Restriction map for
pFN21AE1191
(Linear) (Six-base) MAPSORT of: KIEE1191.seq Check: 2010 from: 1 to: 2472
KIEE1191.orf
With 182 enzymes: SgfI *
July 3, 2012 16:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1802 1956 2472
Size: 1802 154 516
Fragments arranged by size:
1802 516 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1647 1980 2472
Size: 1647 333 492
Fragments arranged by size:
1647 492 333
AgeI A'CCGG_T
Cuts at: 0 941 2472
Size: 941 1531
AhdI GACnn_n'nnGTC
Cuts at: 0 956 2104 2472
Size: 956 1148 368
Fragments arranged by size:
1148 956 368
AlwNI CAG_nnn'CTG
Cuts at: 0 906 1089 1437 1794 2472
Size: 906 183 348 357 678
Fragments arranged by size:
906 678 357 348 183
ApaI G_GGCC'C
Cuts at: 0 1747 2472
Size: 1747 725
ApaLI G'TGCA_C
Cuts at: 0 1035 2472
Size: 1035 1437
ApoI r'AATT_y
Cuts at: 0 250 381 390 541 565 576 748 778
Size: 250 131 9 151 24 11 172 30
Cuts at: 778 2472
Size: 1694
Fragments arranged by size:
1694 250 172 151 131 30 24 11
9
AvaI C'yCGr_G
Cuts at: 0 1021 1197 1363 1735 2053 2472
Size: 1021 176 166 372 318 419
Fragments arranged by size:
1021 419 372 318 176 166
BamHI G'GATC_C
Cuts at: 0 1541 2472
Size: 1541 931
BanI G'GyrC_C
Cuts at: 0 651 1192 2298 2472
Size: 651 541 1106 174
Fragments arranged by size:
1106 651 541 174
BanII G_rGCy'C
Cuts at: 0 95 884 1150 1370 1580 1747 2472
Size: 95 789 266 220 210 167 725
Fragments arranged by size:
789 725 266 220 210 167 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 2472
Size: 1106 1366
BbvCI CC'TCA_GC
Cuts at: 0 1128 2472
Size: 1128 1344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1174 1208 1466 1500 2472
Size: 1174 34 258 34 972
Fragments arranged by size:
1174 972 258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 811 1662 2472
Size: 811 851 810
Fragments arranged by size:
851 811 810
BglII A'GATC_T
Cuts at: 0 1763 2472
Size: 1763 709
BlpI GC'TnA_GC
Cuts at: 0 1369 1688 2026 2472
Size: 1369 319 338 446
Fragments arranged by size:
1369 446 338 319
Bme1580I G_kGCm'C
Cuts at: 0 1039 1747 2472
Size: 1039 708 725
Fragments arranged by size:
1039 725 708
BmgBI CAC'GTC
Cuts at: 0 1188 2472
Size: 1188 1284
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 53 2472
Size: 53 2419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 2440 2472
Size: 898 1542 32
Fragments arranged by size:
1542 898 32
Bpu10I CC'TnA_GC
Cuts at: 0 1128 1657 2014 2472
Size: 1128 529 357 458
Fragments arranged by size:
1128 529 458 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1218 2472
Size: 1134 84 1254
Fragments arranged by size:
1254 1134 84
BsaHI Gr'CG_yC
Cuts at: 0 1228 2472
Size: 1228 1244
BsaWI w'CCGG_w
Cuts at: 0 941 1559 2472
Size: 941 618 913
Fragments arranged by size:
941 913 618
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1269 1299 1418 1448 1452 1482 2472
Size: 1269 30 119 30 4 30 990
Fragments arranged by size:
1269 990 119 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1116 1758 2472
Size: 1116 642 714
Fragments arranged by size:
1116 714 642
BseYI C'CCAG_C
Cuts at: 0 257 837 1673 1730 1984 2215 2391 2402
Size: 257 580 836 57 254 231 176 11
Cuts at: 2402 2472
Size: 70
Fragments arranged by size:
836 580 257 254 231 176 70 57
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2237 2472
Size: 2237 235
BsiEI CG_ry'CG
Cuts at: 0 1872 2472
Size: 1872 600
BsiHKAI G_wGCw'C
Cuts at: 0 95 804 884 1039 1462 1580 2210 2472
Size: 95 709 80 155 423 118 630 262
Fragments arranged by size:
709 630 423 262 155 118 95 80
Bsp1286I G_dGCh'C
Cuts at: 0 95 804 884 1039 1150 1370 1462 1580
Size: 95 709 80 155 111 220 92 118
Cuts at: 1580 1747 2210 2472
Size: 167 463 262
Fragments arranged by size:
709 463 262 220 167 155 118 111
95 92 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 830 1802 1956 2280 2294 2472
Size: 830 972 154 324 14 178
Fragments arranged by size:
972 830 324 178 154 14
BsrBI CCG'CTC
Cuts at: 0 1468 1874 2472
Size: 1468 406 598
Fragments arranged by size:
1468 598 406
BsrDI GCAATG_nn'
Cuts at: 0 677 2472
Size: 677 1795
BsrFI r'CCGG_y
Cuts at: 0 941 1389 2472
Size: 941 448 1083
Fragments arranged by size:
1083 941 448
BstAPI GCAn_nnn'nTGC
Cuts at: 0 909 1437 2205 2286 2472
Size: 909 528 768 81 186
Fragments arranged by size:
909 768 528 186 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 1299 2472
Size: 144 1155 1173
Fragments arranged by size:
1173 1155 144
BstYI r'GATC_y
Cuts at: 0 968 1541 1586 1763 2472
Size: 968 573 45 177 709
Fragments arranged by size:
968 709 573 177 45
Bsu36I CC'TnA_GG
Cuts at: 0 846 1682 2472
Size: 846 836 790
Fragments arranged by size:
846 836 790
BtgI C'CryG_G
Cuts at: 0 1472 2472
Size: 1472 1000
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2222 2472
Size: 2222 250
BtsI GCAGTG_nn'
Cuts at: 0 302 970 1724 2472
Size: 302 668 754 748
Fragments arranged by size:
754 748 668 302
DraIII CAC_nnn'GTG
Cuts at: 0 1047 2472
Size: 1047 1425
DrdI GACnn_nn'nnGTC
Cuts at: 0 1621 2472
Size: 1621 851
EaeI y'GGCC_r
Cuts at: 0 887 1869 2472
Size: 887 982 603
Fragments arranged by size:
982 887 603
EagI C'GGCC_G
Cuts at: 0 1869 2472
Size: 1869 603
EarI CTCTTCn'nnn_
Cuts at: 0 1568 2472
Size: 1568 904
EcoICRI GAG'CTC
Cuts at: 0 93 882 1578 2472
Size: 93 789 696 894
Fragments arranged by size:
894 789 696 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1647 1980 2440 2472
Size: 898 749 333 460 32
Fragments arranged by size:
898 749 460 333 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1609 2472
Size: 1609 863
EcoO109I rG'GnC_Cy
Cuts at: 0 1116 1744 2057 2366 2472
Size: 1116 628 313 309 106
Fragments arranged by size:
1116 628 313 309 106
EcoRI G'AATT_C
Cuts at: 0 250 748 2472
Size: 250 498 1724
Fragments arranged by size:
1724 498 250
EcoRV GAT'ATC
Cuts at: 0 1817 2472
Size: 1817 655
HaeII r_GCGC'y
Cuts at: 0 1910 2174 2472
Size: 1910 264 298
Fragments arranged by size:
1910 298 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 942 974 1093 1125 1132 1164
Size: 448 32 462 32 119 32 7 32
Cuts at: 1164 2090 2122 2472
Size: 926 32 350
Fragments arranged by size:
926 462 448 350 119 32 32 32
32 32 7
HincII GTy'rAC
Cuts at: 0 732 2472
Size: 732 1740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 248 344 571 1643 2472
Size: 125 123 96 227 1072 829
Fragments arranged by size:
1072 829 227 125 123 96
MscI TGG'CCA
Cuts at: 0 889 2472
