuts at:      0     125     378 
   Size:        125     253
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     378 
   Size:        177     201
 
BstYI r'GATC_y

Cuts at:      0     281     378 
   Size:        281      97
 
BtgI C'CryG_G

Cuts at:      0     176     378 
   Size:        176     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204     378 
   Size:        204     174
 
DraIII CAC_nnn'GTG

Cuts at:      0     333     378 
   Size:        333      45
 
EaeI y'GGCC_r

Cuts at:      0     290     320     378 
   Size:        290      30      58

  Fragments arranged by size: 

                 290      58      30
 
EagI C'GGCC_G

Cuts at:      0     290     378 
   Size:        290      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     258     378 
   Size:        157     101     120

  Fragments arranged by size: 

                 157     120     101
 
EcoRI G'AATT_C

Cuts at:      0     189     378 
   Size:        189     189
 
HaeII r_GCGC'y

Cuts at:      0     261     374     378 
   Size:        261     113       4

  Fragments arranged by size: 

                 261     113       4
 
MscI TGG'CCA

Cuts at:      0     322     378 
   Size:        322      56
 
MslI CAynn'nnrTG

Cuts at:      0     121     378 
   Size:        121     257
 
NcoI C'CATG_G

Cuts at:      0     176     378 
   Size:        176     202
 
StyI C'CwwG_G

Cuts at:      0     170     176     378 
   Size:        170       6     202

  Fragments arranged by size: 

                 202     170       6
 
TatI w'GTAC_w

Cuts at:      0     125     362     378 
   Size:        125     237      16

  Fragments arranged by size: 

                 237     125      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351     378 
   Size:        319      32      27

  Fragments arranged by size: 

                 319      32      27
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     ApoI    BanII     BpmI    BsiEI Bsp1286I
    BspHI    BspMI    BsrFI    BsrGI    BstXI    BstYI     BtgI    BtgZI
   DraIII     EaeI     EagI  Eco57MI    EcoRI    HaeII     MscI     MslI
     NcoI     StyI     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI     DrdI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI