(Linear) (Six-base) MAPSORT of: KIEE1202.seq  Check: 7391  from: 1  to: 333



KIEE1202.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     333 
   Size:        140     193
 
ApoI r'AATT_y

Cuts at:      0      95     236     333 
   Size:         95     141      97

  Fragments arranged by size: 

                 141      97      95
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     290     333 
   Size:        290      43
 
BfrBI ATG'CAT

Cuts at:      0     129     333 
   Size:        129     204
 
BmgBI CAC'GTC

Cuts at:      0     193     333 
   Size:        193     140
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      57     333 
   Size:         57     276
 
BsrDI GCAATG_nn'

Cuts at:      0     155     333 
   Size:        155     178
 
EarI CTCTTCn'nnn_

Cuts at:      0     116     333 
   Size:        116     217
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     333 
   Size:        140     193
 
MfeI C'AATT_G

Cuts at:      0      46     333 
   Size:         46     287
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     333 
   Size:         72     261
 
MspA1I CmG'CkG

Cuts at:      0     184     333 
   Size:        184     149
 
NsiI A_TGCA'T

Cuts at:      0     131     333 
   Size:        131     202
 
NspI r_CATG'y

Cuts at:      0     133     333 
   Size:        133     200
 
PvuII CAG'CTG

Cuts at:      0     184     333 
   Size:        184     149
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BfrBI    BmgBI     BsaI    BsrDI     EarI
  Eco57MI     MfeI     MmeI   MspA1I     NsiI     NspI    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI