(Linear) (Six-base) MAPSORT of: KIEE1204.seq  Check: 6355  from: 1  to: 345



KIEE1204.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

ApaI G_GGCC'C

Cuts at:      0      63     345 
   Size:         63     282
 
BanII G_rGCy'C

Cuts at:      0      63     300     345 
   Size:         63     237      45

  Fragments arranged by size: 

                 237      63      45
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     280     345 
   Size:        280      65
 
BglI GCCn_nnn'nGGC

Cuts at:      0     178     345 
   Size:        178     167
 
Bme1580I G_kGCm'C

Cuts at:      0      63     345 
   Size:         63     282
 
BmgBI CAC'GTC

Cuts at:      0      75     345 
   Size:         75     270
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     302     345 
   Size:        302      43
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     241     332     345 
   Size:         26     215      91      13

  Fragments arranged by size: 

                 215      91      26      13
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      24     345 
   Size:         24     321
 
BsaBI GATnn'nnATC

Cuts at:      0     218     345 
   Size:        218     127
 
BsaWI w'CCGG_w

Cuts at:      0      52     345 
   Size:         52     293
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     345 
   Size:        103     242
 
BseYI C'CCAG_C

Cuts at:      0     100     345 
   Size:        100     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     153     345 
   Size:        153     192
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     153     300     345 
   Size:         63      90     147      45

  Fragments arranged by size: 

                 147      90      63      45
 
BspEI T'CCGG_A

Cuts at:      0      52     345 
   Size:         52     293
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270     345 
   Size:        270      75
 
BtgI C'CryG_G

Cuts at:      0      14     345 
   Size:         14     331
 
EarI CTCTTCn'nnn_

Cuts at:      0      73     345 
   Size:         73     272
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     241     332     345 
   Size:         26     215      91      13

  Fragments arranged by size: 

                 215      91      26      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     252     345 
   Size:        252      93
 
HaeII r_GCGC'y

Cuts at:      0      13     121     345 
   Size:         13     108     224

  Fragments arranged by size: 

                 224     108      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      64      96     345 
   Size:         64      32     249

  Fragments arranged by size: 

                 249      64      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     311     345 
   Size:        122     189      34

  Fragments arranged by size: 

                 189     122      34
 
MslI CAynn'nnrTG

Cuts at:      0     130     345 
   Size:        130     215
 
MspA1I CmG'CkG

Cuts at:      0     100     169     270     345 
   Size:        100      69     101      75

  Fragments arranged by size: 

                 101     100      75      69
 
NruI TCG'CGA

Cuts at:      0      86     345 
   Size:         86     259
 
PasI CC'CwG_GG

Cuts at:      0     112     345 
   Size:        112     233
 
PspOMI G'GGCC_C

Cuts at:      0      59     345 
   Size:         59     286
 
PstI C_TGCA'G

Cuts at:      0     158     345 
   Size:        158     187
 
PvuII CAG'CTG

Cuts at:      0     100     169     270     345 
   Size:        100      69     101      75

  Fragments arranged by size: 

                 101     100      75      69
 
SapI GCTCTTCn'nnn_

Cuts at:      0      73     345 
   Size:         73     272
 
SfcI C'TryA_G

Cuts at:      0     154     318     345 
   Size:        154     164      27

  Fragments arranged by size: 

                 164     154      27
 

 Enzymes that do cut:

     ApaI    BanII    BciVI     BglI Bme1580I    BmgBI     BmrI     BpmI
     BsaI    BsaBI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI
    BstXI     BtgI     EarI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI
     MslI   MspA1I     NruI     PasI   PspOMI     PstI    PvuII     SapI
     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII
     BlpI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI