Restriction map for
pFN21AE1197
(Linear) (Six-base) MAPSORT of: KIEE1197.seq Check: 6523 from: 1 to: 2751
KIEE1197.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 29 1027 2751
Size: 29 998 1724
Fragments arranged by size:
1724 998 29
Acc65I G'GTAC_C
Cuts at: 0 1499 2751
Size: 1499 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 929 1775 1906 2415 2751
Size: 565 364 846 131 509 336
Fragments arranged by size:
846 565 509 364 336 131
AfeI AGC'GCT
Cuts at: 0 990 2751
Size: 990 1761
AflII C'TTAA_G
Cuts at: 0 519 2751
Size: 519 2232
AflIII A'CryG_T
Cuts at: 0 1717 2133 2178 2751
Size: 1717 416 45 573
Fragments arranged by size:
1717 573 416 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 448 2615 2751
Size: 448 2167 136
Fragments arranged by size:
2167 448 136
AlwNI CAG_nnn'CTG
Cuts at: 0 75 403 444 1193 1458 1968 2751
Size: 75 328 41 749 265 510 783
Fragments arranged by size:
783 749 510 328 265 75 41
ApaI G_GGCC'C
Cuts at: 0 115 505 1567 2751
Size: 115 390 1062 1184
Fragments arranged by size:
1184 1062 390 115
ApoI r'AATT_y
Cuts at: 0 1807 2401 2467 2751
Size: 1807 594 66 284
Fragments arranged by size:
1807 594 284 66
AvaI C'yCGr_G
Cuts at: 0 108 379 642 2751
Size: 108 271 263 2109
Fragments arranged by size:
2109 271 263 108
AvrII C'CTAG_G
Cuts at: 0 972 2751
Size: 972 1779
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1753 1786 2751
Size: 1753 33 965
Fragments arranged by size:
1753 965 33
BanI G'GyrC_C
Cuts at: 0 119 151 1499 2357 2751
Size: 119 32 1348 858 394
Fragments arranged by size:
1348 858 394 119 32
BanII G_rGCy'C
Cuts at: 0 115 505 651 1006 1567 1713 1878 2225
Size: 115 390 146 355 561 146 165 347
Cuts at: 2225 2751
Size: 526
Fragments arranged by size:
561 526 390 355 347 165 146 146
115
BbeI G_GCGC'C
Cuts at: 0 155 2751
Size: 155 2596
BbsI GAAGACnn'nnnn_
Cuts at: 0 1669 1728 1831 2751
Size: 1669 59 103 920
Fragments arranged by size:
1669 920 103 59
BbvCI CC'TCA_GC
Cuts at: 0 1281 2751
Size: 1281 1470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1051 1085 2065 2099 2592 2626 2751
Size: 1051 34 980 34 493 34 125
Fragments arranged by size:
1051 980 493 125 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 615 2751
Size: 615 2136
BglI GCCn_nnn'nGGC
Cuts at: 0 314 415 646 859 1198 1244 2751
Size: 314 101 231 213 339 46 1507
Fragments arranged by size:
1507 339 314 231 213 101 46
Bme1580I G_kGCm'C
Cuts at: 0 115 505 685 855 1529 1567 2705 2751
Size: 115 390 180 170 674 38 1138 46
Fragments arranged by size:
1138 674 390 180 170 115 46 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1529 2122 2751
Size: 1529 593 629
Fragments arranged by size:
1529 629 593
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 2706 2751
Size: 486 2220 45
Fragments arranged by size:
2220 486 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 425 1186 2253 2751
Size: 388 37 761 1067 498
Fragments arranged by size:
1067 761 498 388 37
Bpu10I CC'TnA_GC
Cuts at: 0 553 1281 2751
Size: 553 728 1470
Fragments arranged by size:
1470 728 553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2351 2535 2751
Size: 2351 184 216
Fragments arranged by size:
2351 216 184
BsaAI yAC'GTr
Cuts at: 0 2136 2181 2751
Size: 2136 45 570
Fragments arranged by size:
2136 570 45
BsaHI Gr'CG_yC
Cuts at: 0 152 2751
Size: 152 2599
BsaWI w'CCGG_w
Cuts at: 0 456 1048 1585 2667 2751
Size: 456 592 537 1082 84
Fragments arranged by size:
1082 592 537 456 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 2751
Size: 398 30 2323
Fragments arranged by size:
2323 398 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 74 1966 2751
Size: 74 1892 785
Fragments arranged by size:
1892 785 74
BseYI C'CCAG_C
Cuts at: 0 18 498 2751
Size: 18 480 2253
Fragments arranged by size:
2253 480 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1274 2611 2751
Size: 28 1246 1337 140
Fragments arranged by size:
1337 1246 140 28
BsiEI CG_ry'CG
Cuts at: 0 40 2511 2751
Size: 40 2471 240
Fragments arranged by size:
2471 240 40
BsiHKAI G_wGCw'C
Cuts at: 0 907 1006 1056 1713 2751
Size: 907 99 50 657 1038
Fragments arranged by size:
1038 907 657 99 50
BsmI GAATG_Cn'
Cuts at: 0 871 2751
Size: 871 1880
Bsp1286I G_dGCh'C
Cuts at: 0 115 505 651 685 855 907 1006 1056
Size: 115 390 146 34 170 52 99 50
Cuts at: 1056 1529 1567 1713 1878 2225 2705 2751
Size: 473 38 146 165 347 480 46
Fragments arranged by size:
480 473 390 347 170 165 146 146
115 99 52 50 46 38 34
BspEI T'CCGG_A
Cuts at: 0 456 1048 1585 2667 2751
Size: 456 592 537 1082 84
Fragments arranged by size:
1082 592 537 456 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1675 2639 2751
Size: 1675 964 112
Fragments arranged by size:
1675 964 112
BsrDI GCAATG_nn'
Cuts at: 0 2516 2751
Size: 2516 235
BsrGI T'GTAC_A
Cuts at: 0 2130 2333 2751
Size: 2130 203 418
Fragments arranged by size:
2130 418 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 444 2751
Size: 444 2307
BstBI TT'CG_AA
Cuts at: 0 2484 2751
Size: 2484 267
BstEII G'GTnAC_C
Cuts at: 0 1274 2751
Size: 1274 1477
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1339 2580 2751
Size: 744 595 1241 171
Fragments arranged by size:
1241 744 595 171
BstYI r'GATC_y
Cuts at: 0 2708 2751
Size: 2708 43
Bsu36I CC'TnA_GG
Cuts at: 0 1451 2751
Size: 1451 1300
BtgI C'CryG_G
Cuts at: 0 413 737 2237 2751
Size: 413 324 1500 514
Fragments arranged by size:
1500 514 413 324
BtsI GCAGTG_nn'
Cuts at: 0 731 1219 2419 2751
Size: 731 488 1200 332
Fragments arranged by size:
1200 731 488 332
DraIII CAC_nnn'GTG
Cuts at: 0 2652 2751
Size: 2652 99
EaeI y'GGCC_r
Cuts at: 0 37 416 860 1112 2751
Size: 37 379 444 252 1639
Fragments arranged by size:
1639 444 379 252 37
EagI C'GGCC_G
Cuts at: 0 37 2751
Size: 37 2714
EarI CTCTTCn'nnn_
Cuts at: 0 1318 2434 2751
Size: 1318 1116 317
Fragments arranged by size:
1318 1116 317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1121 2751
Size: 1121 1630
EcoICRI GAG'CTC
Cuts at: 0 1004 1711 2751
Size: 1004 707 1040
Fragments arranged by size:
1040 1004 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 425 565 929 1186 1775 1906 2253
Size: 388 37 140 364 257 589 131 347
Cuts at: 2253 2415 2751
Size: 162 336
Fragments arranged by size:
589 388 364 347 336 257 162 140
131 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 556 2751
Size: 331 225 2195
Fragments arranged by size:
2195 331 225
EcoO109I rG'GnC_Cy
Cuts at: 0 112 307 502 1227 1261 1564 2751
Size: 112 195 195 725 34 303 1187
Fragments arranged by size:
1187 725 303 195 195 112 34
EcoRI G'AATT_C
Cuts at: 0 2401 2751
Size: 2401 350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1755 2751
Size: 1755 996
HaeII r_GCGC'y
Cuts at: 0 155 992 1193 2751
Size: 155 837 201 1558
Fragments arranged by size:
1558 837 201 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 377 409 1567 1599 2751
Size: 377 32 1158 32 1152
Fragments arranged by size:
1158 1152 377 32 32
HincII GTy'rAC
Cuts at: 0 2265 2751
Size: 2265 486
HindIII A'AGCT_T
Cuts at: 0 1688 2751
Size: 1688 1063
KasI G'GCGC_C
Cuts at: 0 151 2751
Size: 151 2600
KpnI G_GTAC'C
Cuts at: 0 1503 2751
Size: 1503 1248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 2751
Size: 740 2011
MslI CAynn'nnrTG
Cuts at: 0 2692 2751
Size: 2692 59
MspA1I CmG'CkG
Cuts at: 0 444 475 527 535 623 832 1069 1176
Size: 444 31 52 8 88 209 237 107
Cuts at: 1176 1258 1753 2751
Size: 82 495 998
Fragments arranged by size:
998 495 444 237 209 107 88 82
52 31 8
NarI GG'CG_CC
Cuts at: 0 152 2751
Size: 152 2599
NcoI C'CATG_G
Cuts at: 0 737 2237 2751
Size: 737 1500 514
Fragments arranged by size:
1500 737 514
NruI TCG'CGA
Cuts at: 0 1085 2751
Size: 1085 1666
NspI r_CATG'y
Cuts at: 0 804 1605 1721 2319 2525 2751
Size: 804 801 116 598 206 226
Fragments arranged by size:
804 801 598 226 206 116
PciI A'CATG_T
Cuts at: 0 1717 2751
Size: 1717 1034
PflMI CCAn_nnn'nTGG
Cuts at: 0 403 2243 2449 2751
Size: 403 1840 206 302
Fragments arranged by size:
1840 403 302 206
PfoI T'CCnGG_A
Cuts at: 0 326 1006 2021 2212 2269 2326 2579 2751
Size: 326 680 1015 191 57 57 253 172
Fragments arranged by size:
1015 680 326 253 191 172 57 57
PmlI CAC'GTG
Cuts at: 0 2136 2181 2751
Size: 2136 45 570
Fragments arranged by size:
2136 570 45
PpuMI rG'GwC_Cy
Cuts at: 0 1227 1261 2751
Size: 1227 34 1490
Fragments arranged by size:
1490 1227 34
PspOMI G'GGCC_C
Cuts at: 0 111 501 1563 2751
Size: 111 390 1062 1188
Fragments arranged by size:
1188 1062 390 111
PstI C_TGCA'G
Cuts at: 0 173 996 2751
Size: 173 823 1755
Fragments arranged by size:
1755 823 173
PvuI CG_AT'CG
Cuts at: 0 2511 2751
Size: 2511 240
PvuII CAG'CTG
Cuts at: 0 444 475 527 1069 2751
Size: 444 31 52 542 1682
Fragments arranged by size:
1682 542 444 52 31
RsrII CG'GwC_CG
Cuts at: 0 1117 2751
Size: 1117 1634
SacI G_AGCT'C
Cuts at: 0 1006 1713 2751
Size: 1006 707 1038
Fragments arranged by size:
1038 1006 707
SanDI GG'GwC_CC
Cuts at: 0 1261 2751
Size: 1261 1490
ScaI AGT'ACT
Cuts at: 0 2209 2751
Size: 2209 542
SexAI A'CCwGG_T
Cuts at: 0 320 2751
Size: 320 2431
SfcI C'TryA_G
Cuts at: 0 169 992 1421 1909 2751
Size: 169 823 429 488 842
Fragments arranged by size:
842 823 488 429 169
SfoI GGC'GCC
Cuts at: 0 153 2751
Size: 153 2598
SmaI CCC'GGG
Cuts at: 0 110 2751
Size: 110 2641
SmlI C'TyrA_G
Cuts at: 0 519 2366 2550 2751
Size: 519 1847 184 201
Fragments arranged by size:
1847 519 201 184
SphI G_CATG'C
Cuts at: 0 2319 2525 2751
Size: 2319 206 226
Fragments arranged by size:
2319 226 206
StuI AGG'CCT
Cuts at: 0 640 2155 2751
Size: 640 1515 596
Fragments arranged by size:
1515 640 596
StyI C'CwwG_G
Cuts at: 0 662 707 737 972 2150 2237 2751
Size: 662 45 30 235 1178 87 514
Fragments arranged by size:
1178 662 514 235 87 45 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 137 736 1685 2751
Size: 137 599 949 1066
Fragments arranged by size:
1066 949 599 137
TatI w'GTAC_w
Cuts at: 0 2130 2207 2333 2751
Size: 2130 77 126 418
Fragments arranged by size:
2130 418 126 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 2392 2424 2751
Size: 398 32 1962 32 327
Fragments arranged by size:
1962 398 327 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1974 2751
Size: 1974 777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 2751
Size: 318 2433
XmaI C'CCGG_G
Cuts at: 0 108 2751
Size: 108 2643
XmnI GAAnn'nnTTC
Cuts at: 0 76 785 2751
Size: 76 709 1966
Fragments arranged by size:
1966 709 76
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AlfI AlwNI
ApaI ApoI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI
NruI NspI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SanDI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI BamHI BclI BfrBI BglII BlpI
BmgBI BmtI BsaI BsaBI BsiWI BsmBI BspHI BsrBI
BsrFI BssHII BssSI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRV FseI FspI FspAI HpaI MfeI MluI
MscI NaeI NdeI NgoMIV NheI NotI NsiI PacI
PasI PmeI PpiI PshAI PsiI PspXI PsrI SacII
SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI ZraI