Size: 889 1583
MslI CAynn'nnrTG
Cuts at: 0 869 1264 2472
Size: 869 395 1208
Fragments arranged by size:
1208 869 395
MspA1I CmG'CkG
Cuts at: 0 261 1533 1648 1714 1868 1915 2472
Size: 261 1272 115 66 154 47 557
Fragments arranged by size:
1272 557 261 154 115 66 47
NaeI GCC'GGC
Cuts at: 0 1391 2472
Size: 1391 1081
NcoI C'CATG_G
Cuts at: 0 1472 2472
Size: 1472 1000
NdeI CA'TA_TG
Cuts at: 0 443 2472
Size: 443 2029
NgoMIV G'CCGG_C
Cuts at: 0 1389 2472
Size: 1389 1083
NotI GC'GGCC_GC
Cuts at: 0 1869 2472
Size: 1869 603
NspI r_CATG'y
Cuts at: 0 1503 2472
Size: 1503 969
PasI CC'CwG_GG
Cuts at: 0 1824 2370 2472
Size: 1824 546 102
Fragments arranged by size:
1824 546 102
PfoI T'CCnGG_A
Cuts at: 0 1589 1610 2472
Size: 1589 21 862
Fragments arranged by size:
1589 862 21
PpuMI rG'GwC_Cy
Cuts at: 0 2057 2366 2472
Size: 2057 309 106
Fragments arranged by size:
2057 309 106
PsiI TTA'TAA
Cuts at: 0 425 2472
Size: 425 2047
PspOMI G'GGCC_C
Cuts at: 0 1743 2472
Size: 1743 729
PstI C_TGCA'G
Cuts at: 0 980 1457 1550 1640 1719 2249 2291 2472
Size: 980 477 93 90 79 530 42 181
Fragments arranged by size:
980 530 477 181 93 90 79 42
PvuII CAG'CTG
Cuts at: 0 261 1648 1714 1915 2472
Size: 261 1387 66 201 557
Fragments arranged by size:
1387 557 261 201 66
SacI G_AGCT'C
Cuts at: 0 95 884 1580 2472
Size: 95 789 696 892
Fragments arranged by size:
892 789 696 95
SanDI GG'GwC_CC
Cuts at: 0 2366 2472
Size: 2366 106
SapI GCTCTTCn'nnn_
Cuts at: 0 1568 2472
Size: 1568 904
SbfI CC_TGCA'GG
Cuts at: 0 1550 2291 2472
Size: 1550 741 181
Fragments arranged by size:
1550 741 181
SfcI C'TryA_G
Cuts at: 0 976 1453 1546 1636 1699 1715 2245 2287
Size: 976 477 93 90 63 16 530 42
Cuts at: 2287 2472
Size: 185
Fragments arranged by size:
976 530 477 185 93 90 63 42
16
SmaI CCC'GGG
Cuts at: 0 1365 2055 2472
Size: 1365 690 417
Fragments arranged by size:
1365 690 417
SmlI C'TyrA_G
Cuts at: 0 1149 1233 2472
Size: 1149 84 1239
Fragments arranged by size:
1239 1149 84
StuI AGG'CCT
Cuts at: 0 2453 2472
Size: 2453 19
StyI C'CwwG_G
Cuts at: 0 348 1472 2472
Size: 348 1124 1000
Fragments arranged by size:
1124 1000 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 271 1180 1687 2472
Size: 271 909 507 785
Fragments arranged by size:
909 785 507 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1269 1301 1418 1450 1452 1484 2472
Size: 1269 32 117 32 2 32 988
Fragments arranged by size:
1269 988 117 32 32 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 690 1296 2472
Size: 690 606 1176
Fragments arranged by size:
1176 690 606
XmaI C'CCGG_G
Cuts at: 0 1363 2053 2472
Size: 1363 690 419
Fragments arranged by size:
1363 690 419
Enzymes that do cut:
AarI AcuI AgeI AhdI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NotI NspI PasI PfoI PpuMI PsiI
PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI
SmaI SmlI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AleI
AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI
BclI BfrBI BglI BmrI BmtI BsaI BsaAI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI EciI FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NarI NheI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI